FastQCFastQC Report
Thu 26 May 2016
SRR1547446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547446_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2301858
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113110.49138565454515437No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27280.11851295779322618No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG207.034762E-445.00000418
TTGCGAT207.034762E-445.00000427
CATACGT406.8193913E-945.00000432
GTACGAC207.034762E-445.00000436
TTAGGCG253.8920298E-545.040
TAAGTCG351.2126475E-745.01
CGACGGT3300.044.31818428
CGGTCTA3350.043.65671531
CGTAAGG4300.042.9069752
AACGGGA7100.041.8309864
TCACGAC3650.041.30137325
GAGCGTA603.6379788E-1241.2500049
CGTATGG1100.040.9090922
AATACGG2100.040.7142872
TACGGGT1000.040.54
TGATTCG1000.040.515
AGGGCGC2950.040.423736
TACGGGA7350.040.4081654
TAATACG451.9297659E-840.01
TATTGCG2100.039.6428571