##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547445_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	283505
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233681945644697	31.0	31.0	33.0	30.0	34.0
2	31.621449357154194	31.0	31.0	34.0	30.0	34.0
3	31.721444066242217	31.0	31.0	34.0	30.0	34.0
4	35.48917655773267	37.0	35.0	37.0	33.0	37.0
5	35.35729528579743	37.0	35.0	37.0	33.0	37.0
6	35.40794342251459	37.0	35.0	37.0	33.0	37.0
7	35.882407717676934	37.0	35.0	37.0	35.0	37.0
8	35.9367524382286	37.0	35.0	37.0	35.0	37.0
9	37.71108446059152	39.0	37.0	39.0	35.0	39.0
10	36.964617907973405	39.0	37.0	39.0	32.0	39.0
11	36.54771520784466	39.0	35.0	39.0	32.0	39.0
12	35.68126488068994	37.0	35.0	39.0	31.0	39.0
13	35.23155499903	37.0	35.0	39.0	30.0	39.0
14	36.24090933140509	38.0	35.0	40.0	30.0	41.0
15	36.588349411826954	38.0	35.0	41.0	31.0	41.0
16	36.78363697289289	38.0	35.0	41.0	32.0	41.0
17	36.74957055431121	38.0	35.0	40.0	32.0	41.0
18	36.66385072573676	38.0	35.0	40.0	31.0	41.0
19	36.54633604345602	37.0	35.0	40.0	31.0	41.0
20	36.376554205393205	37.0	35.0	40.0	31.0	41.0
21	36.24808380804571	37.0	35.0	40.0	31.0	41.0
22	36.13704520202466	37.0	35.0	40.0	31.0	41.0
23	36.14135200437382	37.0	35.0	40.0	31.0	41.0
24	36.052355337648365	37.0	35.0	40.0	31.0	41.0
25	35.95016666372727	37.0	35.0	40.0	31.0	41.0
26	35.77888220666302	36.0	34.0	40.0	30.0	41.0
27	35.68967037618384	36.0	34.0	40.0	30.0	41.0
28	35.66892294668524	36.0	34.0	40.0	30.0	41.0
29	35.69939154512266	36.0	34.0	40.0	30.0	41.0
30	35.66004126911342	36.0	34.0	40.0	30.0	41.0
31	35.51454824429904	36.0	34.0	40.0	30.0	41.0
32	35.304791802613714	36.0	34.0	40.0	29.0	41.0
33	35.19798592617414	36.0	34.0	40.0	29.0	41.0
34	35.1284986155447	36.0	34.0	40.0	28.0	41.0
35	35.01755877321388	36.0	34.0	40.0	27.0	41.0
36	34.88950106700058	36.0	34.0	40.0	26.0	41.0
37	34.8088181866281	36.0	34.0	40.0	26.0	41.0
38	34.755813830443905	36.0	34.0	40.0	27.0	41.0
39	34.71715842754096	36.0	34.0	40.0	26.0	41.0
40	34.60718153118992	36.0	34.0	40.0	25.0	41.0
41	34.576391950759245	36.0	34.0	40.0	25.0	41.0
42	34.52815999717818	36.0	34.0	40.0	25.0	41.0
43	34.46587185411192	35.0	33.0	40.0	25.0	41.0
44	34.35591259413414	35.0	33.0	40.0	24.0	41.0
45	34.20901571400857	35.0	33.0	40.0	24.0	41.0
46	34.2015625826705	35.0	33.0	40.0	24.0	41.0
47	34.18070228038306	35.0	33.0	40.0	24.0	41.0
48	34.14789157157722	35.0	33.0	40.0	24.0	41.0
49	34.116114354244196	35.0	33.0	40.0	24.0	41.0
50	33.95646637625439	35.0	33.0	40.0	24.0	41.0
51	33.36912223770304	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	19.0
17	50.0
18	117.0
19	255.0
20	462.0
21	780.0
22	1158.0
23	1680.0
24	2604.0
25	3612.0
26	4659.0
27	5277.0
28	5782.0
29	6647.0
30	8039.0
31	9789.0
32	12238.0
33	16089.0
34	27376.0
35	44128.0
36	21532.0
37	23675.0
38	32126.0
39	55375.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.72677730551489	25.12654097811326	29.581841590095415	13.564840126276431
2	29.488016084372408	26.391774395513306	31.364173471367344	12.756036048746935
3	27.4876986296538	26.151566991763815	33.28618542882841	13.074548949753972
4	23.594292869614293	28.58009558914305	32.895363397470945	14.930248143771715
5	22.73751785682792	32.00296291070705	31.748999135817712	13.510520096647324
6	20.797869526110652	40.99998236362674	27.58081868044655	10.621329429816052
7	87.43725860214106	4.257067776582424	6.8291564522671555	1.476517169009365
8	88.70178656461086	3.050739845858098	6.208356113648789	2.0391174758822594
9	85.28879561207033	4.923369958201795	7.59704414384226	2.1907902858856105
10	50.30493289359976	28.89331757817322	12.037882929754325	8.76386659847269
11	40.62397488580448	22.403484947355427	24.131849526463377	12.840690640376712
12	37.996860725560396	23.013350734554948	24.783689882012663	14.206098657871996
13	20.532265744872223	41.48498262817234	25.083155499902997	12.899596127052432
14	14.651946173788822	43.75972205075748	27.05137475529532	14.536957020158376
15	12.935927055960212	26.752261864870107	46.408352586374136	13.903458492795542
16	14.61808433713691	23.25038359111832	44.802737165129365	17.328794906615403
17	15.501666637272713	23.60628560342851	28.21643357260013	32.675614186698645
18	19.561207033385656	26.316290717976752	34.82054990211813	19.301952346519464
19	26.255621593975416	27.95118251882683	26.000599636690712	19.792596250507046
20	28.51977919260683	27.704626020705103	25.314897444489514	18.46069734219855
21	20.284298336890004	31.74053367665473	28.754695684379467	19.2204723020758
22	20.821854993739088	27.233382127299343	25.51383573481949	26.430927144142082
23	17.275885786846793	33.94754942593605	25.822472266803054	22.9540925204141
24	17.850478827533905	27.082767499691364	38.87127211160297	16.19548156117176
25	15.248055589848503	30.076365496199365	34.92460450432973	19.750974409622405
26	15.434295691434013	38.37569002310365	26.665490908449584	19.524523377012752
27	15.994779633516162	38.10444260242324	27.968113437152788	17.932664326907815
28	13.438563693762015	33.22022539285022	37.25648577626497	16.084725137122803
29	13.721451120791519	28.00973527803742	36.50341263822507	21.76540096294598
30	16.687889102484966	34.442426059505124	31.072115130244615	17.797569707765295
31	25.94275233241036	30.40263840143913	24.551595209961025	19.103014056189487
32	27.494400451491156	31.246009770550785	25.089504594275237	16.17008518368283
33	24.829897179943917	31.669988183629915	24.373115112608243	19.126999523817922
34	17.06671840002822	31.5920354138375	26.991411086224225	24.349835099910056
35	17.225093031868926	30.764536780656428	29.9948854517557	22.015484735718946
36	27.692280559425758	28.448880972116893	25.764977689987834	18.093860778469516
37	17.477645896897762	36.259677959824344	28.44076824041904	17.821907902858854
38	18.325955450521157	36.35032891836123	24.586162501543182	20.737553129574433
39	17.74007513095007	36.25438704784748	27.029152924992506	18.976384896209943
40	22.838397911853406	30.123630976525988	25.401668400910037	21.63630271071057
41	16.54256538685385	28.553288301793618	27.163189361739654	27.740956949612883
42	20.241265586144866	29.29401597855417	24.43942787605157	26.0252905592494
43	19.805647166716636	29.37020511102097	26.195657924904324	24.62848979735807
44	18.391562759034233	33.522865557926664	27.54307684167828	20.542494841360824
45	16.491772631875982	39.529108834059365	22.597837780638788	21.381280753425866
46	20.717800391527486	35.8222959030705	24.355126011886917	19.104777693515103
47	19.682192553923212	30.956773249149045	25.946279607061605	23.414754589866142
48	20.62538579566498	28.250648136717167	30.149732808945167	20.974233258672687
49	19.28537415565863	27.88980793989524	31.006860549196663	21.81795735524947
50	18.60108287331793	35.77820496992998	25.952628701433838	19.668083455318246
51	16.725278213788116	35.834641364349835	24.131849526463377	23.308230895398673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	659.0
1	576.5
2	494.0
3	1930.0
4	3366.0
5	2171.0
6	976.0
7	1164.5
8	1353.0
9	1712.0
10	2071.0
11	2313.0
12	2555.0
13	2664.0
14	2773.0
15	2530.0
16	2287.0
17	2089.0
18	1891.0
19	1745.0
20	1599.0
21	1569.5
22	1540.0
23	1594.5
24	1649.0
25	1741.0
26	2226.0
27	2619.0
28	2984.0
29	3349.0
30	3974.0
31	4599.0
32	5087.5
33	5576.0
34	6713.5
35	7851.0
36	8617.5
37	9384.0
38	11100.5
39	12817.0
40	16874.0
41	20931.0
42	26120.0
43	31309.0
44	32786.0
45	34263.0
46	32422.5
47	30582.0
48	28210.0
49	25838.0
50	22895.5
51	19953.0
52	17724.5
53	15496.0
54	13332.5
55	11169.0
56	9563.0
57	7957.0
58	6846.0
59	5735.0
60	4788.0
61	3841.0
62	3141.0
63	2441.0
64	1898.5
65	1356.0
66	956.5
67	557.0
68	446.5
69	336.0
70	276.0
71	216.0
72	176.5
73	137.0
74	103.5
75	48.5
76	27.0
77	25.5
78	24.0
79	19.5
80	15.0
81	10.5
82	6.0
83	5.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	283505.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.939807058076575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84648662540424	24.983686354738012
2	7.946587282890369	5.2351810373714756
3	3.3666716638468293	3.3269254510502457
4	1.8921465744329984	2.49307772349694
5	1.3749384276015677	2.2645103260965413
6	0.9787334289936392	1.9343574187404102
7	0.8106140106654102	1.869102837692457
8	0.6103698627203221	1.608437240965768
9	0.5771743087829011	1.7110809333168726
>10	6.252543207761335	45.50254845593552
>50	0.308397404321847	6.22740339676549
>100	0.032124729616859055	1.4634662527997742
>500	0.0021416486411239373	0.4176293187069012
>1k	0.0010708243205619686	0.9625932523235922
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2729	0.9625932523235922	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	615	0.21692739105130418	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	569	0.2007019276555969	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09382550572300312	0.0
2	0.0	0.0	0.0	0.3111056242394314	0.0
3	0.0	0.0	0.0	0.46983298354526376	0.0
4	0.0	0.0	0.0	0.7551895028306379	0.0
5	0.0	0.0	0.0	1.285691610377242	0.0
6	0.0	0.0	0.0	1.9156628630888344	0.0
7	0.0	0.0	0.0	2.258161231724308	0.0
8	0.0	0.0	0.0	3.0817798627890163	0.0
9	0.0	0.0	0.0	3.3777182060281126	0.0
10	0.0	0.0	0.0	3.880707571295039	0.0
11	0.0	0.0	0.0	4.538897021216557	0.0
12	0.0	0.0	0.0	5.061286397065308	0.0
13	0.0	0.0	0.0	5.288090157140085	0.0
14	0.0	0.0	0.0	5.390028394560942	0.0
15	0.0	0.0	0.0	5.524770286238338	0.0
16	0.0	0.0	0.0	5.802014073825858	0.0
17	0.0	0.0	0.0	6.157210631205799	0.0
18	0.0	0.0	0.0	6.590359958378159	0.0
19	0.0	0.0	0.0	6.818927355778558	0.0
20	0.0	0.0	0.0	7.054902029946562	0.0
21	0.0	0.0	0.0	7.371298566162854	0.0
22	0.0	0.0	0.0	7.71309147986808	0.0
23	0.0	0.0	0.0	8.077458951341246	0.0
24	0.0	0.0	0.0	8.362462743161496	0.0
25	0.0	0.0	0.0	8.60090650958537	0.0
26	0.0	0.0	0.0	8.82453572247403	0.0
27	0.0	0.0	0.0	9.027706742385496	0.0
28	0.0	0.0	0.0	9.251335955274158	0.0
29	0.0	0.0	0.0	9.506710640023986	0.0
30	0.0	0.0	0.0	9.829103543147387	0.0
31	0.0	0.0	0.0	10.128569161037724	0.0
32	0.0	0.0	0.0	10.372656566903581	0.0
33	0.0	0.0	0.0	10.640376712932753	0.0
34	0.0	0.0	0.0	10.897162307543077	0.0
35	0.0	0.0	0.0	11.23436976420169	0.0
36	0.0	0.0	0.0	11.52113719334756	0.0
37	0.0	0.0	0.0	11.808962804888802	0.0
38	0.0	0.0	0.0	12.094319324174176	0.0
39	0.0	0.0	0.0	12.375090386412937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	30	2.159697E-6	45.000004	3
CGGGTGA	30	2.159697E-6	45.000004	6
CAGCGAT	55	1.8189894E-12	45.000004	21
GCGCGAC	30	2.159697E-6	45.000004	9
CGCGACC	30	2.159697E-6	45.000004	10
GTGGCGA	30	2.159697E-6	45.000004	28
CTACGAA	30	2.159697E-6	45.000004	11
CGAATAT	30	2.159697E-6	45.000004	14
GCGATGA	55	1.8189894E-12	45.000004	9
GGCGCGA	30	2.159697E-6	45.000004	8
ACCCGCT	30	2.159697E-6	45.000004	34
GCTACGA	30	2.159697E-6	45.000004	10
CGTTTGT	55	1.8189894E-12	45.000004	39
CCCGCTG	30	2.159697E-6	45.000004	35
CGGGTTG	20	7.024288E-4	45.0	6
GTGCAAG	45	3.8198777E-10	45.0	1
CGGGTAT	40	6.7830115E-9	45.0	6
CTAGAAG	20	7.024288E-4	45.0	30
CGGGTAC	20	7.024288E-4	45.0	6
AAGTACG	20	7.024288E-4	45.0	1
>>END_MODULE