FastQCFastQC Report
Thu 26 May 2016
SRR1547453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547453_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2516035
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172230.6845294282472223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAT351.212702E-745.00000426
TAACGCG253.8921324E-545.0000041
TATAGCG1150.045.0000041
CTTACGA207.0348877E-445.016
TCGTTAG1250.043.1999971
TAATGCG850.042.352941
CGTTTTT97400.042.343431
CGCGTAA800.042.187531
CGACGGT5250.042.028
CGTAAGG3700.041.9594572
TCACGAC5500.040.525
GCGTAAG1900.040.263161
CACGACG5650.039.4247826
ACGGGTA1600.039.3755
TACGGGA14800.039.0709464
CACGACC6750.039.027
GGCGATA10400.038.9423078
TAAGGGA39600.038.754
GGGCGAT39900.038.4586457
TATGGGA38600.038.413214
Produced by FastQC (version 0.11.5)