FastQCFastQC Report
Thu 26 May 2016
SRR1547448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547448_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4101465
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314690.7672624294002265No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG207.035408E-445.00000417
TACGTCG207.035408E-445.00000443
TCGATAG1350.041.6666641
CGTTTTT150300.041.197611
CGCATCG1000.040.521
CACGACG4200.040.1785726
GGGCGAT70800.038.485177
AGGGCGA39650.037.67976
GCGTAAG3000.037.51
ATCGATA301.1404334E-437.49999621
TACGGGA14100.037.021284
ATAGGGC18600.037.016134
CGACGGT4500.037.028
TAAGGGA53850.036.9777154
AGGGATC36700.036.9686626
AAGGGAT59850.036.503765
ATACGGG12150.036.481483
CGGTCTA4600.036.19565631
ACGGGAT15500.036.145165
AGGGTAC14850.036.0606086
Produced by FastQC (version 0.11.5)