##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547446_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2301858
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252129366798474	31.0	31.0	33.0	30.0	34.0
2	31.640568184484014	31.0	31.0	34.0	30.0	34.0
3	31.790786399508573	31.0	31.0	34.0	30.0	34.0
4	35.524818646502084	37.0	35.0	37.0	33.0	37.0
5	35.39580851642456	37.0	35.0	37.0	33.0	37.0
6	35.44082736641444	37.0	35.0	37.0	33.0	37.0
7	35.87858981744313	37.0	35.0	37.0	35.0	37.0
8	35.909522655176815	37.0	35.0	37.0	35.0	37.0
9	37.633037311597846	39.0	37.0	39.0	35.0	39.0
10	36.945966692993224	39.0	37.0	39.0	32.0	39.0
11	36.65754664275555	39.0	35.0	39.0	32.0	39.0
12	36.052606633423956	38.0	35.0	39.0	31.0	39.0
13	35.797809856211806	38.0	35.0	39.0	31.0	39.0
14	36.867341947244356	39.0	35.0	41.0	31.0	41.0
15	37.06815972140766	39.0	35.0	41.0	32.0	41.0
16	37.16611363515908	39.0	35.0	41.0	32.0	41.0
17	37.08413203594662	39.0	35.0	41.0	32.0	41.0
18	37.01951944907114	38.0	35.0	41.0	32.0	41.0
19	36.960301634592575	38.0	35.0	41.0	31.0	41.0
20	36.83193359451365	38.0	35.0	41.0	31.0	41.0
21	36.74267743709647	38.0	35.0	41.0	31.0	41.0
22	36.62982946819482	38.0	35.0	40.0	31.0	41.0
23	36.579542265422106	38.0	35.0	40.0	31.0	41.0
24	36.472851062055085	38.0	35.0	40.0	31.0	41.0
25	36.37682254943615	38.0	35.0	40.0	31.0	41.0
26	36.18640202827455	38.0	35.0	40.0	30.0	41.0
27	36.091732852330594	38.0	35.0	40.0	30.0	41.0
28	35.99044206897211	38.0	35.0	40.0	30.0	41.0
29	36.08931784671339	38.0	35.0	40.0	30.0	41.0
30	36.02727883301229	38.0	35.0	40.0	30.0	41.0
31	35.956223190135965	38.0	35.0	40.0	30.0	41.0
32	35.84464376169164	38.0	34.0	40.0	30.0	41.0
33	35.7866753726772	38.0	34.0	40.0	29.0	41.0
34	35.6907771895573	38.0	34.0	40.0	29.0	41.0
35	35.615633110296116	38.0	34.0	40.0	29.0	41.0
36	35.54625393920911	38.0	34.0	40.0	28.0	41.0
37	35.49910724293158	38.0	34.0	40.0	27.0	41.0
38	35.4159748342426	38.0	34.0	40.0	27.0	41.0
39	35.362417664338984	38.0	34.0	40.0	27.0	41.0
40	35.24583097654156	37.0	34.0	40.0	27.0	41.0
41	35.16841699183877	37.0	34.0	40.0	27.0	41.0
42	35.12665073171325	37.0	34.0	40.0	27.0	41.0
43	35.09265297859381	37.0	34.0	40.0	27.0	41.0
44	34.95616236970309	37.0	34.0	40.0	26.0	41.0
45	34.8207508890644	37.0	34.0	40.0	26.0	41.0
46	34.77673905167043	37.0	34.0	40.0	26.0	41.0
47	34.72927435141525	36.0	33.0	40.0	26.0	41.0
48	34.647408311025266	36.0	33.0	40.0	26.0	41.0
49	34.56880398356458	36.0	33.0	40.0	26.0	41.0
50	34.44427154064238	36.0	33.0	40.0	25.0	41.0
51	33.8978603371711	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	3.0
13	6.0
14	25.0
15	70.0
16	192.0
17	404.0
18	1014.0
19	1807.0
20	3222.0
21	5320.0
22	8139.0
23	12008.0
24	16797.0
25	24073.0
26	31969.0
27	38823.0
28	45092.0
29	52734.0
30	62305.0
31	76619.0
32	94152.0
33	119290.0
34	191335.0
35	273758.0
36	178323.0
37	217131.0
38	312221.0
39	534693.0
40	327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.821305223866986	24.003001053931218	28.482295606418816	12.693398115782989
2	30.542718099900167	26.092573912031064	29.7017452857648	13.662962702303966
3	30.14247620834995	26.04357002039222	30.263856415122042	13.550097356135781
4	26.351408297123452	28.285193960704785	30.66466306783477	14.698734674336992
5	23.763238218864934	31.936287989962892	29.024987640419177	15.275486150753
6	21.78627004793519	41.97639472113397	25.7008903242511	10.536444906679735
7	88.28011979887552	4.198043493560419	5.7313266065934565	1.7905101009706073
8	88.49855203926568	3.62242153946942	5.459763373761544	2.4192630475033647
9	83.52187667527711	6.258683202873505	7.401108148287166	2.8183319735622265
10	42.50127505693227	33.49724439995864	12.776331120338439	11.225149422770649
11	37.1520310983562	23.131270478022536	23.859377945989717	15.857320477631548
12	34.73446233434034	21.411659624529403	26.93476313482413	16.919114906306124
13	23.141566508446655	31.912568021137705	27.000970520336182	17.94489495007946
14	17.97013542972677	36.00361099598672	28.313823007327127	17.712430566959387
15	17.049922280175405	27.112967003177435	39.516686085762025	16.320424630885135
16	19.94988396330269	23.578691648225043	38.717288381820254	17.75413600665202
17	19.16343232293217	23.88162084715912	28.811203818828098	28.143743011080613
18	22.338041703701965	26.44850377390786	30.236356890824716	20.97709763156546
19	26.932677862839498	26.99241221656592	26.259960431964092	19.81494948863049
20	30.30968895561759	24.653996901633377	26.23745687179661	18.79885727095242
21	24.691531797356745	27.935302698950153	27.66969117990771	19.703474323785393
22	23.550236374268092	25.75354344186305	26.45089314805692	24.24532703581194
23	22.325703844459564	29.17273784916359	25.91549956600277	22.586058740374078
24	21.562016423254608	25.708840423692514	33.76294280533378	18.966200347719102
25	21.048083765375623	26.500635573523653	30.823621613496577	21.627659047604155
26	19.57701126655076	32.64649687339532	27.157887237179708	20.61860462287422
27	21.005639791855103	32.316068150163915	27.319191713824225	19.35910034415676
28	18.660881774635968	29.797537467558815	32.75658185691733	18.784998900887892
29	20.29512680625825	27.00653124562853	31.668808414767547	21.02953353334567
30	21.40957435254477	29.080160461679217	29.831683796307157	19.678581389468857
31	27.262889370239172	27.87739295821028	24.88876377256981	19.97095389898074
32	28.186664859430948	27.367500514801524	25.93830722833468	18.507527397432856
33	25.922363586285513	29.139199724744095	25.076829239683768	19.86160744928662
34	21.16190486120343	28.627786770513215	28.465178998878297	21.745129369405063
35	21.708854325505744	27.165055359626876	29.056179833855957	22.069910481011426
36	26.051520119833633	29.628196005140197	25.646542923151642	18.673740951874528
37	21.878586776421482	31.214392894783256	28.094478460443696	18.812541868351566
38	21.031401589498568	32.16219245496465	25.51955854792085	21.286847407615937
39	21.853563512605902	29.91826602683571	27.626334899893912	20.601835560664473
40	23.471647686347293	26.873508270275575	28.272899544628732	21.381944498748403
41	19.198968833003597	26.764031491082424	29.088501549617746	24.948498126296233
42	22.00861217329653	27.595229592789828	26.298494520513426	24.09766371340022
43	22.756051850287896	26.731058127825435	27.071739438314612	23.441150583572053
44	20.983310004353008	29.51819790795088	27.589929526495556	21.90856256120056
45	20.50582616303873	32.18565176479174	25.03151801718438	22.27700405498515
46	21.936279301329623	31.380823665056663	26.186063606008712	20.496833427605004
47	21.894617304803337	27.739808450390946	28.047168852292366	22.318405392513352
48	22.119001258982962	25.72843329171478	30.433458536538744	21.719106912763518
49	21.87241784680028	26.578398841283867	29.454901214584044	22.094282097331806
50	19.823638121899787	29.572979740713805	28.534557735533646	22.068824401852762
51	18.87944434452516	30.48650264264781	27.12035234145634	23.513700671370692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3029.0
1	3568.0
2	4107.0
3	11041.5
4	17976.0
5	12695.0
6	7414.0
7	7544.0
8	7674.0
9	8536.0
10	9398.0
11	9841.5
12	10285.0
13	10050.5
14	9816.0
15	9445.0
16	9074.0
17	8577.5
18	8081.0
19	8110.5
20	8140.0
21	9518.5
22	10897.0
23	12167.0
24	13437.0
25	14173.5
26	19694.5
27	24479.0
28	28820.0
29	33161.0
30	36164.5
31	39168.0
32	47347.5
33	55527.0
34	57844.5
35	60162.0
36	68276.5
37	76391.0
38	88933.5
39	101476.0
40	124188.5
41	146901.0
42	164828.0
43	182755.0
44	197143.0
45	211531.0
46	211898.5
47	212266.0
48	199974.5
49	187683.0
50	170439.5
51	153196.0
52	141679.5
53	130163.0
54	125102.0
55	120041.0
56	110277.0
57	100513.0
58	97928.5
59	95344.0
60	86987.5
61	78631.0
62	67355.0
63	56079.0
64	46017.0
65	35955.0
66	29634.5
67	23314.0
68	18916.0
69	14518.0
70	12724.5
71	10931.0
72	9267.0
73	7603.0
74	6458.0
75	3767.0
76	2221.0
77	1789.0
78	1357.0
79	951.0
80	545.0
81	376.5
82	208.0
83	139.0
84	70.0
85	58.0
86	46.0
87	33.5
88	21.0
89	21.5
90	22.0
91	19.5
92	17.0
93	13.5
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2301858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.519217967136782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.98487719905607	13.662340617906585
2	8.745040740711412	3.06412549736029
3	3.1231959940050795	1.6414785411919024
4	1.5612891635920598	1.0941026066679187
5	0.8757693675526366	0.7671397219548084
6	0.5628974411089518	0.591691377835876
7	0.3999780139934057	0.49051114064490714
8	0.31515791368164336	0.44170561470854314
9	0.2459590379288393	0.38781089968163385
>10	2.9970939981465534	13.22094063549413
>50	1.6140801064112593	20.656236927367285
>100	1.563370433311409	41.78916785660663
>500	0.008028864356029418	0.9224620894916882
>1k	0.0030108241335110317	0.7730993629076437
>5k	0.0	0.0
>10k+	2.509020111259193E-4	0.4971871101801432
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11311	0.49138565454515437	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2728	0.11851295779322618	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07011727048323571	0.0
2	0.0	0.0	0.0	0.2518400353106056	0.0
3	0.0	0.0	0.0	0.3758268320634896	0.0
4	0.0	0.0	0.0	0.6416990101040116	0.0
5	0.0	0.0	0.0	1.0875127831516975	0.0
6	0.0	0.0	0.0	1.613001323278847	0.0
7	0.0	0.0	0.0	1.8572822476451631	0.0
8	0.0	0.0	0.0	2.539209629786025	0.0
9	0.0	0.0	0.0	2.7557738140232804	0.0
10	0.0	0.0	0.0	3.1623149646937385	0.0
11	0.0	0.0	0.0	3.6679065346341955	0.0
12	0.0	0.0	0.0	4.061588508066093	0.0
13	0.0	0.0	0.0	4.242746511730958	0.0
14	0.0	0.0	0.0	4.317555644179615	0.0
15	0.0	0.0	0.0	4.42729308237085	0.0
16	0.0	0.0	0.0	4.671009245574662	0.0
17	0.0	0.0	0.0	4.953302940494157	0.0
18	0.0	0.0	0.0	5.3171394586460154	0.0
19	0.0	0.0	0.0	5.498297462310881	0.0
20	0.0	0.0	0.0	5.68523340709983	0.0
21	0.0	0.0	0.0	5.920912584529541	0.0
22	0.0	0.0	0.0	6.181832241606563	0.0
23	0.0	0.0	0.0	6.445010943333602	0.0
24	0.0	0.0	0.0	6.660228389414117	0.0
25	0.0	0.0	0.0	6.830829703656785	0.0
26	0.0	0.0	0.0	7.00060559773887	0.0
27	0.0	0.0	0.0	7.177636500600819	0.0
28	0.0	0.0	0.0	7.349280450835803	0.0
29	0.0	0.0	0.0	7.537954122278611	0.0
30	0.0	0.0	0.0	7.760643792970722	0.0
31	0.0	0.0	0.0	7.976252227548354	0.0
32	0.0	0.0	0.0	8.178393280558575	0.0
33	0.0	0.0	0.0	8.382315503389002	0.0
34	0.0	0.0	0.0	8.588974645699256	0.0
35	0.0	0.0	0.0	8.828129276436687	0.0
36	0.0	0.0	0.0	9.06267893154139	0.0
37	0.0	0.0	0.0	9.290798998026812	0.0
38	0.0	0.0	0.0	9.523350267479575	0.0
39	0.0	0.0	0.0	9.749428505146712	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTCG	20	7.034762E-4	45.000004	18
TTGCGAT	20	7.034762E-4	45.000004	27
CATACGT	40	6.8193913E-9	45.000004	32
GTACGAC	20	7.034762E-4	45.000004	36
TTAGGCG	25	3.8920298E-5	45.0	40
TAAGTCG	35	1.2126475E-7	45.0	1
CGACGGT	330	0.0	44.318184	28
CGGTCTA	335	0.0	43.656715	31
CGTAAGG	430	0.0	42.906975	2
AACGGGA	710	0.0	41.830986	4
TCACGAC	365	0.0	41.301373	25
GAGCGTA	60	3.6379788E-12	41.250004	9
CGTATGG	110	0.0	40.909092	2
AATACGG	210	0.0	40.714287	2
TACGGGT	100	0.0	40.5	4
TGATTCG	100	0.0	40.5	15
AGGGCGC	295	0.0	40.42373	6
TACGGGA	735	0.0	40.408165	4
TAATACG	45	1.9297659E-8	40.0	1
TATTGCG	210	0.0	39.642857	1
>>END_MODULE