##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547457_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	488410
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02667226305768	33.0	31.0	33.0	31.0	34.0
2	32.526473659425484	33.0	31.0	34.0	31.0	34.0
3	32.642361950001025	34.0	31.0	34.0	31.0	34.0
4	36.24865993734772	37.0	35.0	37.0	35.0	37.0
5	36.21991154972257	37.0	37.0	37.0	35.0	37.0
6	36.26446837697836	37.0	37.0	37.0	35.0	37.0
7	36.3610880203108	37.0	37.0	37.0	35.0	37.0
8	36.37137855490264	37.0	37.0	37.0	35.0	37.0
9	38.20463954464487	39.0	39.0	39.0	37.0	39.0
10	37.9134006265228	39.0	38.0	39.0	35.0	39.0
11	37.80533568108761	39.0	38.0	39.0	35.0	39.0
12	37.52348232018181	39.0	37.0	39.0	35.0	39.0
13	37.429184496631926	39.0	37.0	39.0	35.0	39.0
14	38.815796154869886	40.0	38.0	41.0	35.0	41.0
15	38.86839950041973	40.0	38.0	41.0	35.0	41.0
16	38.87450502651461	40.0	38.0	41.0	35.0	41.0
17	38.84041891034172	40.0	38.0	41.0	35.0	41.0
18	38.69112835527528	40.0	38.0	41.0	35.0	41.0
19	38.58950062447534	40.0	37.0	41.0	35.0	41.0
20	38.44572387952745	40.0	36.0	41.0	35.0	41.0
21	38.41259802215352	40.0	36.0	41.0	35.0	41.0
22	38.32929505947872	40.0	36.0	41.0	35.0	41.0
23	38.22736839950042	40.0	36.0	41.0	34.0	41.0
24	38.154939497553286	40.0	36.0	41.0	34.0	41.0
25	38.02741139616306	40.0	35.0	41.0	34.0	41.0
26	37.95174341229705	40.0	35.0	41.0	34.0	41.0
27	37.88258839909093	40.0	35.0	41.0	34.0	41.0
28	37.79179787473639	40.0	35.0	41.0	34.0	41.0
29	37.808535861264104	40.0	35.0	41.0	34.0	41.0
30	37.71990335988207	40.0	35.0	41.0	34.0	41.0
31	37.59175487807375	40.0	35.0	41.0	34.0	41.0
32	37.4546856124977	40.0	35.0	41.0	33.0	41.0
33	37.30953706926557	40.0	35.0	41.0	33.0	41.0
34	37.14878892733564	40.0	35.0	41.0	33.0	41.0
35	36.97456645031838	40.0	35.0	41.0	33.0	41.0
36	36.893153293339616	40.0	35.0	41.0	32.0	41.0
37	36.827763559304685	40.0	35.0	41.0	32.0	41.0
38	36.6943387727524	40.0	35.0	41.0	31.0	41.0
39	36.5607215249483	40.0	35.0	41.0	31.0	41.0
40	36.42292745848775	40.0	35.0	41.0	30.0	41.0
41	36.32051759791978	40.0	35.0	41.0	30.0	41.0
42	36.23896930857271	40.0	35.0	41.0	30.0	41.0
43	36.17000470915829	40.0	35.0	41.0	30.0	41.0
44	36.02516533240515	39.0	35.0	41.0	29.0	41.0
45	35.81334944001966	39.0	35.0	41.0	27.0	41.0
46	35.804131774533694	39.0	35.0	41.0	28.0	41.0
47	35.80670747937184	39.0	35.0	41.0	28.0	41.0
48	35.72897360823898	39.0	35.0	41.0	28.0	41.0
49	35.63897545095309	39.0	35.0	41.0	27.0	41.0
50	35.53564628078868	39.0	35.0	41.0	27.0	41.0
51	35.04099424663705	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	4.0
15	12.0
16	23.0
17	24.0
18	75.0
19	158.0
20	311.0
21	520.0
22	933.0
23	1526.0
24	2711.0
25	4286.0
26	5631.0
27	6148.0
28	6175.0
29	6449.0
30	7018.0
31	7603.0
32	9260.0
33	12352.0
34	23693.0
35	45715.0
36	33581.0
37	42126.0
38	76203.0
39	195710.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.503654716324398	25.45525275895252	29.76106140332917	13.280031121393913
2	31.738088900718658	27.89050183247681	27.558403800085994	12.813005466718536
3	28.970741794803544	25.784484347167343	32.08861407424091	13.15615978378821
4	25.52834708544051	28.943510575131548	30.768002293155337	14.7601400462726
5	23.477815769537887	33.29682029442477	29.71888372473946	13.506480211297886
6	21.26573984971643	42.146557195798614	26.839950041972934	9.747752912512029
7	85.82604778771935	4.663909420364038	7.787514588153396	1.7225282037632315
8	84.37071313036178	5.340390245899961	7.541000389017424	2.747896234720829
9	78.39888618169161	9.251858070064085	9.474212239716632	2.8750435085276713
10	32.58778485288999	37.080117114719194	17.807989189410538	12.524108842980283
11	31.795213038226077	27.673675805163693	24.753997665895458	15.777113490714767
12	27.706844659200264	25.840584754611907	28.573944022440163	17.87862656374767
13	22.171740955344895	31.97579902131406	28.465019143752173	17.38744087958887
14	18.372678692082474	33.81585143629328	29.350136156098362	18.46133371552589
15	17.71667246780369	31.290309371224996	34.80620789910117	16.18681026187015
16	19.52621772690977	28.91464138735898	34.286767265207516	17.27237362052374
17	19.807129256157737	30.227882311991973	28.064535943162504	21.900452488687783
18	19.74038205605946	30.561823058495936	31.1334739255953	18.564320959849308
19	22.958784627669377	31.07245961384902	27.98161380807109	17.987141950410514
20	23.257304313998482	30.096025879895986	29.403165373354355	17.243504432751173
21	21.71761429946152	31.77371470690608	29.720931184865172	16.787739808767224
22	22.217604062160888	28.558178579472166	28.020925042484794	21.20329231588215
23	19.10935484531439	32.70919923834483	28.659118363669865	19.522327552670912
24	18.32149218893962	30.632050940807925	33.148379435310495	17.898077434941957
25	18.223623594930487	32.08963780430376	30.94858827624332	18.73815032452243
26	17.80307528510882	35.16512765913884	28.241027005999058	18.79077004975328
27	17.949878176122517	35.358203148993674	28.85014639339899	17.84177228148482
28	16.963616633566062	33.91208206220184	31.801560164615793	17.32274113961631
29	17.437603652668866	30.58823529411765	31.374664728404415	20.599496324809074
30	18.45723879527446	34.76976310886346	28.82004872955099	17.952949366311092
31	21.579820233000962	32.511824082226	27.29305296779345	18.61530271697959
32	21.1019430396593	32.363792715136874	28.52234802727217	18.011916217931656
33	21.38572101308327	31.874654491103787	26.691713928871234	20.04791056694171
34	18.893143056038983	32.276570913781455	28.216457484490487	20.613828545689074
35	19.732601707581747	30.900882455314182	28.647652586965872	20.718863250138202
36	22.28598923035974	32.39143342683401	26.92962879547921	18.39294854732704
37	18.377183104359045	35.211195511967404	27.824983108453967	18.58663827521959
38	19.548944534305193	33.592268790565306	26.93843287401978	19.920353801109727
39	19.58170389631662	32.12239716631519	29.017219139657257	19.27867979771094
40	21.42380377142155	31.767777072541513	27.17020536025061	19.638213795786328
41	18.466657111852747	30.152535779365696	29.18982002825495	22.190987080526607
42	19.383305010134926	30.474396511127942	27.652586965868842	22.489711512868286
43	20.04647734485371	29.960279273561145	28.837656886632136	21.15558649495301
44	18.48856493519789	32.332876067238594	28.514362932781882	20.66419606478164
45	19.25718146639094	33.87338506582584	26.307405663274707	20.562027804508507
46	20.325750906001105	33.42151061608075	26.728158719108947	19.524579758809196
47	20.56120882045822	30.57840748551422	28.24737413238877	20.613009561638787
48	19.53809299563891	31.12364611699187	29.895579533588585	19.442681353780635
49	19.88309002682173	29.84357404639545	30.051800741180568	20.22153518560226
50	18.514772424807028	32.186277922237466	29.71724575663889	19.58170389631662
51	18.668536680248152	30.915214676194182	28.334186441719044	22.08206220183862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	720.0
1	941.0
2	1162.0
3	4357.5
4	7553.0
5	5065.0
6	2577.0
7	2803.5
8	3030.0
9	3602.5
10	4175.0
11	4744.5
12	5314.0
13	5527.5
14	5741.0
15	5736.5
16	5732.0
17	5344.0
18	4956.0
19	4801.0
20	4646.0
21	4570.5
22	4495.0
23	4557.5
24	4620.0
25	4943.5
26	6682.5
27	8098.0
28	8861.5
29	9625.0
30	10501.0
31	11377.0
32	12818.5
33	14260.0
34	16529.5
35	18799.0
36	19715.0
37	20631.0
38	22747.5
39	24864.0
40	27437.0
41	30010.0
42	32358.0
43	34706.0
44	35655.0
45	36604.0
46	37365.5
47	38127.0
48	39243.5
49	40360.0
50	37696.5
51	35033.0
52	30702.5
53	26372.0
54	23743.5
55	21115.0
56	18680.5
57	16246.0
58	14928.0
59	13610.0
60	11794.5
61	9979.0
62	8747.5
63	7516.0
64	6118.0
65	4720.0
66	3655.0
67	2590.0
68	2013.0
69	1436.0
70	1182.0
71	928.0
72	693.0
73	458.0
74	470.5
75	388.0
76	293.0
77	213.5
78	134.0
79	82.5
80	31.0
81	19.0
82	7.0
83	6.0
84	5.0
85	3.0
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	488410.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.297816635530985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38885521865721	19.071734356841834
2	7.432482377122765	3.7605115264653435
3	2.9754354478530516	2.2581606111193655
4	1.8127740436950768	1.8343690143619233
5	1.1619936879034092	1.4697951624112433
6	0.8815231974061548	1.3380367324768734
7	0.6651082034185933	1.1778049761009712
8	0.5707688997616197	1.1551365573946577
9	0.4935237538953013	1.1236566088190028
>10	7.443488540141809	44.928450603074346
>50	1.1114416505597202	17.732662616278258
>100	0.05853972117066559	2.568380152523014
>500	0.0032522067317036435	0.46813752119343155
>1k	0.0	0.0
>5k	8.130516829259109E-4	1.1131635609397414
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5412	1.1080854200364447	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	669	0.13697508241027007	No Hit
GGTAATGGGGAAAAGAAAGAAGGAGGGAAAAGAAGGAGGAAAAAGAAAGAA	546	0.11179132286398723	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	536	0.10974386273827318	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	525	0.10749165659998772	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06265227984684998	0.0
2	0.0	0.0	0.0	0.21703077332568949	0.0
3	0.0	0.0	0.0	0.3396736348559612	0.0
4	0.0	0.0	0.0	0.6373743371347843	0.0
5	0.0	0.0	0.0	1.0079646198890277	0.0
6	0.0	0.0	0.0	1.4592248315964047	0.0
7	0.0	0.0	0.0	1.6799410331483795	0.0
8	0.0	0.0	0.0	2.2272271247517454	0.0
9	0.0	0.0	0.0	2.456952150856862	0.0
10	0.0	0.0	0.0	2.879957412829385	0.0
11	0.0	0.0	0.0	3.4456706455641775	0.0
12	0.0	0.0	0.0	3.8694948915869865	0.0
13	0.0	0.0	0.0	4.041686288159538	0.0
14	0.0	0.0	0.0	4.118056550848673	0.0
15	0.0	0.0	0.0	4.222886509285232	0.0
16	0.0	0.0	0.0	4.440736266661207	0.0
17	0.0	0.0	0.0	4.702401670727463	0.0
18	0.0	0.0	0.0	5.027743084703426	0.0
19	0.0	0.0	0.0	5.211809750005119	0.0
20	0.0	0.0	0.0	5.395057431256526	0.0
21	0.0	0.0	0.0	5.619459061034786	0.0
22	0.0	0.0	0.0	5.840584754611904	0.0
23	0.0	0.0	0.0	6.08914641387359	0.0
24	0.0	0.0	0.0	6.273213079175283	0.0
25	0.0	0.0	0.0	6.440900063471264	0.0
26	0.0	0.0	0.0	6.592821604799247	0.0
27	0.0	0.0	0.0	6.747609590303229	0.0
28	0.0	0.0	0.0	6.895436211379783	0.0
29	0.0	0.0	0.0	7.064351671751193	0.0
30	0.0	0.0	0.0	7.261726827870027	0.0
31	0.0	0.0	0.0	7.454802317724862	0.0
32	0.0	0.0	0.0	7.639278475051698	0.0
33	0.0	0.0	0.0	7.835220409082533	0.0
34	0.0	0.0	0.0	8.027886406912225	0.0
35	0.0	0.0	0.0	8.228946991257345	0.0
36	0.0	0.0	0.0	8.42632214737618	0.0
37	0.0	0.0	0.0	8.629225445834443	0.0
38	0.0	0.0	0.0	8.852193853524703	0.0
39	0.0	0.0	0.0	9.062263262422965	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	25	3.8874994E-5	45.0	6
TTAGGCG	25	3.8874994E-5	45.0	1
AACCGCA	20	7.0292986E-4	45.0	15
CGGCATA	20	7.0292986E-4	45.0	33
GACGTCG	40	6.8012014E-9	45.0	19
CGTTGCA	20	7.0292986E-4	45.0	18
GACAACG	20	7.0292986E-4	45.0	1
TAGCGAG	50	2.1827873E-11	45.0	1
TCTTACG	20	7.0292986E-4	45.0	32
TGAGTCG	20	7.0292986E-4	45.0	1
CGCGACC	25	3.8874994E-5	45.0	10
TAACGAG	20	7.0292986E-4	45.0	1
TGCGGAT	20	7.0292986E-4	45.0	4
CGTAGGT	20	7.0292986E-4	45.0	3
TACGAAT	45	3.8380676E-10	45.0	12
TGCGACC	20	7.0292986E-4	45.0	43
TCGTCCC	70	0.0	45.0	38
CGAATAT	45	3.8380676E-10	45.0	14
TCCGTTG	20	7.0292986E-4	45.0	16
AGCGACG	40	6.8012014E-9	45.0	16
>>END_MODULE