##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547454_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3736820
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226677763445924	31.0	31.0	33.0	30.0	34.0
2	31.644279092918577	31.0	31.0	34.0	30.0	34.0
3	31.800029436793853	31.0	31.0	34.0	30.0	34.0
4	35.55222729486569	37.0	35.0	37.0	33.0	37.0
5	35.42112651933997	37.0	35.0	37.0	33.0	37.0
6	35.464660593766894	37.0	35.0	37.0	33.0	37.0
7	35.88585187405334	37.0	35.0	37.0	35.0	37.0
8	35.92144791560739	37.0	35.0	37.0	35.0	37.0
9	37.653931150015254	39.0	37.0	39.0	35.0	39.0
10	36.92344694151712	39.0	37.0	39.0	32.0	39.0
11	36.77430248178933	39.0	37.0	39.0	32.0	39.0
12	36.4902823791352	39.0	35.0	39.0	32.0	39.0
13	36.43537018106304	39.0	35.0	39.0	32.0	39.0
14	37.5659135307561	40.0	36.0	41.0	32.0	41.0
15	37.63171011715844	40.0	36.0	41.0	32.0	41.0
16	37.638502523536054	40.0	36.0	41.0	32.0	41.0
17	37.55384872699247	39.0	36.0	41.0	32.0	41.0
18	37.448475174078496	39.0	36.0	41.0	32.0	41.0
19	37.40558442740084	39.0	36.0	41.0	32.0	41.0
20	37.307326817989626	39.0	35.0	41.0	32.0	41.0
21	37.25419608115992	39.0	35.0	41.0	32.0	41.0
22	37.1643919161212	39.0	35.0	41.0	31.0	41.0
23	37.048245299479234	39.0	35.0	41.0	31.0	41.0
24	36.9794311740999	39.0	35.0	41.0	31.0	41.0
25	36.880345587959816	39.0	35.0	41.0	31.0	41.0
26	36.74819338367917	39.0	35.0	41.0	31.0	41.0
27	36.66656167543526	39.0	35.0	40.0	30.0	41.0
28	36.55357710566739	38.0	35.0	40.0	30.0	41.0
29	36.59414100759469	38.0	35.0	40.0	30.0	41.0
30	36.54348938402171	38.0	35.0	40.0	30.0	41.0
31	36.491381709581944	38.0	35.0	40.0	30.0	41.0
32	36.447661648139324	39.0	35.0	41.0	30.0	41.0
33	36.366051080865546	39.0	35.0	41.0	30.0	41.0
34	36.241178060489936	39.0	35.0	41.0	30.0	41.0
35	36.130147826226576	39.0	35.0	41.0	29.0	41.0
36	36.09227096836347	39.0	35.0	41.0	29.0	41.0
37	36.0583041195455	39.0	35.0	41.0	29.0	41.0
38	35.949478701141615	39.0	35.0	41.0	28.0	41.0
39	35.861641984361036	38.0	35.0	41.0	28.0	41.0
40	35.776469029816795	38.0	35.0	41.0	27.0	41.0
41	35.674052001434376	38.0	35.0	40.0	27.0	41.0
42	35.62992062769949	38.0	34.0	40.0	27.0	41.0
43	35.594794504418196	38.0	34.0	40.0	27.0	41.0
44	35.46788847201631	38.0	34.0	40.0	27.0	41.0
45	35.343639511670354	38.0	34.0	40.0	26.0	41.0
46	35.2905764794665	38.0	34.0	40.0	26.0	41.0
47	35.22951841405259	38.0	34.0	40.0	26.0	41.0
48	35.14240022264921	38.0	34.0	40.0	26.0	41.0
49	35.055559272322455	38.0	34.0	40.0	26.0	41.0
50	34.966088813483125	38.0	34.0	40.0	26.0	41.0
51	34.376246915826826	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	5.0
12	3.0
13	7.0
14	21.0
15	78.0
16	216.0
17	619.0
18	1326.0
19	2589.0
20	4568.0
21	7249.0
22	11431.0
23	17321.0
24	25285.0
25	36678.0
26	49111.0
27	58783.0
28	66616.0
29	77743.0
30	93015.0
31	112216.0
32	136409.0
33	170288.0
34	240471.0
35	307493.0
36	306824.0
37	398370.0
38	592089.0
39	1019535.0
40	457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.54483223703577	23.601538206282346	28.792850605595135	14.060778951086753
2	30.71724086255158	26.201877532233294	29.63265557345551	13.448226031759624
3	28.598246637515324	25.870660079961038	32.018507715116066	13.512585567407582
4	25.623310729443748	28.79394779518414	30.771832734785193	14.810908740586918
5	23.10831134494035	32.21019476453241	30.020980405799584	14.660513484727655
6	21.41470555177932	41.28100898625034	26.67048452962679	10.633800932343544
7	90.4762338030732	2.5144641700697385	5.710470399965746	1.298831626891314
8	90.91505611723338	2.1653973164348295	5.139236034917389	1.7803105314144112
9	84.87398376159409	5.690159012208242	7.0444656151487095	2.3913916110489666
10	27.306613644756773	45.792251165429434	14.042822506837366	12.858312682976432
11	29.94176866961748	25.81028789184387	27.16815367076819	17.079789767770457
12	28.936555681033603	23.71816143137748	27.370598530301166	19.974684357287746
13	24.77727051343121	25.687215332823094	29.391407667481978	20.14410648626372
14	21.270331458298767	27.991099383968187	29.461922169117056	21.276646988615987
15	20.297284857178028	26.992603336526777	32.51261768027361	20.19749412602159
16	23.308133653748374	26.556189487318093	31.366241884811146	18.769434974122383
17	23.238850145310717	26.743648342708504	28.518633490507973	21.498868021472802
18	22.751002189027034	26.378792663280542	30.51412698497653	20.356078162715892
19	24.334648176792033	27.792160178975706	27.340305393355845	20.532886250876416
20	25.4740126631735	27.444859532971883	27.670398895317412	19.410728908537205
21	25.175229205581218	27.19360311708886	28.826408550585793	18.80475912674413
22	24.729716711000265	24.51303514753186	28.59516915452176	22.16207898694612
23	21.61688280409546	28.066966029939895	28.988337677490488	21.327813488474156
24	20.950112662638286	26.822485428787047	31.90967186003072	20.31773004854395
25	21.928431125930604	28.28511943310087	28.869332748165554	20.917116692802974
26	21.40354633083745	29.519725327952646	27.81857301127697	21.258155329932936
27	20.5492638125465	28.62412425538292	29.021975904646197	21.804636027424387
28	20.06484122863825	28.184258273077106	30.684726585706564	21.066173912578073
29	20.834452823523744	26.104227658811503	30.13193035789789	22.92938915976686
30	21.246862305382653	27.20040034039638	29.747485830197867	21.8052515240231
31	22.6672411301588	27.489683741791147	28.07740271139632	21.765672416653732
32	22.995648706654322	26.736182101358906	28.197183701650065	22.070985490336703
33	22.456179318243855	26.661064755594328	27.8837621292971	22.998993796864713
34	20.50484101455248	27.1251224303017	29.83485958649333	22.53517696865249
35	21.04230334883671	27.04955015226851	29.476426480269318	22.431720018625462
36	22.05562483609058	28.03881375072923	28.472952938594847	21.432608474585344
37	21.25510460766106	28.97107701200486	28.21468521363084	21.55913316670324
38	21.23985099630167	28.672025947195745	29.095112957006226	20.993010099496363
39	21.181512623032418	28.59158321781622	28.38983413704701	21.837070022104356
40	23.007022013369657	27.102723706252913	29.062438115831107	20.827816164546327
41	20.02336211002938	27.172087496855614	29.762766202278947	23.041784190836058
42	21.872982910603135	27.4873823197264	27.83492381222537	22.804710957445103
43	21.383903961122023	27.072965783741253	29.000192677196118	22.542937577940602
44	21.205811358320712	27.30061924309975	28.5546266611718	22.938942737407743
45	21.117902387591588	27.66063123190306	28.3673016093898	22.85416477111555
46	21.56263882124373	27.970600671158902	28.118721265675095	22.348039241922276
47	21.23176925835336	27.331741962417244	29.02516043052649	22.41132834870291
48	20.897126433705665	27.20516374885598	29.87898801654883	22.018721800889526
49	21.049234375752647	27.009596394795576	29.182326148971587	22.758843080480197
50	21.034382175218504	27.672860881712257	29.65703459090885	21.635722352160393
51	20.21376464480494	27.096649022430835	29.596314513409798	23.093271819354424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1433.0
1	2421.0
2	3409.0
3	15206.5
4	27004.0
5	19639.0
6	12274.0
7	12399.5
8	12525.0
9	13290.0
10	14055.0
11	14450.0
12	14845.0
13	14883.5
14	14922.0
15	14496.5
16	14071.0
17	13769.5
18	13468.0
19	13668.5
20	13869.0
21	16102.0
22	18335.0
23	18637.5
24	18940.0
25	23252.5
26	35634.0
27	43703.0
28	50678.0
29	57653.0
30	68478.0
31	79303.0
32	87528.0
33	95753.0
34	114268.0
35	132783.0
36	144194.5
37	155606.0
38	167426.5
39	179247.0
40	196360.0
41	213473.0
42	229342.5
43	245212.0
44	257391.5
45	269571.0
46	288288.0
47	307005.0
48	312689.0
49	318373.0
50	312037.5
51	305702.0
52	280677.5
53	255653.0
54	231994.0
55	208335.0
56	189953.5
57	171572.0
58	155250.5
59	138929.0
60	124093.0
61	109257.0
62	93352.0
63	77447.0
64	68154.0
65	58861.0
66	48511.5
67	38162.0
68	30599.5
69	23037.0
70	19798.5
71	16560.0
72	14049.5
73	11539.0
74	9764.0
75	6521.0
76	5053.0
77	3609.5
78	2166.0
79	1629.5
80	1093.0
81	899.5
82	706.0
83	423.5
84	141.0
85	112.0
86	83.0
87	94.0
88	105.0
89	64.5
90	24.0
91	14.5
92	5.0
93	3.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3736820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.097437670770937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.36139608036862	14.00043043688294
2	7.701896182179587	2.787691722074874
3	2.6242450362894654	1.4247633293123585
4	1.3599749846071247	0.984482500709404
5	0.8987868994612834	0.813286994615302
6	0.6127576010266781	0.6653605495122858
7	0.5054945053089647	0.6403708711922322
8	0.3978619588309475	0.5760225601211124
9	0.33019413699966704	0.5378101032244927
>10	4.933464084888758	22.210403689246032
>50	2.21066459622003	28.8162686390688
>100	1.0589247088129983	25.399430620510806
>500	0.0026933120726351733	0.33117513291264594
>1k	0.0014962844847973187	0.4178276615570014
>5k	0.0	0.0
>10k+	1.4962844847973188E-4	0.3946751890598377
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14575	0.39003751853179974	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6760721683142352E-5	0.0	0.0	0.04313828335322547	0.0
2	2.6760721683142352E-5	0.0	0.0	0.1308599290305661	0.0
3	2.6760721683142352E-5	0.0	0.0	0.21625339192147333	0.0
4	2.6760721683142352E-5	0.0	0.0	0.39959109617268157	0.0
5	2.6760721683142352E-5	0.0	0.0	0.6855026466353744	0.0
6	2.6760721683142352E-5	0.0	0.0	1.2884217061565717	0.0
7	2.6760721683142352E-5	0.0	0.0	1.5444415304991945	0.0
8	2.6760721683142352E-5	0.0	0.0	2.251004865099202	0.0
9	2.6760721683142352E-5	0.0	0.0	2.5368628941185287	0.0
10	2.6760721683142352E-5	0.0	0.0	3.030009473295476	0.0
11	2.6760721683142352E-5	0.0	0.0	3.471240252407127	0.0
12	2.6760721683142352E-5	0.0	0.0	3.830663505333412	0.0
13	2.6760721683142352E-5	0.0	0.0	4.012663173500463	0.0
14	2.6760721683142352E-5	0.0	0.0	4.099073543815329	0.0
15	5.3521443366284704E-5	0.0	0.0	4.190729015580092	0.0
16	5.3521443366284704E-5	0.0	0.0	4.3584384583683455	0.0
17	5.3521443366284704E-5	0.0	0.0	4.545415620768461	0.0
18	5.3521443366284704E-5	0.0	0.0	4.765629599499039	0.0
19	5.3521443366284704E-5	0.0	0.0	4.896569810694655	0.0
20	5.3521443366284704E-5	0.0	0.0	5.04343265129174	0.0
21	5.3521443366284704E-5	0.0	0.0	5.236163368853731	0.0
22	5.3521443366284704E-5	0.0	0.0	5.4484561739660995	0.0
23	5.3521443366284704E-5	0.0	0.0	5.670623685379548	0.0
24	5.3521443366284704E-5	0.0	0.0	5.851552924679273	0.0
25	5.3521443366284704E-5	0.0	0.0	6.008558078794269	0.0
26	5.3521443366284704E-5	0.0	0.0	6.151112443200368	0.0
27	5.3521443366284704E-5	0.0	0.0	6.29896543049973	0.0
28	5.3521443366284704E-5	0.0	0.0	6.454605787808886	0.0
29	5.3521443366284704E-5	0.0	0.0	6.62266311997902	0.0
30	5.3521443366284704E-5	0.0	0.0	6.824465722191596	0.0
31	8.028216504942705E-5	0.0	0.0	7.002478042827859	0.0
32	8.028216504942705E-5	0.0	0.0	7.167645217056213	0.0
33	8.028216504942705E-5	0.0	0.0	7.339475810983671	0.0
34	8.028216504942705E-5	0.0	0.0	7.521930411419335	0.0
35	8.028216504942705E-5	0.0	0.0	7.711316038770933	0.0
36	8.028216504942705E-5	0.0	0.0	7.887615673219475	0.0
37	8.028216504942705E-5	0.0	0.0	8.066778704888113	0.0
38	8.028216504942705E-5	0.0	0.0	8.262158733896737	0.0
39	8.028216504942705E-5	0.0	0.0	8.476003660866727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11410	0.0	41.154686	1
CGGTCTA	905	0.0	40.773483	31
CGACGGT	900	0.0	40.75	28
CTCACGA	900	0.0	40.25	24
ACGGTCT	920	0.0	39.864132	30
CACGACG	920	0.0	39.375004	26
CGTTAGG	540	0.0	38.75	2
TAGGGAT	7520	0.0	38.44747	5
TCTCACG	950	0.0	38.36842	23
TAGGGAC	5520	0.0	38.233696	5
GACCGAT	2335	0.0	38.158463	9
ATAGGGA	7695	0.0	38.040936	4
TAAGGGA	6945	0.0	37.77538	4
TCACGAC	970	0.0	37.345364	25
GGGCGAT	6545	0.0	37.12758	7
GGACCGA	2605	0.0	37.05374	8
CGAGGGA	3480	0.0	36.98276	4
AACGGGA	2080	0.0	36.887016	4
GTAGGGA	7520	0.0	36.771942	4
AGGGCGA	3245	0.0	36.748844	6
>>END_MODULE