##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547444_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4648805
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219524372392474	31.0	31.0	33.0	30.0	34.0
2	31.62600087549381	31.0	31.0	34.0	30.0	34.0
3	31.72545374564001	31.0	31.0	34.0	30.0	34.0
4	35.52602055797135	37.0	35.0	37.0	33.0	37.0
5	35.41154361174539	37.0	35.0	37.0	33.0	37.0
6	35.46001370244611	37.0	35.0	37.0	33.0	37.0
7	35.862927569558195	37.0	35.0	37.0	35.0	37.0
8	35.89693200725778	37.0	35.0	37.0	35.0	37.0
9	37.652425300695555	39.0	37.0	39.0	35.0	39.0
10	36.92230971185068	39.0	37.0	39.0	32.0	39.0
11	36.676741872373654	39.0	35.0	39.0	32.0	39.0
12	36.340828664570786	38.0	35.0	39.0	32.0	39.0
13	36.24633255212899	39.0	35.0	39.0	32.0	39.0
14	37.3244037983955	39.0	36.0	41.0	32.0	41.0
15	37.43404767461745	39.0	36.0	41.0	32.0	41.0
16	37.46835477074216	39.0	36.0	41.0	32.0	41.0
17	37.38863041147133	39.0	35.0	41.0	32.0	41.0
18	37.247131682228016	39.0	36.0	41.0	32.0	41.0
19	37.136859042270004	39.0	35.0	41.0	32.0	41.0
20	36.97769620364804	39.0	35.0	41.0	31.0	41.0
21	36.920416322044055	39.0	35.0	41.0	31.0	41.0
22	36.84520538073763	39.0	35.0	41.0	31.0	41.0
23	36.755200099810594	38.0	35.0	40.0	31.0	41.0
24	36.648430080418514	38.0	35.0	40.0	31.0	41.0
25	36.52531327943418	38.0	35.0	40.0	31.0	41.0
26	36.3772634042512	38.0	35.0	40.0	30.0	41.0
27	36.274232410264574	38.0	35.0	40.0	30.0	41.0
28	36.16052232778101	38.0	35.0	40.0	30.0	41.0
29	36.16732515130232	38.0	35.0	40.0	30.0	41.0
30	36.082217903310635	38.0	35.0	40.0	30.0	41.0
31	35.95353042340989	38.0	35.0	40.0	30.0	41.0
32	35.84764321153501	38.0	35.0	40.0	29.0	41.0
33	35.689738115494194	38.0	34.0	40.0	27.0	41.0
34	35.508187372884	38.0	34.0	40.0	27.0	41.0
35	35.35835015665316	38.0	34.0	40.0	26.0	41.0
36	35.283665802286826	38.0	34.0	40.0	25.0	41.0
37	35.23301751740501	38.0	34.0	40.0	25.0	41.0
38	35.10976175597815	38.0	34.0	40.0	25.0	41.0
39	35.022228723295555	38.0	34.0	40.0	24.0	41.0
40	34.91396864355463	38.0	34.0	40.0	24.0	41.0
41	34.79943447832292	38.0	34.0	40.0	24.0	41.0
42	34.74902969687909	38.0	34.0	40.0	23.0	41.0
43	34.709342508451094	38.0	33.0	40.0	23.0	41.0
44	34.58050015003856	37.0	33.0	40.0	23.0	41.0
45	34.46232182248986	37.0	33.0	40.0	23.0	41.0
46	34.43274841599077	37.0	33.0	40.0	23.0	41.0
47	34.386083735497614	37.0	33.0	40.0	23.0	41.0
48	34.28518812899229	37.0	33.0	40.0	23.0	41.0
49	34.194854591663876	37.0	33.0	40.0	23.0	41.0
50	34.10088743236165	36.0	33.0	40.0	23.0	41.0
51	33.52369867094877	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	4.0
11	5.0
12	8.0
13	19.0
14	51.0
15	153.0
16	384.0
17	1072.0
18	2418.0
19	4620.0
20	7674.0
21	12271.0
22	18862.0
23	27576.0
24	41345.0
25	59927.0
26	80122.0
27	92249.0
28	98953.0
29	109379.0
30	125164.0
31	148961.0
32	180190.0
33	226772.0
34	333363.0
35	426891.0
36	389535.0
37	477768.0
38	689190.0
39	1093430.0
40	446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.394927298520805	23.151003322359188	27.61464505394397	16.83942432517604
2	33.06217404257653	24.740056853320368	29.56751251127978	12.630256592823317
3	28.09003604152035	25.451379440522885	33.94007707357052	12.518507444386245
4	25.14050384991412	27.312954619520504	33.4690097777816	14.07753175278378
5	22.23016452615242	31.971119459732122	31.691025973341535	14.107690040773917
6	20.482769227790797	40.04616240087506	29.216970812929343	10.254097558404794
7	86.95731483682366	3.0814370574803633	8.39131776875993	1.5699303369360513
8	87.95505511631484	2.2835330800065825	8.047250852638474	1.7141609510401061
9	83.13717181082019	4.6158098694180545	9.641079804379835	2.60593851538191
10	36.78160301410793	30.52124148033742	18.05724266773934	14.639912837815308
11	30.26797209175261	25.150635485893684	26.973361971517413	17.60803045083629
12	28.519372182743734	22.054635546124217	29.69483555451347	19.73115671661857
13	23.78258498689448	24.930557422821565	31.18246517115689	20.104392419127066
14	20.771294988712153	27.66018363859099	31.052173623113898	20.51634774958296
15	19.72121867877874	26.16844543920427	35.369433650153105	18.74090223186389
16	22.990273844568655	24.47095543908596	33.749404416834004	18.78936629951138
17	23.317325635297674	24.13474000307606	30.04258083528993	22.50535352633634
18	23.927374884513334	25.247219446718027	31.336031517777148	19.48937415099149
19	25.315193904670124	26.509006077906044	28.7871399209044	19.388660096519427
20	26.184449552089195	26.2496060815629	28.00358371667557	19.562360649672335
21	24.708500356543244	26.678619559220056	30.054540898144793	18.55833918609191
22	24.672426569838915	22.87075925963769	30.55090501752601	21.90590915299738
23	22.610197674456124	26.178770673323577	29.92758354028616	21.283448111934142
24	22.55097815460102	25.935589898909505	31.812562583287534	19.700869363201942
25	22.624631491318738	26.385146290283203	30.446749218347513	20.54347300005055
26	21.472034210942383	28.1490404523313	29.011799806616967	21.36712553010935
27	20.698738708119613	28.028622409414893	30.933949692447843	20.33868919001765
28	19.657632445327348	27.605997670369053	32.37333035048792	20.36303953381568
29	21.460396811653748	26.205250596658715	31.429539419270114	20.904813172417427
30	22.3814937387135	26.429824438753613	31.16751509258831	20.02116672994458
31	24.124027572677278	26.60154598870032	28.62712030295958	20.64730613566282
32	24.67733105604559	26.64043770388304	28.52634171577427	20.155889524297105
33	23.657348501389066	26.66732633440207	28.534601903069714	21.14072326113915
34	21.79181531597905	27.26821193833684	29.753108594574307	21.186864151109802
35	22.051903661263488	26.93664285768063	29.201956201647523	21.80949727940836
36	24.748187975189325	27.71611199007057	27.72415706832186	19.81154296641825
37	22.315369218541107	28.803294610120233	27.88159537773686	20.999740793601795
38	22.745243132374878	28.62301172021627	27.26195226515201	21.36979288225684
39	22.469258228727597	27.602061174861063	27.508101544375386	22.42057905203595
40	23.512709180101123	26.374885588877138	28.653062453684335	21.4593427773374
41	21.40137088993838	26.300264261460743	28.985147796046512	23.313217052554368
42	22.82466139147587	27.158613880341292	27.68971811035309	22.327006617829742
43	22.131945736592524	26.773998909397147	28.517672821294937	22.576382532715396
44	22.333137225588082	27.12843408144674	28.685952626535205	21.852476066429976
45	21.465064677911847	28.416162863359506	27.496184503329353	22.62258795539929
46	22.326963596020914	27.817299284439766	28.00169075708704	21.854046362452287
47	22.50251408695353	27.355610742975884	28.406203314615265	21.735671855455326
48	22.420794161080103	27.30839430778447	28.97755444678794	21.293257084347484
49	21.757462401627944	26.798069611437782	29.387057534140492	22.057410452793782
50	21.287814825530432	27.41661566789745	29.517585702132052	21.777983804440066
51	21.07980437983525	28.1802742855422	28.395426351503232	22.34449498311932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4189.0
1	5162.5
2	6136.0
3	42084.0
4	78032.0
5	52600.0
6	27168.0
7	27470.0
8	27772.0
9	30056.5
10	32341.0
11	33254.5
12	34168.0
13	33435.0
14	32702.0
15	30794.0
16	28886.0
17	27414.0
18	25942.0
19	24733.0
20	23524.0
21	23424.5
22	23325.0
23	24460.0
24	25595.0
25	29146.5
26	37326.5
27	41955.0
28	48056.5
29	54158.0
30	64266.0
31	74374.0
32	84731.0
33	95088.0
34	111682.0
35	128276.0
36	138137.5
37	147999.0
38	165685.0
39	183371.0
40	206122.5
41	228874.0
42	248937.5
43	269001.0
44	287790.0
45	306579.0
46	317742.0
47	328905.0
48	347414.5
49	365924.0
50	368996.5
51	372069.0
52	354313.0
53	336557.0
54	310986.0
55	285415.0
56	265326.5
57	245238.0
58	227102.0
59	208966.0
60	189343.0
61	169720.0
62	151670.5
63	133621.0
64	115639.0
65	97657.0
66	81778.5
67	65900.0
68	53685.0
69	41470.0
70	34275.0
71	27080.0
72	22195.5
73	17311.0
74	13797.5
75	8033.0
76	5782.0
77	4212.5
78	2643.0
79	1856.5
80	1070.0
81	798.5
82	527.0
83	472.0
84	417.0
85	239.5
86	62.0
87	37.5
88	13.0
89	12.5
90	12.0
91	8.0
92	4.0
93	4.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4648805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.975861190011912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36945958834046	14.087585270962563
2	7.353601814521994	2.6437465092893424
3	2.6765529961345886	1.4434003537867757
4	1.4219886125936096	1.022458796550414
5	0.9269246645384511	0.8331134551670779
6	0.6793547288679649	0.7327191782945229
7	0.5233669723255219	0.6585580432172272
8	0.4434060253014525	0.6376484129307057
9	0.35237970685932785	0.570089582701233
>10	4.136999287655856	17.95378065630592
>50	1.8375747912807283	23.9229646882961
>100	1.2707500262327671	32.95492780046812
>500	0.005336421512441573	0.6657536017109045
>1k	0.002183081527816633	0.7267979113938351
>5k	0.0	0.0
>10k+	1.2128230710092409E-4	1.1464557389254044
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52586	1.131172419578795	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.604361766088274E-5	0.0	0.0	0.04269914526421306	0.0
2	8.604361766088274E-5	0.0	0.0	0.11977271578394878	0.0
3	8.604361766088274E-5	0.0	0.0	0.2209815210575621	0.0
4	8.604361766088274E-5	0.0	0.0	0.3387537227308954	0.0
5	8.604361766088274E-5	0.0	0.0	0.5771805872692014	0.0
6	8.604361766088274E-5	0.0	0.0	1.0480542849183823	0.0
7	8.604361766088274E-5	0.0	0.0	1.3153487831819144	0.0
8	8.604361766088274E-5	0.0	0.0	1.8339766886328852	0.0
9	8.604361766088274E-5	0.0	0.0	2.116027667325259	0.0
10	8.604361766088274E-5	0.0	0.0	2.516969414720557	0.0
11	8.604361766088274E-5	0.0	0.0	2.868134929299035	0.0
12	8.604361766088274E-5	0.0	0.0	3.141430969894414	0.0
13	8.604361766088274E-5	0.0	0.0	3.294502565713124	0.0
14	8.604361766088274E-5	0.0	0.0	3.383514688183307	0.0
15	8.604361766088274E-5	0.0	0.0	3.460803367747195	0.0
16	8.604361766088274E-5	0.0	0.0	3.5887072054000972	0.0
17	8.604361766088274E-5	0.0	0.0	3.725688644716223	0.0
18	8.604361766088274E-5	0.0	0.0	3.8990450233984864	0.0
19	8.604361766088274E-5	0.0	0.0	4.004405433224237	0.0
20	8.604361766088274E-5	0.0	0.0	4.115530765433268	0.0
21	8.604361766088274E-5	0.0	0.0	4.255437687749863	0.0
22	8.604361766088274E-5	0.0	0.0	4.408057554575853	0.0
23	8.604361766088274E-5	0.0	0.0	4.568098683425095	0.0
24	1.0755452207610343E-4	0.0	0.0	4.7024127705937335	0.0
25	1.0755452207610343E-4	0.0	0.0	4.818722230766832	0.0
26	1.0755452207610343E-4	0.0	0.0	4.935053201844345	0.0
27	1.0755452207610343E-4	0.0	0.0	5.058375216856805	0.0
28	1.0755452207610343E-4	0.0	0.0	5.183439615126898	0.0
29	1.0755452207610343E-4	0.0	0.0	5.319603640075245	0.0
30	1.0755452207610343E-4	0.0	0.0	5.491733897205841	0.0
31	1.505763309065448E-4	0.0	0.0	5.635061053324456	0.0
32	1.505763309065448E-4	0.0	0.0	5.782367726759888	0.0
33	1.505763309065448E-4	0.0	0.0	5.928943029445201	0.0
34	1.505763309065448E-4	0.0	0.0	6.0789385659325355	0.0
35	1.7208723532176549E-4	0.0	0.0	6.244787638973887	0.0
36	1.7208723532176549E-4	0.0	0.0	6.393922739284612	0.0
37	1.7208723532176549E-4	0.0	0.0	6.556244024001868	0.0
38	1.7208723532176549E-4	0.0	0.0	6.741560465538994	0.0
39	1.7208723532176549E-4	0.0	0.0	7.01711515109797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	26060	0.0	42.876057	1
TCGATAG	175	0.0	39.857143	1
CGGTCTA	660	0.0	38.522728	31
CACGACG	675	0.0	38.0	26
GGCGATA	1850	0.0	37.7027	8
GCGATAT	450	0.0	37.500004	9
GGGCGAT	7410	0.0	37.287453	7
TATGGGC	2125	0.0	37.164703	4
TACGGGA	1605	0.0	37.149532	4
TAATCCG	85	0.0	37.058823	1
TCTCACG	700	0.0	36.64286	23
ATAGGGC	2925	0.0	36.53846	4
AGGGCGA	3870	0.0	36.39535	6
GTAGGGC	2630	0.0	36.359314	4
TAGGGAC	4590	0.0	36.32353	5
TAGGGCA	3660	0.0	36.270493	5
TTAGGGA	5575	0.0	36.201794	4
ATAGGGA	5445	0.0	36.15703	4
GTTTTTT	32095	0.0	36.131798	2
TAGGGAT	5590	0.0	36.0644	5
>>END_MODULE