FastQCFastQC Report
Thu 26 May 2016
SRR1547457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547457_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences488410
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54121.1080854200364447No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6690.13697508241027007No Hit
GGTAATGGGGAAAAGAAAGAAGGAGGGAAAAGAAGGAGGAAAAAGAAAGAA5460.11179132286398723No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5360.10974386273827318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5250.10749165659998772No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC253.8874994E-545.06
TTAGGCG253.8874994E-545.01
AACCGCA207.0292986E-445.015
CGGCATA207.0292986E-445.033
GACGTCG406.8012014E-945.019
CGTTGCA207.0292986E-445.018
GACAACG207.0292986E-445.01
TAGCGAG502.1827873E-1145.01
TCTTACG207.0292986E-445.032
TGAGTCG207.0292986E-445.01
CGCGACC253.8874994E-545.010
TAACGAG207.0292986E-445.01
TGCGGAT207.0292986E-445.04
CGTAGGT207.0292986E-445.03
TACGAAT453.8380676E-1045.012
TGCGACC207.0292986E-445.043
TCGTCCC700.045.038
CGAATAT453.8380676E-1045.014
TCCGTTG207.0292986E-445.016
AGCGACG406.8012014E-945.016