FastQCFastQC Report
Thu 26 May 2016
SRR1547458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547458_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1367195
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96200.7036304257988071No Hit
GAGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT26620.19470521761709192No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25660.1876835418502847No Hit
AAGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT20360.1489180402210365No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC17410.12734101572928513No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT16270.11900277575620156No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT15770.11534565296098948Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15570.11388280384290464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGG302.1658052E-645.0000041
GTACACG302.1658052E-645.0000041
CAACGAC302.1658052E-645.00000412
CGCTCAG302.1658052E-645.00000412
CGGATTC302.1658052E-645.00000433
CTACGTT600.045.00000412
TCGCTCA302.1658052E-645.00000411
ACTACGG302.1658052E-645.0000042
GCTAGAT302.1658052E-645.0000049
TACTCGA302.1658052E-645.00000416
TATAGTT302.1658052E-645.00000435
ACGTAAG302.1658052E-645.0000041
CGCGTGG302.1658052E-645.00000419
ACGCGGA302.1658052E-645.00000439
AACGTAC253.891196E-545.034
AGTCCGA253.891196E-545.019
CGAACAC207.0337567E-445.016
CTCCGTC253.891196E-545.021
GTTTCGA253.891196E-545.08
CATGCGC453.8562575E-1045.035