FastQCFastQC Report
Thu 26 May 2016
SRR1547449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547449_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3011731
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135560.45010659982581447No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53070.17621095642339904No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC207.035108E-445.00000425
GTCGACG207.035108E-445.00000425
CGCTATA207.035108E-445.00000432
CGCTAGC302.1666783E-645.00000412
TCCCGTA207.035108E-445.00000429
ACGCGTT207.035108E-445.00000418
CGTTGCC502.1827873E-1145.027
GTTCGAC253.8923175E-545.032
CGACGGT4700.044.04255328
TAGTACG2000.041.6251
TCCGCTA556.184564E-1140.90909225
CTCACGA5150.040.19417624
CGTTTTT81550.040.0337221
TACGCTA451.9301297E-840.025
TGTCGCG451.9301297E-840.023
CGAAGTA451.9301297E-840.033
GACGGTC5200.039.80769329
CACGACG5250.039.4285726
CGAACTA403.4607183E-739.37500420
TATCGTG403.4607183E-739.3750041
Produced by FastQC (version 0.11.5)