##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547456_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3953499
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34257147908726	31.0	31.0	33.0	30.0	34.0
2	31.766493174780113	31.0	31.0	34.0	30.0	34.0
3	31.87617601522095	31.0	31.0	34.0	30.0	34.0
4	35.65470688117033	37.0	35.0	37.0	33.0	37.0
5	35.55125725338491	37.0	35.0	37.0	33.0	37.0
6	35.59757318770031	37.0	35.0	37.0	33.0	37.0
7	35.95147437750711	37.0	35.0	37.0	35.0	37.0
8	35.977395213708164	37.0	35.0	37.0	35.0	37.0
9	37.72263860443622	39.0	37.0	39.0	35.0	39.0
10	37.111674999791326	39.0	37.0	39.0	33.0	39.0
11	36.945354482194126	39.0	37.0	39.0	33.0	39.0
12	36.65843016527891	39.0	35.0	39.0	32.0	39.0
13	36.60581778318396	39.0	35.0	39.0	32.0	39.0
14	37.7628298375692	40.0	36.0	41.0	32.0	41.0
15	37.82847826697313	40.0	36.0	41.0	33.0	41.0
16	37.83409911068651	40.0	36.0	41.0	33.0	41.0
17	37.75332079254352	40.0	36.0	41.0	32.0	41.0
18	37.61018050086771	39.0	36.0	41.0	32.0	41.0
19	37.527051100809686	39.0	36.0	41.0	32.0	41.0
20	37.39898833919017	39.0	35.0	41.0	32.0	41.0
21	37.33633826643184	39.0	35.0	41.0	32.0	41.0
22	37.25315600181004	39.0	35.0	41.0	32.0	41.0
23	37.15347569330358	39.0	35.0	41.0	32.0	41.0
24	37.07932138088311	39.0	35.0	41.0	31.0	41.0
25	36.97949057278122	39.0	35.0	41.0	31.0	41.0
26	36.83727604332264	39.0	35.0	41.0	31.0	41.0
27	36.75391343212683	39.0	35.0	41.0	31.0	41.0
28	36.63500914000484	38.0	35.0	40.0	30.0	41.0
29	36.65425563532456	39.0	35.0	40.0	30.0	41.0
30	36.59545379928008	39.0	35.0	40.0	30.0	41.0
31	36.48685430298578	39.0	35.0	41.0	30.0	41.0
32	36.37310923817105	39.0	35.0	41.0	30.0	41.0
33	36.2276117940083	39.0	35.0	41.0	30.0	41.0
34	36.041857605123965	39.0	35.0	41.0	29.0	41.0
35	35.88131096024053	39.0	35.0	41.0	27.0	41.0
36	35.81880961649415	39.0	35.0	41.0	27.0	41.0
37	35.77662647695118	39.0	35.0	41.0	27.0	41.0
38	35.654417011361325	39.0	35.0	41.0	27.0	41.0
39	35.55811421730472	38.0	35.0	41.0	26.0	41.0
40	35.455778033584934	38.0	35.0	41.0	25.0	41.0
41	35.34276219622163	38.0	34.0	41.0	25.0	41.0
42	35.29937202462932	38.0	34.0	41.0	25.0	41.0
43	35.268337996291386	38.0	34.0	40.0	25.0	41.0
44	35.1432207267537	38.0	34.0	40.0	24.0	41.0
45	35.02145744819968	38.0	34.0	40.0	24.0	41.0
46	34.98478866442106	38.0	34.0	40.0	24.0	41.0
47	34.94482280127047	38.0	34.0	40.0	24.0	41.0
48	34.86258071647419	38.0	34.0	40.0	24.0	41.0
49	34.78323480036292	38.0	34.0	40.0	24.0	41.0
50	34.693172554236135	38.0	34.0	40.0	24.0	41.0
51	34.151039876322216	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	1.0
11	1.0
12	4.0
13	11.0
14	40.0
15	94.0
16	272.0
17	642.0
18	1464.0
19	2933.0
20	5208.0
21	8501.0
22	13050.0
23	19717.0
24	29765.0
25	44669.0
26	61000.0
27	70825.0
28	75710.0
29	82417.0
30	94592.0
31	112713.0
32	136885.0
33	170962.0
34	245289.0
35	317172.0
36	318669.0
37	407677.0
38	616582.0
39	1116004.0
40	627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.906546833577046	24.578050987239404	27.238656187847777	15.276745991335778
2	32.44723218596995	25.661673368325122	28.80504080056679	13.086053645138144
3	28.146409041712168	25.890331577167462	32.85605991047424	13.107199470646128
4	24.91957124562318	28.778279696036346	32.00387808369244	14.298270974648029
5	23.06521387763093	32.228514538640326	30.926983919813818	13.779287663914927
6	22.21318381514704	40.78478330208253	27.290964282525426	9.711068600245
7	88.99875780922166	2.5213614572812593	7.415987711138918	1.0638930223581693
8	89.08055876579203	2.2959914748935057	7.322652667927828	1.3007970913866427
9	82.61671496565447	6.445707966537996	8.803366334479913	2.134210733327617
10	27.28701841077992	48.17084815248467	14.037312264401736	10.504821172333672
11	29.666252603073882	24.20094200099709	29.67907668624679	16.453728709682235
12	28.57152613419151	22.792139317601954	29.74385474740224	18.892479800804303
13	24.841184985755657	25.11261543255734	31.263268309919894	18.78293127176711
14	21.149214910639916	27.31911655978666	31.360549224876493	20.171119304696926
15	19.933633472526488	26.560927421506875	34.26228260080501	19.24315650516163
16	23.9742061399282	26.143322661773787	32.30679962230925	17.575671575988764
17	23.338642554355015	25.83830171703597	30.342008433542034	20.48104729506698
18	23.128600766055587	24.96209054308601	32.38564117507049	19.523667515787913
19	24.3670733191029	26.77577507923993	29.572487561018733	19.284664040638432
20	25.57580006976099	26.567478580366405	30.008658153195434	17.84806319667717
21	25.26842677840566	25.438605144455583	31.381366227739022	17.911601849399734
22	24.412627902523816	24.064025310237845	31.13429395075097	20.389052836487377
23	21.40835244931136	26.78978798274642	31.547649310142738	20.254210257799485
24	20.75475420633722	26.29053403074087	33.061928180581305	19.892783582340606
25	21.35149142569658	28.00911294020815	31.021128372613727	19.618267261481538
26	21.516307453220552	28.70424401270874	29.882263787090878	19.897184746979825
27	20.26774257436261	27.965809527206154	31.410429090787677	20.356018807643558
28	19.763682752923422	27.712894324748788	32.486387374829235	20.037035547498558
29	20.452085608216926	26.111072748469137	32.43324457651311	21.00359706680083
30	21.297260983245476	27.12020415333354	31.662231355060417	19.92030350836057
31	22.436757920009594	27.172664012309095	30.304573240058996	20.086004827622318
32	22.5433723392873	27.480644360856044	30.14911601090578	19.826867288950876
33	22.750252371380387	27.253023208049378	29.55898053850526	20.437743882064975
34	21.049379296668597	27.907102037966876	30.598211862453994	20.445306802910533
35	21.382400754369737	27.882541515756042	30.423859978211702	20.311197751662515
36	22.853477388004904	28.205445353596904	28.634230083275597	20.306847175122595
37	21.30330626111199	29.79725048621487	28.483856958102177	20.41558629457096
38	21.279276913943825	29.027046674351	29.555667018001014	20.13800939370416
39	21.31332270477367	28.689598757960987	28.80071551807652	21.196363019188823
40	22.887371414536844	27.722151946920942	29.38171983855314	20.008756799989072
41	20.67603912382424	27.441135055301647	29.76305293108712	22.119772889786997
42	21.4784169668438	27.647939205245788	29.039061347935082	21.834582479975335
43	21.298829214323817	27.49926078139896	29.60982157830317	21.592088425974055
44	21.139805524169855	28.268326361028546	29.358373430725543	21.233494684076053
45	20.621833975422785	28.477508151639853	28.82765368095452	22.073004191982847
46	21.226209997776653	28.80253668965137	28.9008799546933	21.07037335787868
47	20.6548680042666	28.266075190609634	30.11304669610388	20.966010109019884
48	21.115067943611468	27.804433490434675	30.70409275429183	20.376405811662025
49	21.321062683966787	27.469641449257985	30.03043632994469	21.178859536830537
50	20.55649944517502	28.478899324370637	30.33168846128455	20.632912769169792
51	20.18348809497612	28.10338386325632	29.835646853584635	21.877481188182923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1934.0
1	3366.0
2	4798.0
3	30078.5
4	55359.0
5	39090.5
6	22822.0
7	22462.0
8	22102.0
9	22736.5
10	23371.0
11	23534.0
12	23697.0
13	23574.5
14	23452.0
15	22666.0
16	21880.0
17	21107.0
18	20334.0
19	20576.0
20	20818.0
21	21610.5
22	22403.0
23	23964.0
24	25525.0
25	30802.0
26	43570.0
27	51061.0
28	60420.0
29	69779.0
30	80238.0
31	90697.0
32	105893.0
33	121089.0
34	137414.5
35	153740.0
36	165408.5
37	177077.0
38	185182.0
39	193287.0
40	210017.5
41	226748.0
42	243072.0
43	259396.0
44	264815.0
45	270234.0
46	281403.0
47	292572.0
48	292296.5
49	292021.0
50	283048.0
51	274075.0
52	256760.0
53	239445.0
54	223799.0
55	208153.0
56	193419.5
57	178686.0
58	162104.5
59	145523.0
60	128519.5
61	111516.0
62	99772.0
63	88028.0
64	76710.0
65	65392.0
66	53984.5
67	42577.0
68	35526.5
69	28476.0
70	23640.5
71	18805.0
72	15625.5
73	12446.0
74	10147.5
75	6388.5
76	4928.0
77	3736.0
78	2544.0
79	1976.5
80	1409.0
81	1145.5
82	882.0
83	592.5
84	303.0
85	205.5
86	108.0
87	70.5
88	33.0
89	28.5
90	24.0
91	18.5
92	13.0
93	10.5
94	8.0
95	4.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3953499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.92387583995797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6875571373711	13.9246212843988
2	6.783323579754243	2.4316689925154855
3	2.372352794730131	1.2756527082396047
4	1.3052381961280712	0.9357970947588102
5	0.8534360355318907	0.7648440769109782
6	0.6323075804828501	0.68000415391433
7	0.4845744227097025	0.6079816251507612
8	0.4328233953809561	0.6206298239549836
9	0.355655221982734	0.5737248036586092
>10	5.706140471180107	26.461546625187005
>50	2.652175934504041	33.967476247188806
>100	0.7308351586499686	16.354863548005643
>500	0.0025776515478136645	0.3091137446617904
>1k	8.592171826045482E-4	0.23660304727164064
>5k	0.0	0.0
>10k+	1.4320286376742473E-4	0.8554722241827413
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33329	0.8430253808082411	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.0588099301403645E-5	0.0	0.0	0.029518155942369024	0.0
2	5.0588099301403645E-5	0.0	0.0	0.07565450250524915	0.0
3	5.0588099301403645E-5	0.0	0.0	0.12201849551498559	0.0
4	5.0588099301403645E-5	0.0	0.0	0.1989124064531191	0.0
5	5.0588099301403645E-5	0.0	0.0	0.32558500710383387	0.0
6	5.0588099301403645E-5	0.0	0.0	0.5840649004843558	0.0
7	5.0588099301403645E-5	0.0	0.0	0.7019857599559276	0.0
8	5.0588099301403645E-5	0.0	0.0	0.9945873263152463	0.0
9	5.0588099301403645E-5	0.0	0.0	1.1338563636920105	0.0
10	5.0588099301403645E-5	0.0	0.0	1.3722780756995259	0.0
11	5.0588099301403645E-5	0.0	0.0	1.6082715589405738	0.0
12	5.0588099301403645E-5	0.0	0.0	1.8079680809328649	0.0
13	5.0588099301403645E-5	0.0	0.0	1.9173648456721502	0.0
14	5.0588099301403645E-5	0.0	0.0	1.9780705648338346	0.0
15	5.0588099301403645E-5	0.0	0.0	2.045125090457845	0.0
16	5.0588099301403645E-5	0.0	0.0	2.1555083231335077	0.0
17	5.0588099301403645E-5	0.0	0.0	2.2753009422792316	0.0
18	5.0588099301403645E-5	0.0	0.0	2.407614116002053	0.0
19	5.0588099301403645E-5	0.0	0.0	2.4944738825025627	0.0
20	5.0588099301403645E-5	0.0	0.0	2.5951189060627056	0.0
21	5.0588099301403645E-5	0.0	0.0	2.714000939421004	0.0
22	5.0588099301403645E-5	0.0	0.0	2.8502093967900333	0.0
23	5.0588099301403645E-5	0.0	0.0	2.99640900377109	0.0
24	5.0588099301403645E-5	0.0	0.0	3.112356927369907	0.0
25	5.0588099301403645E-5	0.0	0.0	3.2148231225049	0.0
26	5.0588099301403645E-5	0.0	0.0	3.316606378299324	0.0
27	5.0588099301403645E-5	0.0	0.0	3.427419609819049	0.0
28	5.0588099301403645E-5	0.0	0.0	3.5408634225024467	0.0
29	5.0588099301403645E-5	0.0	0.0	3.666625437365736	0.0
30	5.0588099301403645E-5	0.0	0.0	3.8161132708013836	0.0
31	5.0588099301403645E-5	0.0	0.0	3.947338800389225	0.0
32	5.0588099301403645E-5	0.0	0.0	4.0789184466721755	0.0
33	7.588214895210547E-5	0.0	0.0	4.21062456320338	0.0
34	7.588214895210547E-5	0.0	0.0	4.349868306530493	0.0
35	7.588214895210547E-5	0.0	0.0	4.500469078150772	0.0
36	7.588214895210547E-5	0.0	0.0	4.646112216039513	0.0
37	7.588214895210547E-5	0.0	0.0	4.7953724030283045	0.0
38	7.588214895210547E-5	0.0	0.0	4.960871369892847	0.0
39	7.588214895210547E-5	0.0	0.0	5.1911736919624865	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	20930	0.0	41.860966	1
CGTTAGG	650	0.0	39.807693	2
TACGGGA	2705	0.0	39.260628	4
TTAGGGA	7610	0.0	38.524967	4
AGGGCGA	3560	0.0	38.426968	6
TAGGGCG	1520	0.0	38.19079	5
TAGGGAT	9060	0.0	38.120857	5
GGGCGAT	6975	0.0	37.903225	7
ATAGGGA	8140	0.0	37.86855	4
TAAGGGA	7905	0.0	37.400383	4
AGGGATT	8395	0.0	37.388325	6
AAGGGAC	5945	0.0	37.27923	5
TCACGAC	510	0.0	37.058823	25
TAGGGAC	5490	0.0	37.008198	5
GACCGAT	2585	0.0	36.992264	9
CGGTCTA	505	0.0	36.980194	31
TGGGCGA	1775	0.0	36.887325	6
GGACCGA	2730	0.0	36.758244	8
TATGGGA	7060	0.0	36.713882	4
AAGGGAT	8965	0.0	36.44172	5
>>END_MODULE