FastQCFastQC Report
Thu 26 May 2016
SRR1547455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547455_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4844969
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253560.5233470018074419No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT144300.039.7921031
AGGGCGA41250.039.66
CGTAAGG7250.039.413792
TAAGGGA90300.039.2441834
TACGGGA25500.038.5588264
TTAGGGA92100.038.355054
GGGCGAT86550.038.1889087
AAGGGAT96400.037.6011435
CTCACGA6600.037.524
TAGGGAG95250.037.2283485
CGACGGT6550.037.09923628
TAGGGAC61700.037.0502435
TATGGGA82000.036.9329264
GTAGGGA85050.036.7989434
TAGGGAT90450.036.641795
CGTTAGG6450.036.6279072
CGGTCTA6650.036.54135531
GCGTAAG4250.036.529411
AAGGGAC59550.036.385395
TGGGCGA27800.036.258996
Produced by FastQC (version 0.11.5)