##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547452_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3904976
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238909790994875	31.0	31.0	33.0	30.0	34.0
2	31.647634966258437	31.0	31.0	34.0	30.0	34.0
3	31.75847892535063	31.0	31.0	34.0	30.0	34.0
4	35.53567909252195	37.0	35.0	37.0	33.0	37.0
5	35.415588213602334	37.0	35.0	37.0	33.0	37.0
6	35.460283238616576	37.0	35.0	37.0	33.0	37.0
7	35.908663203051695	37.0	35.0	37.0	35.0	37.0
8	35.95032799177255	37.0	35.0	37.0	35.0	37.0
9	37.68221315572746	39.0	37.0	39.0	35.0	39.0
10	36.963659699829144	39.0	37.0	39.0	32.0	39.0
11	36.71017517137109	39.0	37.0	39.0	32.0	39.0
12	36.44682118404825	39.0	35.0	39.0	32.0	39.0
13	36.39233864689565	39.0	35.0	39.0	32.0	39.0
14	37.474494849648245	40.0	36.0	41.0	32.0	41.0
15	37.5692613731813	40.0	36.0	41.0	32.0	41.0
16	37.58627735484162	40.0	36.0	41.0	32.0	41.0
17	37.50568070072646	39.0	36.0	41.0	32.0	41.0
18	37.39038165663502	39.0	36.0	41.0	32.0	41.0
19	37.30550328606373	39.0	36.0	41.0	32.0	41.0
20	37.17566228319969	39.0	35.0	41.0	32.0	41.0
21	37.13199773827035	39.0	35.0	41.0	31.0	41.0
22	37.05629176722213	39.0	35.0	41.0	31.0	41.0
23	36.966496593064846	39.0	35.0	41.0	31.0	41.0
24	36.884179825945154	39.0	35.0	41.0	31.0	41.0
25	36.7897062619591	38.0	35.0	40.0	31.0	41.0
26	36.6617789712408	38.0	35.0	40.0	31.0	41.0
27	36.568859834221776	38.0	35.0	40.0	30.0	41.0
28	36.45335874023297	38.0	35.0	40.0	30.0	41.0
29	36.4893699730805	38.0	35.0	40.0	30.0	41.0
30	36.42120335694765	38.0	35.0	40.0	30.0	41.0
31	36.32291466067909	38.0	35.0	40.0	30.0	41.0
32	36.22926107612441	38.0	35.0	40.0	30.0	41.0
33	36.084909868844264	38.0	35.0	41.0	29.0	41.0
34	35.910729797058934	38.0	35.0	41.0	28.0	41.0
35	35.775311039043515	38.0	35.0	41.0	27.0	41.0
36	35.71810377323702	38.0	35.0	41.0	27.0	41.0
37	35.66855775810146	38.0	35.0	41.0	27.0	41.0
38	35.554969864091355	38.0	34.0	40.0	27.0	41.0
39	35.473834154166376	38.0	34.0	40.0	26.0	41.0
40	35.369518020085145	38.0	34.0	40.0	26.0	41.0
41	35.2707238149479	38.0	34.0	40.0	25.0	41.0
42	35.23367390734283	38.0	34.0	40.0	25.0	41.0
43	35.20728987835008	38.0	34.0	40.0	25.0	41.0
44	35.09424027189924	38.0	34.0	40.0	25.0	41.0
45	34.98925780849869	38.0	34.0	40.0	24.0	41.0
46	34.96350912271932	38.0	34.0	40.0	24.0	41.0
47	34.91568654967406	38.0	34.0	40.0	24.0	41.0
48	34.83333034569227	38.0	34.0	40.0	24.0	41.0
49	34.75831119064496	38.0	34.0	40.0	24.0	41.0
50	34.66683918159804	37.0	33.0	40.0	24.0	41.0
51	34.11426856400654	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	7.0
12	8.0
13	5.0
14	21.0
15	85.0
16	260.0
17	682.0
18	1459.0
19	2949.0
20	5210.0
21	8280.0
22	12991.0
23	19354.0
24	29130.0
25	42992.0
26	58641.0
27	68734.0
28	76858.0
29	86547.0
30	101113.0
31	120591.0
32	146886.0
33	183110.0
34	262260.0
35	334861.0
36	317866.0
37	401708.0
38	594923.0
39	1027000.0
40	437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.460551870229164	25.913245049393392	27.027285186899995	15.598917893477452
2	29.800516059509714	26.87020355566846	30.22945595568321	13.099824429138618
3	27.40713387226964	26.28935491536952	33.40686344807241	12.896647764288435
4	24.256026157395077	28.62076489074453	32.88929304559106	14.233915906269335
5	23.094303268445184	32.53236383527069	30.570866504685306	13.802466391598822
6	21.270681305083567	41.13103383990068	27.178861022449308	10.41942383256645
7	88.70246577699838	2.4593493020187576	7.38785590487624	1.4503290161066291
8	89.0367828124936	2.0765300478159148	6.978352747878604	1.908334391811883
9	83.26896247249664	5.481134839241009	8.889683316875699	2.3602193713866617
10	32.923172895300766	41.47782214282495	14.285849644146339	11.313155317727945
11	27.417377213073777	24.665093972408535	30.276268023158142	17.641260791359535
12	27.86155407869344	22.74159943620652	29.90691876211275	19.489927722987286
13	24.238612478028035	24.564171457135718	31.540091411573336	19.657124653262915
14	19.720966274824736	27.46165405369969	31.70475311499994	21.112626556475636
15	18.885545007190828	26.566104375545457	35.25535112123608	19.292999496027633
16	22.572225796010017	25.399029341025397	33.886533489578426	18.14221137338616
17	22.10489898017299	26.205922904519774	29.97198958457107	21.717188530736166
18	22.05329815087212	25.42112934881034	32.24334797448179	20.282224525835755
19	23.30931099192415	27.41161533387145	29.59785156169974	19.68122211250466
20	24.662149011927344	26.91181712768529	29.80809613170478	18.617937728682584
21	24.023425496084997	27.045723200347453	30.718191353800893	18.212659949766657
22	22.78574823507238	24.099175001331634	31.20918028689549	21.905896476700498
23	20.848169105264667	27.463267379876342	31.145210623573615	20.543352891285373
24	20.522507692748945	26.11614002237146	33.73308824433236	19.62826404054724
25	21.015366035540296	27.910466031033227	30.028327958993856	21.04583997443262
26	20.404427581629182	29.29037207911137	29.542998471693554	20.762201867565896
27	19.85774048291206	28.626296294778765	30.651788897038035	20.864174325271144
28	18.72080647870819	28.80120133900951	32.15464064311791	20.32335153916439
29	19.85630641519948	26.503799255104255	31.765905859600675	21.87398847009559
30	20.755697346155262	28.054154494163345	31.313329454521615	19.876818705159778
31	22.22205719062038	28.26060902807085	29.85885188538931	19.658481895919465
32	23.28864505185179	27.894102294098605	29.532575872425337	19.284676781624267
33	22.980730227279246	28.418356476454658	28.84842314011661	19.752490156149488
34	21.290630211299636	27.720964226156575	30.511531953077302	20.476873609466487
35	21.192601439803983	28.593261520685402	29.720566784533375	20.49357025497724
36	23.04695342557803	28.573824781509536	28.447396347634403	19.931825445278026
37	21.961031258578796	29.788480134064844	28.855516653623482	19.394971953732878
38	22.00192779673934	29.215595691241127	29.09515961173641	19.687316900283125
39	22.089764444134868	28.471903540508315	28.39781857814235	21.040513437214468
40	23.341321431937097	27.16201072682649	29.390423910415837	20.106243930820575
41	20.738642183716365	27.510079447351277	29.40263909432478	22.348639274607578
42	21.315777612973804	27.907136945271876	28.810881296069425	21.96620414568489
43	21.021179131446647	27.4164297040494	29.66937568886467	21.893015475639288
44	21.07572492122871	27.926471251039697	28.94568366105195	22.052120166679643
45	20.413262463072755	28.31745956953385	28.626142644666704	22.643135322726696
46	21.20881152662654	28.572288280388918	28.615668828694467	21.603231364290075
47	20.8504738569456	27.789389742728254	30.127688364794047	21.2324480355321
48	20.838386714796712	27.141626478626247	30.76213528585067	21.257851520726376
49	21.24287063480032	27.00487787889093	29.97905748972593	21.773193996582823
50	20.23472103285654	27.47960550845895	30.663927255890943	21.621746202793563
51	19.911364372021747	26.93504390295869	30.002233048295302	23.15135867672426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2007.0
1	3496.5
2	4986.0
3	30935.5
4	56885.0
5	38301.5
6	19718.0
7	19536.5
8	19355.0
9	20180.5
10	21006.0
11	21563.5
12	22121.0
13	22525.0
14	22929.0
15	22216.0
16	21503.0
17	20618.0
18	19733.0
19	19773.5
20	19814.0
21	21295.5
22	22777.0
23	24019.5
24	25262.0
25	28741.5
26	39496.5
27	46772.0
28	57773.5
29	68775.0
30	75278.5
31	81782.0
32	94729.0
33	107676.0
34	120790.5
35	133905.0
36	146009.5
37	158114.0
38	174430.5
39	190747.0
40	207415.5
41	224084.0
42	239361.5
43	254639.0
44	266674.0
45	278709.0
46	299435.0
47	320161.0
48	323855.5
49	327550.0
50	311366.0
51	295182.0
52	270595.0
53	246008.0
54	229562.5
55	213117.0
56	194751.0
57	176385.0
58	160624.5
59	144864.0
60	130370.5
61	115877.0
62	98841.5
63	81806.0
64	67608.5
65	53411.0
66	41984.5
67	30558.0
68	23960.5
69	17363.0
70	13993.5
71	10624.0
72	8871.5
73	7119.0
74	5493.0
75	3443.0
76	3019.0
77	2201.5
78	1384.0
79	972.5
80	561.0
81	481.0
82	401.0
83	246.0
84	91.0
85	68.0
86	45.0
87	40.0
88	35.0
89	22.0
90	9.0
91	11.0
92	13.0
93	9.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3904976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.90961128830783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.82139853460768	15.294841834482199
2	8.675075362034883	3.4543475670978165
3	2.9114120674015056	1.7389524768615796
4	1.5247980912761223	1.2143254916184527
5	0.939682520448703	0.9354356858275527
6	0.6554456508980953	0.7829800877995794
7	0.511174583028778	0.7124101073996063
8	0.3948075528505404	0.6288371928753849
9	0.3438690178376004	0.6161668631314934
>10	4.449483310887665	22.17313112522164
>50	1.8986344094788559	27.105729725348997
>100	0.8695388937689768	23.27534574653328
>500	0.002210002588064208	0.2979078625774356
>1k	0.002340002740303271	0.8033676930096436
>5k	0.0	0.0
>10k+	1.300001522390706E-4	0.9662205402153099
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37331	0.955985388898677	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.68250560310742E-5	0.0	0.0	0.0862233212188756	0.0
2	7.68250560310742E-5	0.0	0.0	0.23895153260865112	0.0
3	7.68250560310742E-5	0.0	0.0	0.3935491536951828	0.0
4	7.68250560310742E-5	0.0	0.0	0.641873343139625	0.0
5	7.68250560310742E-5	0.0	0.0	1.0222598039014836	0.0
6	7.68250560310742E-5	0.0	0.0	1.643569640376791	0.0
7	7.68250560310742E-5	0.0	0.0	1.9325598928136818	0.0
8	7.68250560310742E-5	0.0	0.0	2.659273706163623	0.0
9	7.68250560310742E-5	0.0	0.0	2.954589221547072	0.0
10	7.68250560310742E-5	0.0	0.0	3.4319801197241673	0.0
11	7.68250560310742E-5	0.0	0.0	3.939051097881267	0.0
12	7.68250560310742E-5	0.0	0.0	4.342024125116262	0.0
13	7.68250560310742E-5	0.0	0.0	4.543459422029739	0.0
14	7.68250560310742E-5	0.0	0.0	4.648735357144321	0.0
15	7.68250560310742E-5	0.0	0.0	4.7712201048098635	0.0
16	7.68250560310742E-5	0.0	0.0	4.989710564162238	0.0
17	7.68250560310742E-5	0.0	0.0	5.260057936335588	0.0
18	7.68250560310742E-5	0.0	0.0	5.5536833004863535	0.0
19	7.68250560310742E-5	0.0	0.0	5.743671664051201	0.0
20	7.68250560310742E-5	0.0	0.0	5.932354001663518	0.0
21	7.68250560310742E-5	0.0	0.0	6.178834389763215	0.0
22	7.68250560310742E-5	0.0	0.0	6.462037154645765	0.0
23	7.68250560310742E-5	0.0	0.0	6.749337255849972	0.0
24	7.68250560310742E-5	0.0	0.0	6.975510220805455	0.0
25	7.68250560310742E-5	0.0	0.0	7.175306583190268	0.0
26	7.68250560310742E-5	0.0	0.0	7.363092628482224	0.0
27	7.68250560310742E-5	0.0	0.0	7.561557356562498	0.0
28	7.68250560310742E-5	0.0	0.0	7.767013164741601	0.0
29	7.68250560310742E-5	0.0	0.0	7.983531780989179	0.0
30	7.68250560310742E-5	0.0	0.0	8.254212061738663	0.0
31	7.68250560310742E-5	0.0	0.0	8.477183982692852	0.0
32	7.68250560310742E-5	0.0	0.0	8.699131569566624	0.0
33	7.68250560310742E-5	0.0	0.0	8.921130373144418	0.0
34	7.68250560310742E-5	0.0	0.0	9.152604266966046	0.0
35	7.68250560310742E-5	0.0	0.0	9.406459860444725	0.0
36	7.68250560310742E-5	0.0	0.0	9.642952991260382	0.0
37	7.68250560310742E-5	0.0	0.0	9.892736856769414	0.0
38	7.68250560310742E-5	0.0	0.0	10.160344135277656	0.0
39	7.68250560310742E-5	0.0	0.0	10.48180577806368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18295	0.0	42.798576	1
CGACGGT	360	0.0	40.625004	28
CGCGTAA	40	3.461064E-7	39.375	31
TAATGCG	150	0.0	39.0	1
TTAGGGA	6400	0.0	38.777344	4
TAGGGAC	5100	0.0	38.514706	5
CGGTCTA	390	0.0	38.076923	31
TACGGGA	1980	0.0	37.954544	4
CGTTAGG	630	0.0	37.857143	2
GGGCGAT	6890	0.0	37.815678	7
AGGGCGA	3700	0.0	37.7027	6
CGACAAT	215	0.0	37.67442	20
TAAGGGA	6445	0.0	37.49418	4
TAGGGCG	1225	0.0	37.469387	5
ACGGGAC	1845	0.0	37.19512	5
AGGGATT	7395	0.0	37.058826	6
ATAGGGA	6780	0.0	36.93584	4
TAGGGAG	6565	0.0	36.87738	5
TATGGGA	5920	0.0	36.82855	4
AAGGGAT	7800	0.0	36.75	5
>>END_MODULE