SRR1547450_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1547450_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2432625
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53598	2.2032989055033143	No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	4063	0.16702122193104157	Illumina PCR Primer Index 11 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3266	0.1342582601099635	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	3026	0.1243923744925749	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	3007	0.12361132521453162	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2805	0.11530753815322953	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	2759	0.11341657674323005	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	2658	0.109264683212579	Illumina PCR Primer Index 11 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2615	0.10749704537279688	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	2612	0.10737372180257951	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	2457	0.10100200400801602	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAA	40	6.8212103E-9	45.0	28
TACCTCG	20	7.034843E-4	45.0	29
CGTAAAT	35	1.2126839E-7	45.0	16
TAAATCG	20	7.034843E-4	45.0	35
GCGTACT	20	7.034843E-4	45.0	18
CTTACCG	20	7.034843E-4	45.0	1
CGGTCGA	30	2.1665055E-6	44.999996	40
CGTTTTT	15625	0.0	43.632	1
CGCATCG	320	0.0	43.59375	21
CGGTCTA	1070	0.0	42.897194	31
ACGGGTA	170	0.0	42.35294	5
CCGCATC	335	0.0	42.313435	20
TGTTACG	70	0.0	41.785713	1
CGACGGT	1105	0.0	41.742085	28
ACGGTCT	1100	0.0	41.727272	30
ATAACGC	365	0.0	41.301373	11
TCACGAC	1155	0.0	41.298702	25
TAGCACG	60	3.6379788E-12	41.249996	1
CGTTTCG	60	3.6379788E-12	41.249996	42
TGCGTAG	205	0.0	40.609756	1