##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547451_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1295000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22446640926641	31.0	31.0	33.0	30.0	34.0
2	31.619524324324324	31.0	31.0	34.0	30.0	34.0
3	31.745342084942084	31.0	31.0	34.0	30.0	34.0
4	35.49972972972973	37.0	35.0	37.0	33.0	37.0
5	35.37878378378378	37.0	35.0	37.0	33.0	37.0
6	35.416718146718146	37.0	35.0	37.0	33.0	37.0
7	35.841160617760615	37.0	35.0	37.0	35.0	37.0
8	35.86375984555985	37.0	35.0	37.0	35.0	37.0
9	37.606742857142855	39.0	37.0	39.0	35.0	39.0
10	36.969566023166024	39.0	37.0	39.0	32.0	39.0
11	36.75008803088803	39.0	35.0	39.0	32.0	39.0
12	36.02930965250965	38.0	35.0	39.0	32.0	39.0
13	35.68787953667954	38.0	35.0	39.0	30.0	39.0
14	36.75275135135135	39.0	35.0	41.0	31.0	41.0
15	37.00075907335907	39.0	35.0	41.0	32.0	41.0
16	37.11723783783784	39.0	35.0	41.0	32.0	41.0
17	37.041153667953665	38.0	35.0	41.0	32.0	41.0
18	36.94821698841699	38.0	35.0	40.0	32.0	41.0
19	36.82147644787645	38.0	35.0	40.0	31.0	41.0
20	36.66834362934363	38.0	35.0	40.0	31.0	41.0
21	36.58063166023166	38.0	35.0	40.0	31.0	41.0
22	36.46973204633205	38.0	35.0	40.0	31.0	41.0
23	36.39548185328185	38.0	35.0	40.0	31.0	41.0
24	36.30898841698842	38.0	35.0	40.0	31.0	41.0
25	36.18620694980695	38.0	35.0	40.0	31.0	41.0
26	36.01396833976834	37.0	35.0	40.0	30.0	41.0
27	35.898714285714284	37.0	34.0	40.0	30.0	41.0
28	35.8796138996139	37.0	35.0	40.0	30.0	41.0
29	35.892785328185326	37.0	35.0	40.0	30.0	41.0
30	35.80262934362934	37.0	35.0	40.0	30.0	41.0
31	35.634816988416986	37.0	34.0	40.0	30.0	41.0
32	35.40034671814672	37.0	34.0	40.0	29.0	41.0
33	35.22793204633204	37.0	34.0	40.0	27.0	41.0
34	35.07004633204633	37.0	34.0	40.0	26.0	41.0
35	34.94765945945946	37.0	34.0	40.0	25.0	41.0
36	34.81305868725869	37.0	34.0	40.0	24.0	41.0
37	34.72166254826255	37.0	34.0	40.0	24.0	41.0
38	34.582992277992275	37.0	33.0	40.0	24.0	41.0
39	34.46970810810811	37.0	33.0	40.0	23.0	41.0
40	34.27657992277992	37.0	33.0	40.0	23.0	41.0
41	34.19006023166023	36.0	33.0	40.0	23.0	41.0
42	34.10251583011583	36.0	33.0	40.0	22.0	41.0
43	34.02864864864865	36.0	33.0	40.0	22.0	41.0
44	33.85429111969112	36.0	33.0	40.0	21.0	41.0
45	33.72235444015444	36.0	33.0	40.0	20.0	41.0
46	33.65908725868726	36.0	33.0	40.0	20.0	41.0
47	33.629219305019305	35.0	33.0	40.0	20.0	41.0
48	33.557633204633206	35.0	33.0	40.0	20.0	41.0
49	33.52290193050193	36.0	33.0	40.0	20.0	41.0
50	33.36096370656371	35.0	33.0	40.0	20.0	41.0
51	32.81117837837838	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	11.0
15	55.0
16	132.0
17	375.0
18	879.0
19	1583.0
20	2764.0
21	4378.0
22	6529.0
23	9725.0
24	13982.0
25	18994.0
26	23733.0
27	26634.0
28	29199.0
29	33365.0
30	38524.0
31	44452.0
32	53364.0
33	67019.0
34	108090.0
35	164156.0
36	95378.0
37	112824.0
38	160829.0
39	277842.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36239382239382	25.01675675675676	28.615598455598455	14.005250965250966
2	29.0211583011583	28.444169884169884	29.614285714285714	12.9203861003861
3	28.26849420849421	26.50880308880309	31.48285714285714	13.739845559845559
4	24.196525096525097	29.226409266409263	31.129652509652512	15.447413127413128
5	22.867799227799228	33.12131274131274	30.28872586872587	13.722162162162164
6	20.814826254826254	42.2988416988417	26.52247104247104	10.363861003861004
7	85.99250965250965	4.524015444015443	7.7867181467181465	1.696756756756757
8	85.1423166023166	4.383397683397684	6.760772200772201	3.7135135135135133
9	80.56262548262548	7.207181467181467	9.384478764478764	2.8457142857142856
10	37.156138996139	38.25374517374517	12.868648648648648	11.721467181467181
11	37.37289575289575	22.922625482625484	24.375521235521237	15.32895752895753
12	33.869884169884166	22.614903474903475	25.1984555984556	18.316756756756757
13	21.612586872586874	36.964710424710425	25.879691119691117	15.543011583011582
14	16.295135135135137	38.99899613899614	27.244247104247105	17.46162162162162
15	14.747799227799227	26.46849420849421	42.573281853281856	16.21042471042471
16	17.113127413127412	23.7	40.396138996138994	18.79073359073359
17	16.514208494208493	24.648108108108108	27.93969111969112	30.897992277992277
18	19.03760617760618	24.892200772200773	34.58332046332047	21.48687258687259
19	24.848880308880307	27.56030888030888	27.404710424710427	20.186100386100385
20	26.555366795366798	26.416447876447872	26.884710424710423	20.143474903474903
21	21.259305019305017	30.744942084942085	29.063397683397685	18.932355212355212
22	22.28262548262548	26.34996138996139	26.65760617760618	24.70980694980695
23	18.48980694980695	31.02092664092664	25.827799227799225	24.66146718146718
24	18.674517374517375	26.183938223938224	36.90702702702703	18.234517374517374
25	17.848880308880307	27.8196138996139	33.472277992277995	20.8592277992278
26	17.447181467181466	34.95050193050193	26.94262548262548	20.65969111969112
27	16.92826254826255	34.669266409266406	28.28772200772201	20.114749034749035
28	15.692818532818533	31.175984555984552	34.79660231660232	18.334594594594595
29	15.456216216216218	26.659845559845557	33.9619305019305	23.92200772200772
30	17.492818532818532	33.21544401544402	29.63305019305019	19.65868725868726
31	24.68208494208494	30.16965250965251	25.292509652509654	19.855752895752897
32	25.724633204633207	29.796602316602318	26.575444015444017	17.903320463320462
33	25.43714285714286	28.95976833976834	24.05081081081081	21.552277992277993
34	18.277451737451738	30.75119691119691	27.022934362934365	23.948416988416987
35	18.753513513513514	27.485791505791507	29.16532818532819	24.595366795366797
36	26.443706563706566	26.481544401544397	26.380772200772203	20.693976833976834
37	18.943861003861002	32.18378378378378	28.10957528957529	20.762779922779924
38	19.549575289575287	32.05119691119691	26.58285714285714	21.81637065637066
39	19.44857142857143	31.72679536679537	27.463629343629343	21.361003861003862
40	22.93096525096525	27.699150579150576	26.34849420849421	23.021389961389964
41	17.504169884169883	25.006486486486484	29.19366795366795	28.295675675675675
42	20.71073359073359	25.87158301158301	26.568957528957526	26.84872586872587
43	20.445250965250967	26.090115830115828	28.29706563706564	25.167567567567566
44	19.360077220077223	28.820077220077224	28.711814671814672	23.10803088803089
45	18.384555984555984	32.94007722007722	25.66702702702703	23.00833976833977
46	21.68200772200772	30.242471042471042	27.156138996138996	20.91938223938224
47	20.3619305019305	26.98895752895753	27.394285714285715	25.254826254826256
48	20.102779922779924	25.016293436293434	32.134903474903474	22.746023166023164
49	20.887876447876447	23.992741312741312	31.44084942084942	23.67853281853282
50	18.985945945945947	30.19366795366795	29.237760617760618	21.582625482625485
51	17.765405405405403	29.058223938223936	27.913976833976832	25.262393822393825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1882.0
1	2088.5
2	2295.0
3	7031.5
4	11768.0
5	8454.5
6	5141.0
7	5891.5
8	6642.0
9	7880.0
10	9118.0
11	9737.5
12	10357.0
13	10720.0
14	11083.0
15	10814.5
16	10546.0
17	10211.0
18	9876.0
19	9660.5
20	9445.0
21	9188.5
22	8932.0
23	9467.0
24	10002.0
25	11023.5
26	11811.0
27	11577.0
28	13806.0
29	16035.0
30	17023.5
31	18012.0
32	22077.5
33	26143.0
34	28017.0
35	29891.0
36	35526.0
37	41161.0
38	44724.0
39	48287.0
40	62733.5
41	77180.0
42	90200.0
43	103220.0
44	112250.0
45	121280.0
46	123557.0
47	125834.0
48	128255.0
49	130676.0
50	117738.5
51	104801.0
52	93639.5
53	82478.0
54	71530.5
55	60583.0
56	54841.0
57	49099.0
58	43663.0
59	38227.0
60	33914.5
61	29602.0
62	25545.5
63	21489.0
64	18188.5
65	14888.0
66	11982.5
67	9077.0
68	7627.0
69	6177.0
70	5038.5
71	3900.0
72	3174.5
73	2449.0
74	2118.5
75	1401.0
76	1014.0
77	749.0
78	484.0
79	409.0
80	334.0
81	215.0
82	96.0
83	77.5
84	59.0
85	39.0
86	19.0
87	11.0
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1295000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.51818832735917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25031545159668	16.67109274093201
2	8.011561511068024	3.2876545576063174
3	2.7442925075587805	1.6892373148635538
4	1.3455017004462044	1.104290291381489
5	0.8053158326890276	0.8261810959058767
6	0.5235319580639848	0.6445156386568767
7	0.3550650280506246	0.5099703779801247
8	0.28076247333893734	0.4608589842578779
9	0.22124978099596918	0.408568020647604
>10	2.049396093559209	10.105922776579584
>50	1.0763384341896693	16.26772840722504
>100	1.3271542140034946	45.133469799730186
>500	0.00532840808604787	0.7275082700134725
>1k	0.003806005775748479	1.5491178136815429
>5k	3.806005775748479E-4	0.6138839105384186
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7861	0.607027027027027	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4052	0.31289575289575294	No Hit
GAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	2800	0.21621621621621623	Illumina PCR Primer Index 8 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	2092	0.16154440154440153	TruSeq Adapter, Index 19 (95% over 22bp)
AAGAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1839	0.14200772200772202	No Hit
GTCCCAGGGATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGT	1733	0.1338223938223938	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1542	0.11907335907335907	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	1507	0.11637065637065637	RNA PCR Primer, Index 40 (96% over 26bp)
TAGAGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1484	0.11459459459459459	RNA PCR Primer, Index 40 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1411	0.10895752895752894	Illumina PCR Primer Index 8 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1377	0.10633204633204633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1322007722007722	0.0
2	0.0	0.0	0.0	0.4240926640926641	0.0
3	0.0	0.0	0.0	0.6149034749034749	0.0
4	0.0	0.0	0.0	1.3094208494208495	0.0
5	0.0	0.0	0.0	1.9341312741312742	0.0
6	0.0	0.0	0.0	2.9755984555984556	0.0
7	0.0	7.722007722007723E-5	0.0	3.3147490347490347	0.0
8	0.0	7.722007722007723E-5	0.0	4.265173745173745	0.0
9	0.0	7.722007722007723E-5	0.0	4.552586872586873	0.0
10	0.0	7.722007722007723E-5	0.0	5.09034749034749	0.0
11	0.0	7.722007722007723E-5	0.0	5.7877220077220075	0.0
12	0.0	7.722007722007723E-5	0.0	6.3566023166023164	0.0
13	0.0	7.722007722007723E-5	0.0	6.588494208494208	0.0
14	0.0	7.722007722007723E-5	0.0	6.6905791505791505	0.0
15	0.0	7.722007722007723E-5	0.0	6.824787644787645	0.0
16	0.0	7.722007722007723E-5	0.0	7.132277992277992	0.0
17	0.0	7.722007722007723E-5	0.0	7.465173745173745	0.0
18	0.0	7.722007722007723E-5	0.0	7.89027027027027	0.0
19	0.0	7.722007722007723E-5	0.0	8.102625482625482	0.0
20	0.0	7.722007722007723E-5	0.0	8.304787644787645	0.0
21	0.0	7.722007722007723E-5	0.0	8.552818532818533	0.0
22	0.0	7.722007722007723E-5	0.0	8.805096525096525	0.0
23	0.0	7.722007722007723E-5	0.0	9.081930501930502	0.0
24	0.0	7.722007722007723E-5	0.0	9.280463320463321	0.0
25	0.0	7.722007722007723E-5	0.0	9.453127413127413	0.0
26	0.0	7.722007722007723E-5	0.0	9.612586872586872	0.0
27	0.0	7.722007722007723E-5	0.0	9.761235521235522	0.0
28	0.0	7.722007722007723E-5	0.0	9.92918918918919	0.0
29	0.0	7.722007722007723E-5	0.0	10.114440154440155	0.0
30	0.0	7.722007722007723E-5	0.0	10.337297297297297	0.0
31	0.0	7.722007722007723E-5	0.0	10.550656370656371	0.0
32	0.0	7.722007722007723E-5	0.0	10.727413127413127	0.0
33	0.0	7.722007722007723E-5	0.0	10.913667953667954	0.0
34	0.0	7.722007722007723E-5	0.0	11.103706563706563	0.0
35	0.0	7.722007722007723E-5	0.0	11.32015444015444	0.0
36	0.0	7.722007722007723E-5	0.0	11.518996138996139	0.0
37	0.0	7.722007722007723E-5	0.0	11.723243243243243	0.0
38	0.0	7.722007722007723E-5	0.0	11.92023166023166	0.0
39	0.0	7.722007722007723E-5	0.0	12.135135135135135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	45	3.8562575E-10	45.000004	1
CGTTCGA	45	3.8562575E-10	45.000004	33
AGTCGAT	45	3.8562575E-10	45.000004	42
AACGTTG	20	7.033619E-4	45.0	1
TTTAGCG	70	0.0	45.0	1
AACCGGT	20	7.033619E-4	45.0	45
TCCGCGA	50	2.1827873E-11	45.0	29
GTCGACG	25	3.8910803E-5	45.0	1
TACTTAC	20	7.033619E-4	45.0	44
ACGTTCA	20	7.033619E-4	45.0	15
TAGGTTA	20	7.033619E-4	45.0	38
CAGTTGA	40	6.8157533E-9	45.0	20
CATTGCG	20	7.033619E-4	45.0	1
ATTACCG	20	7.033619E-4	45.0	18
CGCGATA	25	3.8910803E-5	45.0	39
TCGGCCA	20	7.033619E-4	45.0	43
ATCGTCC	25	3.8910803E-5	45.0	37
CTGCGCG	20	7.033619E-4	45.0	1
TAGCACG	20	7.033619E-4	45.0	1
GTTACGA	25	3.8910803E-5	45.0	16
>>END_MODULE