FastQCFastQC Report
Thu 26 May 2016
SRR1547452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547452_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3904976
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373310.955985388898677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT182950.042.7985761
CGACGGT3600.040.62500428
CGCGTAA403.461064E-739.37531
TAATGCG1500.039.01
TTAGGGA64000.038.7773444
TAGGGAC51000.038.5147065
CGGTCTA3900.038.07692331
TACGGGA19800.037.9545444
CGTTAGG6300.037.8571432
GGGCGAT68900.037.8156787
AGGGCGA37000.037.70276
CGACAAT2150.037.6744220
TAAGGGA64450.037.494184
TAGGGCG12250.037.4693875
ACGGGAC18450.037.195125
AGGGATT73950.037.0588266
ATAGGGA67800.036.935844
TAGGGAG65650.036.877385
TATGGGA59200.036.828554
AAGGGAT78000.036.755
Produced by FastQC (version 0.11.5)