##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547458_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1367195
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18676999257604	31.0	31.0	33.0	30.0	34.0
2	31.581050983948888	31.0	31.0	34.0	30.0	34.0
3	31.698198867023358	31.0	31.0	34.0	30.0	34.0
4	35.460789426526574	37.0	35.0	37.0	33.0	37.0
5	35.32237903152074	37.0	35.0	37.0	33.0	37.0
6	35.36221241300619	37.0	35.0	37.0	33.0	37.0
7	35.837323132398815	37.0	35.0	37.0	35.0	37.0
8	35.869742794553815	37.0	35.0	37.0	35.0	37.0
9	37.62184253160668	39.0	37.0	39.0	35.0	39.0
10	36.89629496889617	39.0	37.0	39.0	32.0	39.0
11	36.61446099495683	39.0	35.0	39.0	32.0	39.0
12	35.907106155303374	37.0	35.0	39.0	31.0	39.0
13	35.56128130954253	37.0	35.0	39.0	30.0	39.0
14	36.631138206327556	38.0	35.0	41.0	31.0	41.0
15	36.89956516809965	38.0	35.0	41.0	31.0	41.0
16	37.02064811530177	38.0	35.0	41.0	32.0	41.0
17	36.9524866606446	38.0	35.0	41.0	32.0	41.0
18	36.871983147978156	38.0	35.0	40.0	32.0	41.0
19	36.74167474281284	38.0	35.0	40.0	31.0	41.0
20	36.57548703732825	38.0	35.0	40.0	31.0	41.0
21	36.477449815132445	38.0	35.0	40.0	31.0	41.0
22	36.36043212562948	38.0	35.0	40.0	31.0	41.0
23	36.30822669772783	38.0	35.0	40.0	31.0	41.0
24	36.18061432348714	37.0	35.0	40.0	31.0	41.0
25	36.09701834778507	37.0	35.0	40.0	30.0	41.0
26	35.960391165854176	37.0	35.0	40.0	30.0	41.0
27	35.870604412684365	37.0	34.0	40.0	30.0	41.0
28	35.85559192361002	37.0	35.0	40.0	30.0	41.0
29	35.84950866555246	37.0	35.0	40.0	30.0	41.0
30	35.76716635154459	37.0	35.0	40.0	30.0	41.0
31	35.60829289165042	37.0	34.0	40.0	30.0	41.0
32	35.39249631544878	37.0	34.0	40.0	29.0	41.0
33	35.21659602324467	37.0	34.0	40.0	27.0	41.0
34	35.06882924527957	37.0	34.0	40.0	27.0	41.0
35	34.934667695537215	37.0	34.0	40.0	26.0	41.0
36	34.81002636785535	37.0	34.0	40.0	25.0	41.0
37	34.72959380337114	37.0	34.0	40.0	24.0	41.0
38	34.62123471779812	37.0	33.0	40.0	24.0	41.0
39	34.5401343626915	37.0	34.0	40.0	24.0	41.0
40	34.3590746016479	37.0	33.0	40.0	23.0	41.0
41	34.283108115521195	36.0	33.0	40.0	23.0	41.0
42	34.21917868336265	36.0	33.0	40.0	23.0	41.0
43	34.17273761241081	36.0	33.0	40.0	23.0	41.0
44	34.039973815000785	36.0	33.0	40.0	23.0	41.0
45	33.90721660041179	36.0	33.0	40.0	22.0	41.0
46	33.84839836307184	36.0	33.0	40.0	22.0	41.0
47	33.801585728444	35.0	33.0	40.0	22.0	41.0
48	33.76279462695519	35.0	33.0	40.0	22.0	41.0
49	33.728355501592674	36.0	33.0	40.0	22.0	41.0
50	33.55846971353757	35.0	33.0	40.0	21.0	41.0
51	32.96852314410161	35.0	32.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	4.0
14	13.0
15	39.0
16	176.0
17	387.0
18	861.0
19	1646.0
20	2851.0
21	4565.0
22	6734.0
23	9930.0
24	14392.0
25	20214.0
26	24837.0
27	27674.0
28	30442.0
29	34496.0
30	40124.0
31	47120.0
32	57049.0
33	71842.0
34	116067.0
35	182658.0
36	99291.0
37	115450.0
38	164528.0
39	293539.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.29561254978258	25.37611679387359	28.21916405487147	14.109106601472357
2	29.036604142057275	27.722965634017093	30.401515511686334	12.838914712239294
3	27.9765505286371	26.895358745460594	32.146840794473356	12.981249931428948
4	23.956860579507676	30.08692980884219	31.766207453947683	14.19000215770245
5	22.513394212237465	33.02418455304474	30.700887583702396	13.761533651015402
6	20.439439875072686	42.15740987935152	27.16920410036608	10.233946145209718
7	86.74570928068052	3.98948211484097	7.723550773664327	1.541257830814185
8	86.2489988626348	3.8242533069532874	6.674395386173881	3.25235244423802
9	81.90323984508427	6.486053562220459	9.037993848719458	2.5727127439758046
10	40.345890673971155	32.78815384784175	14.752979640797397	12.112975837389692
11	35.959025596202444	24.853294519070065	24.890889741404848	14.296790143322644
12	35.17698645767429	24.03951155467947	25.429949641419107	15.35355234622713
13	20.58506650477803	40.594648166501486	24.71505527741105	14.105230051309434
14	15.200318901107742	40.33945413785159	26.8730502964098	17.587176664630867
15	13.63514348721287	27.95373008239498	42.52868098552145	15.882445444870703
16	14.959021939079648	25.00031085543759	41.214018483098606	18.82664872238415
17	14.507367273870955	26.8678571820406	27.9731128332096	30.651662710878842
18	18.17897227535209	27.463456200468844	33.790644348465285	20.56692717571378
19	23.266542080683443	30.058257966127726	26.474716481555298	20.200483471633525
20	25.857540438635308	27.375100113736515	26.95694469333197	19.810414754296204
21	19.62338949454906	32.62387589188082	29.101408357988433	18.651326255581687
22	21.577170776663166	27.805104611997557	25.136502108331293	25.481222503007984
23	17.440233470719246	32.77498820577899	25.608636661193174	24.176141662308595
24	16.10574936274635	28.19861102476238	38.67465869901513	17.020980913476134
25	15.68013341183957	29.871964130939627	33.99756435621839	20.450338101002416
26	15.571151152542248	37.22804720614104	27.404942235745448	19.79585940557126
27	15.154385438799878	38.53049491842788	27.285281177886112	19.029838464886137
28	14.383025098833743	33.373951777178824	33.663230190280096	18.579792933707335
29	14.279967378464667	28.29984018373385	32.71647424105559	24.703718196745893
30	16.948643024586836	35.79255336656439	28.35689129933916	18.901912309509616
31	23.569132420759292	32.94848211118385	24.68769999890286	18.794685469154
32	25.35666090060306	31.068794136900735	25.253164325498556	18.32138063699765
33	24.641035112035954	31.44043095535019	24.67416864456058	19.244365288053277
34	17.826791350173167	31.0733289691668	26.38438554851356	24.715494132146475
35	18.387135704855563	30.34658552730225	29.353384118578553	21.912894649263638
36	25.718862342240868	29.768979553026455	25.47858937459543	19.03356873013725
37	17.418290733947973	36.58234560541839	28.504785345177535	17.494578315456096
38	18.98822040747662	34.11130087514949	26.28191296779172	20.618565749582174
39	18.74385146230055	33.52660008265097	27.36281218114461	20.366736273903868
40	22.833099886994905	28.633442925113094	27.08355428450221	21.449902903389788
41	16.53509557890425	27.638778667271314	29.036896711880892	26.789229041943543
42	20.083162972363123	28.115082340119734	26.043175991720275	25.758578695796867
43	19.566265236487844	28.415112694238935	27.54018263671239	24.478439432560826
44	18.29380593112175	30.88527971503699	27.49776001228793	23.323154341553327
45	17.38925317895399	36.54255610940649	23.191059066190263	22.877131645449257
46	20.93849085170733	32.51372335328903	25.917882964756306	20.62990283024733
47	19.273622270414975	29.516784365068627	26.864931483804433	24.344661880711968
48	19.041248688007197	27.774384780517774	32.30921704658078	20.875149484894255
49	20.231788442760543	26.80122440471184	30.854486741101304	22.112500411426314
50	19.124996800017556	31.756772077136038	28.24608047864423	20.87215064420218
51	16.398172901451513	30.92024180895922	27.920377122502643	24.761208167086625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1687.0
1	1891.5
2	2096.0
3	7427.0
4	12758.0
5	8872.5
6	4987.0
7	5838.5
8	6690.0
9	7974.5
10	9259.0
11	10422.0
12	11585.0
13	12087.5
14	12590.0
15	12260.5
16	11931.0
17	11457.0
18	10983.0
19	10601.0
20	10219.0
21	9934.0
22	9649.0
23	10301.5
24	10954.0
25	10724.0
26	11383.5
27	12273.0
28	16315.0
29	20357.0
30	21317.0
31	22277.0
32	29546.5
33	36816.0
34	39733.0
35	42650.0
36	46776.5
37	50903.0
38	57814.5
39	64726.0
40	78714.5
41	92703.0
42	108449.5
43	124196.0
44	133908.0
45	143620.0
46	142176.0
47	140732.0
48	137694.5
49	134657.0
50	119226.5
51	103796.0
52	88820.0
53	73844.0
54	64058.0
55	54272.0
56	48827.0
57	43382.0
58	35236.5
59	27091.0
60	23777.0
61	20463.0
62	16535.0
63	12607.0
64	10480.5
65	8354.0
66	6991.0
67	5628.0
68	4066.5
69	2505.0
70	1873.0
71	1241.0
72	986.0
73	731.0
74	575.0
75	361.5
76	304.0
77	298.0
78	292.0
79	320.5
80	349.0
81	224.5
82	100.0
83	55.0
84	10.0
85	9.0
86	8.0
87	6.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1367195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.23772853437303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02463067416078	16.783036537869144
2	9.045013357953653	3.8419107657199505
3	3.331162794520301	2.1223899340147674
4	1.6721685516126492	1.4205224705146073
5	1.0156380791905635	1.0784922907510621
6	0.6447222688724197	0.8215461915826503
7	0.44969757473441496	0.6685388510342802
8	0.3242254126924481	0.550864903896582
9	0.2644201846863395	0.5054115691239531
>10	2.184656641368277	10.432821197122816
>50	0.8000892345989776	12.479062044646051
>100	1.2331074206798505	46.318566111079456
>500	0.005931756126643305	0.8052896149075192
>1k	0.004187121971748215	1.458665756863864
>5k	3.489268309790179E-4	0.7128817608733169
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9620	0.7036304257988071	No Hit
GAGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	2662	0.19470521761709192	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2566	0.1876835418502847	No Hit
AAGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	2036	0.1489180402210365	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	1741	0.12734101572928513	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	1627	0.11900277575620156	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	1577	0.11534565296098948	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1557	0.11388280384290464	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12873072239146574	0.0
2	0.0	0.0	0.0	0.4685505725225736	0.0
3	0.0	0.0	0.0	0.6958773254729574	0.0
4	0.0	0.0	0.0	1.3641799450700156	0.0
5	0.0	0.0	0.0	2.0888753981692445	0.0
6	0.0	0.0	0.0	3.100801275604431	0.0
7	0.0	0.0	0.0	3.464319281448513	0.0
8	0.0	0.0	0.0	4.443331053726791	0.0
9	0.0	0.0	0.0	4.702401632539615	0.0
10	0.0	0.0	0.0	5.275911629284776	0.0
11	0.0	0.0	0.0	6.0993493978547315	0.0
12	0.0	0.0	0.0	6.699410106093132	0.0
13	0.0	0.0	0.0	6.94421790600463	0.0
14	0.0	0.0	0.0	7.040692805342325	0.0
15	0.0	0.0	0.0	7.195901096771126	0.0
16	0.0	0.0	0.0	7.572145889942546	0.0
17	0.0	0.0	0.0	8.012024619750658	0.0
18	0.0	0.0	0.0	8.510783026561683	0.0
19	0.0	0.0	0.0	8.762466217328178	0.0
20	0.0	0.0	0.0	8.990378109925796	0.0
21	0.0	0.0	0.0	9.32390770884914	0.0
22	0.0	0.0	0.0	9.6577298775961	0.0
23	0.0	0.0	0.0	10.030390690428213	0.0
24	0.0	0.0	0.0	10.295458950625186	0.0
25	0.0	0.0	0.0	10.529149097239237	0.0
26	0.0	0.0	0.0	10.745138769524464	0.0
27	0.0	0.0	0.0	10.94437881940762	0.0
28	0.0	0.0	0.0	11.161611913443219	0.0
29	0.0	0.0	0.0	11.409491696502693	0.0
30	0.0	0.0	0.0	11.709595193077798	0.0
31	0.0	0.0	0.0	11.997264472149181	0.0
32	0.0	0.0	0.0	12.233587747175786	0.0
33	0.0	0.0	0.0	12.458061944345905	0.0
34	0.0	0.0	0.0	12.698261769535435	0.0
35	0.0	0.0	0.0	12.97854366056049	0.0
36	0.0	0.0	0.0	13.238491948844166	0.0
37	0.0	0.0	0.0	13.503340781673426	0.0
38	7.31424559042419E-5	0.0	0.0	13.75378055068955	0.0
39	7.31424559042419E-5	0.0	0.0	13.996320934468017	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGG	30	2.1658052E-6	45.000004	1
GTACACG	30	2.1658052E-6	45.000004	1
CAACGAC	30	2.1658052E-6	45.000004	12
CGCTCAG	30	2.1658052E-6	45.000004	12
CGGATTC	30	2.1658052E-6	45.000004	33
CTACGTT	60	0.0	45.000004	12
TCGCTCA	30	2.1658052E-6	45.000004	11
ACTACGG	30	2.1658052E-6	45.000004	2
GCTAGAT	30	2.1658052E-6	45.000004	9
TACTCGA	30	2.1658052E-6	45.000004	16
TATAGTT	30	2.1658052E-6	45.000004	35
ACGTAAG	30	2.1658052E-6	45.000004	1
CGCGTGG	30	2.1658052E-6	45.000004	19
ACGCGGA	30	2.1658052E-6	45.000004	39
AACGTAC	25	3.891196E-5	45.0	34
AGTCCGA	25	3.891196E-5	45.0	19
CGAACAC	20	7.0337567E-4	45.0	16
CTCCGTC	25	3.891196E-5	45.0	21
GTTTCGA	25	3.891196E-5	45.0	8
CATGCGC	45	3.8562575E-10	45.0	35
>>END_MODULE