##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547449_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3011731
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28186713886466	31.0	31.0	33.0	30.0	34.0
2	31.691716491280264	31.0	31.0	34.0	30.0	34.0
3	31.833142136532114	31.0	31.0	34.0	30.0	34.0
4	35.574642954500256	37.0	35.0	37.0	33.0	37.0
5	35.451072157506765	37.0	35.0	37.0	33.0	37.0
6	35.49159768916945	37.0	35.0	37.0	33.0	37.0
7	35.91820783463065	37.0	35.0	37.0	35.0	37.0
8	35.945891913985676	37.0	35.0	37.0	35.0	37.0
9	37.676550462176074	39.0	37.0	39.0	35.0	39.0
10	36.99327330362506	39.0	37.0	39.0	33.0	39.0
11	36.81212365911829	39.0	37.0	39.0	32.0	39.0
12	36.323645106418866	38.0	35.0	39.0	32.0	39.0
13	36.1365862356233	38.0	35.0	39.0	32.0	39.0
14	37.22177013816971	39.0	35.0	41.0	32.0	41.0
15	37.368468498680656	39.0	35.0	41.0	32.0	41.0
16	37.42628242694982	39.0	35.0	41.0	32.0	41.0
17	37.355792731821005	39.0	35.0	41.0	32.0	41.0
18	37.26799903444232	39.0	36.0	41.0	32.0	41.0
19	37.220267347913875	39.0	35.0	41.0	32.0	41.0
20	37.093465850701804	39.0	35.0	41.0	32.0	41.0
21	37.02146141205838	39.0	35.0	41.0	31.0	41.0
22	36.9424175001021	39.0	35.0	41.0	31.0	41.0
23	36.851669023561534	39.0	35.0	41.0	31.0	41.0
24	36.773709869838974	38.0	35.0	41.0	31.0	41.0
25	36.67375771607757	38.0	35.0	40.0	31.0	41.0
26	36.521113273396594	38.0	35.0	40.0	31.0	41.0
27	36.42281199748583	38.0	35.0	40.0	30.0	41.0
28	36.34264813158944	38.0	35.0	40.0	30.0	41.0
29	36.38641864097424	38.0	35.0	40.0	30.0	41.0
30	36.317779376710604	38.0	35.0	40.0	30.0	41.0
31	36.214691152695906	38.0	35.0	40.0	30.0	41.0
32	36.141480098986264	38.0	35.0	40.0	30.0	41.0
33	36.05327965877431	38.0	35.0	41.0	30.0	41.0
34	35.94166012834479	38.0	35.0	41.0	29.0	41.0
35	35.83177813689204	38.0	35.0	41.0	29.0	41.0
36	35.775075529653876	38.0	35.0	41.0	28.0	41.0
37	35.730223582385015	38.0	35.0	41.0	28.0	41.0
38	35.60828573335401	38.0	34.0	40.0	27.0	41.0
39	35.5127708948774	38.0	34.0	40.0	27.0	41.0
40	35.38023050531405	38.0	34.0	40.0	26.0	41.0
41	35.27607279667407	38.0	34.0	40.0	26.0	41.0
42	35.21460681581456	38.0	34.0	40.0	26.0	41.0
43	35.17440701045346	38.0	34.0	40.0	26.0	41.0
44	35.04449102526089	38.0	34.0	40.0	25.0	41.0
45	34.89808717976473	38.0	34.0	40.0	24.0	41.0
46	34.86204544828207	38.0	34.0	40.0	24.0	41.0
47	34.82132069563982	37.0	34.0	40.0	24.0	41.0
48	34.744217528059444	37.0	34.0	40.0	24.0	41.0
49	34.67730750189841	37.0	34.0	40.0	24.0	41.0
50	34.55970901783725	37.0	34.0	40.0	24.0	41.0
51	33.97385257846733	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	2.0
12	6.0
13	9.0
14	17.0
15	75.0
16	221.0
17	565.0
18	1301.0
19	2422.0
20	4355.0
21	6966.0
22	10777.0
23	15826.0
24	23090.0
25	32724.0
26	42257.0
27	49963.0
28	56894.0
29	66130.0
30	78165.0
31	94100.0
32	114127.0
33	144501.0
34	219166.0
35	308502.0
36	231738.0
37	293224.0
38	433856.0
39	780304.0
40	444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.460680585351085	23.80039917243605	28.766911785946352	12.972008456266511
2	31.048456850894052	25.64040413967914	29.815943057331477	13.495195952095324
3	29.55104556150599	26.044324675742953	30.619534081895093	13.78509568085596
4	25.33157841786003	29.698967138831456	29.980001534001545	14.989452909306975
5	23.572058726360357	32.70248903371517	29.109339446318412	14.61611279360607
6	21.82432627615149	41.69502521971584	26.25178012246114	10.228868381671537
7	89.39719383968887	3.2828961152241023	5.911484126570401	1.408425918516627
8	89.01528722186676	3.124714657451147	5.497702152018224	2.362295968663868
9	83.17572186891857	6.72902061970342	7.481478259512553	2.6137792518654552
10	32.08540204951903	43.1897470258798	13.100406377594812	11.624444547006355
11	34.05221117025391	24.544323513620572	26.01118758614232	15.392277729983189
12	31.717606917749297	23.85143294670075	26.291956353339657	18.1390037822103
13	24.090000069727342	30.662532609984094	28.389786471633755	16.85768084865481
14	19.37736139117338	33.37369771735922	29.22711224873669	18.021828642730707
15	17.524871909211015	27.589084151273802	37.793116317493165	17.09292762202202
16	20.68879989613946	25.892949934771732	35.623267815087075	17.794982354001736
17	20.692850722723907	25.702893120268712	28.535649432170402	25.06860672483698
18	21.131867354687387	26.06899487371216	32.512863864667864	20.286273906932593
19	25.149258018063364	26.83280810935638	28.671518140232312	19.34641573234794
20	26.975948383172334	26.751924391653837	27.495649511858794	18.776477713315035
21	23.47952058135338	28.28732048114523	30.22763321159825	18.005525725903144
22	23.682194724562052	24.796105628291503	29.188463378701485	22.33323626844496
23	19.652551970942955	29.960411471011188	28.744433018752343	21.642603539293514
24	19.57688120220564	26.726092071303846	34.712661921001576	18.984364805488937
25	20.449900738146933	28.214339195632014	31.193489724015855	20.142270342205197
26	19.690603178039474	32.366934497138026	27.989119878236135	19.953342446586365
27	18.543223149743454	31.713224056198907	29.592118286792545	20.151434507265094
28	17.3906633759788	29.560408947545447	33.75972156875896	19.28920610771679
29	18.818148101540277	26.76381124343442	32.72334082957608	21.694699825449216
30	20.500602477445696	29.858509939964755	29.784532549553727	19.85635503303582
31	23.63079571183482	28.087003786194714	27.50956177693161	20.772638725038856
32	24.215907728811107	28.31574267422954	28.1204065037681	19.347943093191258
33	23.85472009286354	27.375718482161922	27.49222955170963	21.27733187326491
34	20.017325584522656	29.161834174433242	29.20911595358284	21.611724287461264
35	19.810168969273818	27.200570037629525	30.72791029477732	22.261350698319337
36	23.94490078961235	28.58821056727842	27.773928016811595	19.692960626297634
37	19.76803373209626	31.814428313816872	28.18050483260291	20.237033121483957
38	20.019749439774003	31.463600168806575	27.27331889866658	21.24333149275284
39	20.185434887777163	29.711285636067764	28.890428793275362	21.212850682879715
40	22.66543725186612	27.498006960116957	28.414323855616587	21.422231932400337
41	18.98423199150256	26.696972604791064	29.87361753091495	24.44517787279143
42	21.38076076515466	27.83930570160483	28.103472720505252	22.676460812735264
43	20.77058010825004	27.00294282590311	29.17670934090727	23.049767724939578
44	20.44807454583427	28.166260532564163	29.58152637137912	21.804138550222447
45	18.89474856818222	31.082789266372064	27.18967929074675	22.832782874698967
46	21.05008714257681	29.781079385907972	28.630677839421914	20.538155632093304
47	20.680532225487603	28.039589193058745	29.143505844313456	22.136372737140203
48	20.30148110837256	27.51646810422312	31.06436132576249	21.117689461641824
49	20.83396558324764	27.138678719978643	30.797305602658405	21.23005009411531
50	19.928041382181874	29.82753107764273	29.043297691593306	21.201129848582095
51	19.03958222032446	29.8577462595431	28.57117717352579	22.531494346606653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2417.0
1	3756.0
2	5095.0
3	13787.5
4	22480.0
5	16052.0
6	9624.0
7	10551.0
8	11478.0
9	12066.0
10	12654.0
11	13559.5
12	14465.0
13	14529.5
14	14594.0
15	15405.5
16	16217.0
17	15412.5
18	14608.0
19	15619.0
20	16630.0
21	16916.5
22	17203.0
23	17626.0
24	18049.0
25	23011.0
26	34197.0
27	40421.0
28	47800.0
29	55179.0
30	60216.5
31	65254.0
32	72416.5
33	79579.0
34	95223.5
35	110868.0
36	118270.0
37	125672.0
38	135028.5
39	144385.0
40	167454.5
41	190524.0
42	213441.5
43	236359.0
44	245123.0
45	253887.0
46	257753.0
47	261619.0
48	262749.5
49	263880.0
50	247339.0
51	230798.0
52	206457.5
53	182117.0
54	164510.5
55	146904.0
56	129277.0
57	111650.0
58	101100.5
59	90551.0
60	77958.0
61	65365.0
62	57454.5
63	49544.0
64	43459.0
65	37374.0
66	31140.5
67	24907.0
68	19831.5
69	14756.0
70	11697.5
71	8639.0
72	7796.5
73	6954.0
74	5831.0
75	3842.0
76	2976.0
77	2308.0
78	1640.0
79	1331.0
80	1022.0
81	690.0
82	358.0
83	294.0
84	230.0
85	157.0
86	84.0
87	55.0
88	26.0
89	17.5
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3011731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.499074546008348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17095093893045	13.062474214197831
2	8.960509372836684	2.9568022422527793
3	3.082439540088776	1.525721992664652
4	1.578072026674111	1.0414691202826651
5	0.8555382949062209	0.7057795052311305
6	0.5766193416293205	0.5708211301327448
7	0.3959735934442263	0.45732384855409636
8	0.3006201288805183	0.3967963133144247
9	0.2371363848172428	0.352127780160355
>10	2.06304037490049	7.778808509012134
>50	0.9082392017466613	11.115887900775554
>100	1.8592443456811936	57.77079257797348
>500	0.008967088428449777	0.9642890191239548
>1k	0.0022417721071124443	0.6666432140718156
>5k	2.0379746428294952E-4	0.17844625930995286
>10k+	2.0379746428294952E-4	0.4558163729424762
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13556	0.45010659982581447	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5307	0.17621095642339904	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05740884561071357	0.0
2	0.0	0.0	0.0	0.1864376333742954	0.0
3	0.0	0.0	0.0	0.28508522175453255	0.0
4	0.0	0.0	0.0	0.6097490114488977	0.0
5	0.0	0.0	0.0	0.9738585550967201	0.0
6	0.0	0.0	0.0	1.577896565131481	0.0
7	0.0	0.0	0.0	1.7967740146779376	0.0
8	0.0	0.0	0.0	2.415023121254853	0.0
9	0.0	0.0	0.0	2.5970778930787644	0.0
10	0.0	0.0	0.0	2.975830178724461	0.0
11	0.0	0.0	0.0	3.448647970220448	0.0
12	0.0	0.0	0.0	3.8427402712924894	0.0
13	0.0	0.0	0.0	4.014302738192754	0.0
14	0.0	0.0	0.0	4.079813236972359	0.0
15	0.0	0.0	0.0	4.184171826766733	0.0
16	0.0	0.0	0.0	4.401887153932407	0.0
17	0.0	0.0	0.0	4.639358561571402	0.0
18	0.0	0.0	0.0	4.947254585485888	0.0
19	0.0	0.0	0.0	5.09540858728751	0.0
20	0.0	0.0	0.0	5.266340187752492	0.0
21	0.0	0.0	0.0	5.4785105309869975	0.0
22	0.0	0.0	0.0	5.7122963505040785	0.0
23	0.0	0.0	0.0	5.953254125285426	0.0
24	0.0	0.0	0.0	6.141584357965568	0.0
25	0.0	0.0	0.0	6.302156467493279	0.0
26	0.0	0.0	0.0	6.455888656722662	0.0
27	0.0	0.0	0.0	6.613406044563741	0.0
28	0.0	0.0	0.0	6.775339497451798	0.0
29	0.0	0.0	0.0	6.9621091657920315	0.0
30	0.0	0.0	0.0	7.176304922318759	0.0
31	0.0	0.0	0.0	7.371275854317666	0.0
32	0.0	0.0	0.0	7.558676389093183	0.0
33	0.0	0.0	0.0	7.74913164555533	0.0
34	0.0	0.0	0.0	7.949747171975186	0.0
35	0.0	0.0	0.0	8.170915662786618	0.0
36	0.0	0.0	0.0	8.38003128433449	0.0
37	0.0	0.0	0.0	8.58815080098455	0.0
38	0.0	0.0	0.0	8.79783088197452	0.0
39	0.0	0.0	0.0	9.069236263132398	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	20	7.035108E-4	45.000004	25
GTCGACG	20	7.035108E-4	45.000004	25
CGCTATA	20	7.035108E-4	45.000004	32
CGCTAGC	30	2.1666783E-6	45.000004	12
TCCCGTA	20	7.035108E-4	45.000004	29
ACGCGTT	20	7.035108E-4	45.000004	18
CGTTGCC	50	2.1827873E-11	45.0	27
GTTCGAC	25	3.8923175E-5	45.0	32
CGACGGT	470	0.0	44.042553	28
TAGTACG	200	0.0	41.625	1
TCCGCTA	55	6.184564E-11	40.909092	25
CTCACGA	515	0.0	40.194176	24
CGTTTTT	8155	0.0	40.033722	1
TACGCTA	45	1.9301297E-8	40.0	25
TGTCGCG	45	1.9301297E-8	40.0	23
CGAAGTA	45	1.9301297E-8	40.0	33
GACGGTC	520	0.0	39.807693	29
CACGACG	525	0.0	39.42857	26
CGAACTA	40	3.4607183E-7	39.375004	20
TATCGTG	40	3.4607183E-7	39.375004	1
>>END_MODULE