FastQCFastQC Report
Thu 26 May 2016
SRR1547444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547444_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4648805
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525861.131172419578795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT260600.042.8760571
TCGATAG1750.039.8571431
CGGTCTA6600.038.52272831
CACGACG6750.038.026
GGCGATA18500.037.70278
GCGATAT4500.037.5000049
GGGCGAT74100.037.2874537
TATGGGC21250.037.1647034
TACGGGA16050.037.1495324
TAATCCG850.037.0588231
TCTCACG7000.036.6428623
ATAGGGC29250.036.538464
AGGGCGA38700.036.395356
GTAGGGC26300.036.3593144
TAGGGAC45900.036.323535
TAGGGCA36600.036.2704935
TTAGGGA55750.036.2017944
ATAGGGA54450.036.157034
GTTTTTT320950.036.1317982
TAGGGAT55900.036.06445
Produced by FastQC (version 0.11.5)