FastQCFastQC Report
Thu 26 May 2016
SRR1547447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547447_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences849666
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156281.8393109763130453No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT9680.11392711959758305No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC207.032246E-445.00000444
TATAGCG453.8380676E-1045.0000041
CGTTTTT78600.043.1679421
TATACGG700.041.7857172
CGTTAGG1350.041.6666642
TAGGGCG2400.041.2499965
CGACGGT556.002665E-1140.90909228
CTCGTAC1050.040.71428729
TACGGGA3050.040.5737724
TGTAGCG501.0804797E-940.51
CGATACG451.9272193E-840.00000410
AGGCGTC1300.039.8076935
TAGGGAC8350.039.3413165
TCTCGTA1100.038.86363628
TAACGCC356.246366E-638.5714312
ACACGAC1650.038.18181626
CGACGTT659.094947E-1238.0769227
AGGGCGA7200.037.8125046
TAGGGTC2500.037.85
GTTTTTT91750.037.6675762
Produced by FastQC (version 0.11.5)