SRR1547451_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1547451_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1295000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7861	0.607027027027027	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4052	0.31289575289575294	No Hit
GAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	2800	0.21621621621621623	Illumina PCR Primer Index 8 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	2092	0.16154440154440153	TruSeq Adapter, Index 19 (95% over 22bp)
AAGAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1839	0.14200772200772202	No Hit
GTCCCAGGGATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGT	1733	0.1338223938223938	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1542	0.11907335907335907	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	1507	0.11637065637065637	RNA PCR Primer, Index 40 (96% over 26bp)
TAGAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1484	0.11459459459459459	RNA PCR Primer, Index 40 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1411	0.10895752895752894	Illumina PCR Primer Index 8 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1377	0.10633204633204633	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	45	3.8562575E-10	45.000004	1
CGTTCGA	45	3.8562575E-10	45.000004	33
AGTCGAT	45	3.8562575E-10	45.000004	42
AACGTTG	20	7.033619E-4	45.0	1
TTTAGCG	70	0.0	45.0	1
AACCGGT	20	7.033619E-4	45.0	45
TCCGCGA	50	2.1827873E-11	45.0	29
GTCGACG	25	3.8910803E-5	45.0	1
TACTTAC	20	7.033619E-4	45.0	44
ACGTTCA	20	7.033619E-4	45.0	15
TAGGTTA	20	7.033619E-4	45.0	38
CAGTTGA	40	6.8157533E-9	45.0	20
CATTGCG	20	7.033619E-4	45.0	1
ATTACCG	20	7.033619E-4	45.0	18
CGCGATA	25	3.8910803E-5	45.0	39
TCGGCCA	20	7.033619E-4	45.0	43
ATCGTCC	25	3.8910803E-5	45.0	37
CTGCGCG	20	7.033619E-4	45.0	1
TAGCACG	20	7.033619E-4	45.0	1
GTTACGA	25	3.8910803E-5	45.0	16