FastQCFastQC Report
Thu 26 May 2016
SRR1547454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547454_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3736820
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145750.39003751853179974No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114100.041.1546861
CGGTCTA9050.040.77348331
CGACGGT9000.040.7528
CTCACGA9000.040.2524
ACGGTCT9200.039.86413230
CACGACG9200.039.37500426
CGTTAGG5400.038.752
TAGGGAT75200.038.447475
TCTCACG9500.038.3684223
TAGGGAC55200.038.2336965
GACCGAT23350.038.1584639
ATAGGGA76950.038.0409364
TAAGGGA69450.037.775384
TCACGAC9700.037.34536425
GGGCGAT65450.037.127587
GGACCGA26050.037.053748
CGAGGGA34800.036.982764
AACGGGA20800.036.8870164
GTAGGGA75200.036.7719424
AGGGCGA32450.036.7488446
Produced by FastQC (version 0.11.5)