FastQCFastQC Report
Thu 26 May 2016
SRR1547456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547456_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3953499
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333290.8430253808082411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT209300.041.8609661
CGTTAGG6500.039.8076932
TACGGGA27050.039.2606284
TTAGGGA76100.038.5249674
AGGGCGA35600.038.4269686
TAGGGCG15200.038.190795
TAGGGAT90600.038.1208575
GGGCGAT69750.037.9032257
ATAGGGA81400.037.868554
TAAGGGA79050.037.4003834
AGGGATT83950.037.3883256
AAGGGAC59450.037.279235
TCACGAC5100.037.05882325
TAGGGAC54900.037.0081985
GACCGAT25850.036.9922649
CGGTCTA5050.036.98019431
TGGGCGA17750.036.8873256
GGACCGA27300.036.7582448
TATGGGA70600.036.7138824
AAGGGAT89650.036.441725
Produced by FastQC (version 0.11.5)