FastQCFastQC Report
Thu 26 May 2016
SRR1547445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547445_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences283505
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27290.9625932523235922No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6150.21692739105130418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5690.2007019276555969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG302.159697E-645.0000043
CGGGTGA302.159697E-645.0000046
CAGCGAT551.8189894E-1245.00000421
GCGCGAC302.159697E-645.0000049
CGCGACC302.159697E-645.00000410
GTGGCGA302.159697E-645.00000428
CTACGAA302.159697E-645.00000411
CGAATAT302.159697E-645.00000414
GCGATGA551.8189894E-1245.0000049
GGCGCGA302.159697E-645.0000048
ACCCGCT302.159697E-645.00000434
GCTACGA302.159697E-645.00000410
CGTTTGT551.8189894E-1245.00000439
CCCGCTG302.159697E-645.00000435
CGGGTTG207.024288E-445.06
GTGCAAG453.8198777E-1045.01
CGGGTAT406.7830115E-945.06
CTAGAAG207.024288E-445.030
CGGGTAC207.024288E-445.06
AAGTACG207.024288E-445.01