##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547455_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4844969
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265729460807695	31.0	31.0	33.0	30.0	34.0
2	31.678915386249116	31.0	31.0	34.0	30.0	34.0
3	31.82693057478799	31.0	31.0	34.0	30.0	34.0
4	35.576322366562096	37.0	35.0	37.0	33.0	37.0
5	35.44198838011141	37.0	35.0	37.0	33.0	37.0
6	35.48869002051406	37.0	35.0	37.0	33.0	37.0
7	35.91157817521639	37.0	35.0	37.0	35.0	37.0
8	35.94245659776151	37.0	35.0	37.0	35.0	37.0
9	37.66973410975385	39.0	37.0	39.0	35.0	39.0
10	36.98385686265485	39.0	37.0	39.0	33.0	39.0
11	36.79412128333535	39.0	37.0	39.0	32.0	39.0
12	36.40771096781011	39.0	35.0	39.0	32.0	39.0
13	36.29537588372598	39.0	35.0	39.0	32.0	39.0
14	37.34386164287119	39.0	36.0	41.0	32.0	41.0
15	37.46425931724228	39.0	36.0	41.0	32.0	41.0
16	37.51137024818941	39.0	36.0	41.0	32.0	41.0
17	37.44521254934758	39.0	36.0	41.0	32.0	41.0
18	37.355541387364916	39.0	36.0	41.0	32.0	41.0
19	37.29375461432261	39.0	36.0	41.0	32.0	41.0
20	37.179223644155414	39.0	35.0	41.0	31.0	41.0
21	37.11647669985092	39.0	35.0	41.0	31.0	41.0
22	37.06361175891941	39.0	35.0	41.0	31.0	41.0
23	36.96758348711829	39.0	35.0	41.0	31.0	41.0
24	36.88938608275925	39.0	35.0	41.0	31.0	41.0
25	36.7931966128163	39.0	35.0	41.0	31.0	41.0
26	36.6407727273384	38.0	35.0	40.0	31.0	41.0
27	36.541145464501426	38.0	35.0	40.0	30.0	41.0
28	36.423302811638216	38.0	35.0	40.0	30.0	41.0
29	36.47850543522569	38.0	35.0	40.0	30.0	41.0
30	36.438671537423666	38.0	35.0	40.0	30.0	41.0
31	36.337901646016725	38.0	35.0	40.0	30.0	41.0
32	36.29097172757968	38.0	35.0	41.0	30.0	41.0
33	36.21916094819183	38.0	35.0	41.0	30.0	41.0
34	36.066078854168104	38.0	35.0	41.0	29.0	41.0
35	35.95842739138269	38.0	35.0	41.0	29.0	41.0
36	35.90710755837654	38.0	35.0	41.0	29.0	41.0
37	35.86235206045694	38.0	35.0	41.0	28.0	41.0
38	35.748074342684134	38.0	35.0	41.0	27.0	41.0
39	35.66722779856796	38.0	35.0	40.0	27.0	41.0
40	35.55992866001826	38.0	34.0	40.0	27.0	41.0
41	35.45109968711874	38.0	34.0	40.0	26.0	41.0
42	35.413119258348196	38.0	34.0	40.0	26.0	41.0
43	35.376808809303014	38.0	34.0	40.0	26.0	41.0
44	35.268501614767814	38.0	34.0	40.0	26.0	41.0
45	35.14483890402601	38.0	34.0	40.0	26.0	41.0
46	35.112237457040486	38.0	34.0	40.0	26.0	41.0
47	35.05641811124075	38.0	34.0	40.0	26.0	41.0
48	34.96393103856805	38.0	34.0	40.0	26.0	41.0
49	34.874726546237966	37.0	34.0	40.0	26.0	41.0
50	34.766749591173856	37.0	34.0	40.0	25.0	41.0
51	34.200719550527566	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	2.0
11	7.0
12	10.0
13	13.0
14	55.0
15	99.0
16	345.0
17	904.0
18	1927.0
19	3735.0
20	6325.0
21	10540.0
22	16287.0
23	23739.0
24	34106.0
25	48796.0
26	64525.0
27	78138.0
28	89962.0
29	104568.0
30	123966.0
31	149934.0
32	182448.0
33	230331.0
34	334398.0
35	424920.0
36	392534.0
37	503337.0
38	743356.0
39	1275043.0
40	617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.94514371505783	24.781314390246873	28.8985337161084	13.3750081785869
2	30.858381137216774	25.766191692867384	30.066198565976375	13.309228603939468
3	28.711886495042588	25.910423781865273	31.42176967489369	13.95592004819845
4	25.822745202291287	28.516116408587962	30.991529563966253	14.669608825154507
5	23.200210362543082	32.594305556960215	29.605741543444342	14.599742537052352
6	22.406644913517507	41.32639445164665	26.180415189447032	10.086545445388815
7	90.32515997522378	2.850296874964525	5.506763820367065	1.3177793294446258
8	90.40208513202045	2.523153398917516	5.2764630692167485	1.7982983998452828
9	84.15463545793585	6.158553336460977	7.103905102385588	2.582906103217585
10	30.596232917073362	44.679604761145015	12.76839955013128	11.955762771650345
11	33.04592867364063	23.995695328494364	26.231127588226055	16.727248409638946
12	31.108867776037368	22.616491457427284	27.037799416260455	19.236841350274894
13	26.085099822104123	25.602970834281912	29.33886677087098	18.973062572742982
14	21.223789047979462	27.851819072526574	30.39794475465168	20.526447124842285
15	19.856700837507937	26.015316093869746	34.76738447655702	19.360598592065294
16	24.636504382174582	25.34464100802296	31.49326652038434	18.525588089418115
17	23.866489135431003	25.142534451716823	28.314856090926483	22.67612032192569
18	23.99274381322151	24.812996739504424	30.717389523028938	20.47686992424513
19	25.605241230645643	26.563596175744365	27.83386230128614	19.997300292323853
20	26.34693844274339	26.828056072185397	28.208353861500456	18.61665162357076
21	25.823426321200404	25.910939781038845	29.880232463819684	18.385401433941063
22	25.192565731586726	23.43874646050367	29.66235697276907	21.706330835140534
23	22.128191119489102	26.247804681516023	29.80442186523794	21.819582333756934
24	21.414584902400822	26.158144665115508	31.643711239432076	20.783559193051595
25	23.07009601093423	27.057758264294364	29.28437725814138	20.587768466630024
26	21.688683663404245	29.414677369452725	27.322362640503993	21.574276326639037
27	21.239805662327253	27.912913374677938	29.855959862694686	20.991321100300127
28	20.00252220396044	27.85456418813	31.05225234671264	21.090661261196924
29	21.544286454670814	26.347908521189712	30.738277169575284	21.36952785456419
30	23.07857903734781	26.3979810809935	29.538764850714216	20.98467503094447
31	24.214582177925184	26.608302344142963	28.536900855299592	20.64021462263226
32	25.033947585629544	26.861575378500874	28.34187380765491	19.76260322821467
33	25.22245240372023	26.61829208814339	27.53274582355429	20.62650968458209
34	22.48115519418184	27.114229213850493	29.39094140746824	21.013674184499425
35	22.82955783618017	27.794976603565473	28.81157753537742	20.56388802487694
36	24.9699843280731	27.599908275986905	26.9657246516954	20.464382744244595
37	22.040718939584547	29.54964211329319	28.18226494328447	20.227374003837795
38	22.571620169293137	28.892362365992437	27.675966554171964	20.860050910542462
39	22.478471998479247	28.051923552039238	27.551961632778248	21.917642816703264
40	24.336007103451024	26.71571273211449	28.044947243212498	20.903332921221992
41	21.12471720665292	26.26155089950008	29.11446905026637	23.499262843580627
42	22.35700579301952	27.208368103077646	28.12818822989373	22.3064378740091
43	21.87646608265192	26.800687476018936	29.060949615983095	22.261896825346046
44	22.07987295687547	27.432497504111996	28.828192708766558	21.659436830245973
45	21.75270884086152	28.25153267234527	27.29813131931288	22.697627167480327
46	22.458760830048654	27.737184696124988	27.795884762110965	22.00816971171539
47	21.503563799892216	27.383188623085104	28.997357877831625	22.115889699191058
48	21.795681252036907	27.260401459741026	29.671913277463695	21.272004010758376
49	22.60720347230292	26.654845469599497	29.164459050202385	21.5734920078952
50	20.96246642651377	28.64088500875857	28.567943365581904	21.828705199145755
51	20.926779923669276	28.002078031871825	28.080695665957823	22.99044637850108
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2318.0
1	4449.5
2	6581.0
3	23672.0
4	40763.0
5	28396.5
6	16030.0
7	16428.5
8	16827.0
9	17941.0
10	19055.0
11	19507.0
12	19959.0
13	19863.5
14	19768.0
15	19274.5
16	18781.0
17	18568.5
18	18356.0
19	20114.0
20	21872.0
21	22408.0
22	22944.0
23	24371.0
24	25798.0
25	29603.5
26	42233.5
27	51058.0
28	58463.0
29	65868.0
30	78863.0
31	91858.0
32	107298.0
33	122738.0
34	138845.0
35	154952.0
36	172371.5
37	189791.0
38	202024.5
39	214258.0
40	241043.5
41	267829.0
42	291757.0
43	315685.0
44	332182.5
45	348680.0
46	366823.5
47	384967.0
48	393294.0
49	401621.0
50	386368.5
51	371116.0
52	347755.0
53	324394.0
54	304141.5
55	283889.0
56	261945.0
57	240001.0
58	221362.5
59	202724.0
60	182465.5
61	162207.0
62	140788.0
63	119369.0
64	102566.0
65	85763.0
66	73491.0
67	61219.0
68	50509.5
69	39800.0
70	31922.5
71	24045.0
72	19783.5
73	15522.0
74	13076.0
75	8409.0
76	6188.0
77	5075.0
78	3962.0
79	2647.5
80	1333.0
81	1008.5
82	684.0
83	441.5
84	199.0
85	153.0
86	107.0
87	62.0
88	17.0
89	11.5
90	6.0
91	8.0
92	10.0
93	11.0
94	12.0
95	7.0
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4844969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.620562533126922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17037661217788	12.992356327207844
2	8.6194236358872	2.8651933907955085
3	2.9524643652572675	1.472148558287619
4	1.4779218416279167	0.9825556955140357
5	0.8841371400760297	0.7347428312246824
6	0.5879701427859364	0.5863436715471132
7	0.4171879662928467	0.4853729077286814
8	0.35178518620398896	0.4677494148424877
9	0.2741599582751925	0.41010234575330745
>10	2.993662673465197	12.04608837472991
>50	1.5071140498671443	18.34640516451494
>100	1.7580271237599434	47.035546215889696
>500	0.003386330798597526	0.364482743735954
>1k	0.0022575538657316654	0.6823550548157071
>5k	0.0	0.0
>10k+	1.2541965920731472E-4	0.5285573034125701
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25356	0.5233470018074419	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04322009077870261	0.0
2	0.0	0.0	0.0	0.13060971081548717	0.0
3	0.0	0.0	0.0	0.20090943822344373	0.0
4	0.0	0.0	0.0	0.36322213826342337	0.0
5	0.0	0.0	0.0	0.5882803378102109	0.0
6	0.0	0.0	0.0	0.9831848253311838	0.0
7	0.0	0.0	0.0	1.1575719060328353	0.0
8	0.0	0.0	0.0	1.6473996015247983	0.0
9	0.0	0.0	0.0	1.8510541553516648	0.0
10	0.0	0.0	0.0	2.207630224259433	0.0
11	0.0	0.0	0.0	2.5774158720107394	0.0
12	0.0	0.0	0.0	2.894507684156493	0.0
13	0.0	0.0	0.0	3.047098959766306	0.0
14	0.0	0.0	0.0	3.124829075273753	0.0
15	0.0	0.0	0.0	3.2175644467487823	0.0
16	0.0	0.0	0.0	3.383530420937678	0.0
17	0.0	0.0	0.0	3.5763902720533403	0.0
18	0.0	0.0	0.0	3.7904060892856073	0.0
19	0.0	0.0	0.0	3.924338834778922	0.0
20	0.0	0.0	0.0	4.080665944405424	0.0
21	0.0	0.0	0.0	4.275342112612073	0.0
22	0.0	0.0	0.0	4.489048330340194	0.0
23	0.0	0.0	0.0	4.707109581093295	0.0
24	0.0	0.0	0.0	4.873116835216076	0.0
25	0.0	0.0	0.0	5.02857706623097	0.0
26	0.0	0.0	0.0	5.177349947956324	0.0
27	0.0	0.0	0.0	5.342077524128637	0.0
28	0.0	0.0	0.0	5.502388147375143	0.0
29	0.0	0.0	0.0	5.688209769763232	0.0
30	0.0	0.0	0.0	5.935125694302688	0.0
31	0.0	0.0	0.0	6.13547785341867	0.0
32	0.0	0.0	0.0	6.318244760699192	0.0
33	0.0	0.0	0.0	6.505882700178267	0.0
34	0.0	0.0	0.0	6.718598199493123	0.0
35	0.0	0.0	0.0	6.948135271866549	0.0
36	0.0	0.0	0.0	7.144710317031956	0.0
37	0.0	0.0	0.0	7.3576528559831855	0.0
38	0.0	0.0	0.0	7.60122510587787	0.0
39	0.0	0.0	0.0	7.986820968307537	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14430	0.0	39.792103	1
AGGGCGA	4125	0.0	39.6	6
CGTAAGG	725	0.0	39.41379	2
TAAGGGA	9030	0.0	39.244183	4
TACGGGA	2550	0.0	38.558826	4
TTAGGGA	9210	0.0	38.35505	4
GGGCGAT	8655	0.0	38.188908	7
AAGGGAT	9640	0.0	37.601143	5
CTCACGA	660	0.0	37.5	24
TAGGGAG	9525	0.0	37.228348	5
CGACGGT	655	0.0	37.099236	28
TAGGGAC	6170	0.0	37.050243	5
TATGGGA	8200	0.0	36.932926	4
GTAGGGA	8505	0.0	36.798943	4
TAGGGAT	9045	0.0	36.64179	5
CGTTAGG	645	0.0	36.627907	2
CGGTCTA	665	0.0	36.541355	31
GCGTAAG	425	0.0	36.52941	1
AAGGGAC	5955	0.0	36.38539	5
TGGGCGA	2780	0.0	36.25899	6
>>END_MODULE