FastQCFastQC Report
Thu 26 May 2016
SRR1547443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547443_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1188064
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216591.8230499367037467No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25330.21320400247798096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18470.1554630053599806No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17260.1452783688420826No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT15960.1343361973765723No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT12960.10908503245616397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG302.1655633E-645.0000041
AGCGTAT253.8908845E-545.00000431
CGTTCCC302.1655633E-645.00000411
CCCACGT253.8908845E-545.00000414
TGTTGCG302.1655633E-645.0000041
TACGCTT253.8908845E-545.00000437
CTAAACG253.8908845E-545.0000041
ACTCACG253.8908845E-545.0000041
GCATACC253.8908845E-545.0000049
CGGGTAC551.8189894E-1245.06
CGAACTG207.0333836E-445.025
ATTAGCG406.8157533E-945.01
GATCGAC207.0333836E-445.09
CGTGCAC207.0333836E-445.018
ATTACGC207.0333836E-445.027
TAGCGCA207.0333836E-445.014
TGACCGT207.0333836E-445.014
AGCGCAT207.0333836E-445.020
TTACGCG207.0333836E-445.034
TTACGCA207.0333836E-445.028