##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547443_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1188064
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228631622538852	31.0	31.0	33.0	30.0	34.0
2	31.614988754814554	31.0	31.0	34.0	30.0	34.0
3	31.654447066824684	31.0	31.0	34.0	30.0	34.0
4	35.46616512241765	37.0	35.0	37.0	33.0	37.0
5	35.34574568373421	37.0	35.0	37.0	33.0	37.0
6	35.41206365987018	37.0	35.0	37.0	33.0	37.0
7	35.875868640073264	37.0	35.0	37.0	35.0	37.0
8	35.92229122336844	37.0	35.0	37.0	35.0	37.0
9	37.66389268591591	39.0	37.0	39.0	35.0	39.0
10	36.95360098445875	39.0	37.0	39.0	32.0	39.0
11	36.636625636329356	39.0	35.0	39.0	32.0	39.0
12	35.9637999299701	37.0	35.0	39.0	31.0	39.0
13	35.7082168974062	37.0	35.0	39.0	30.0	39.0
14	36.77795556468338	39.0	35.0	41.0	31.0	41.0
15	37.02744885797398	39.0	35.0	41.0	32.0	41.0
16	37.13722745575996	39.0	35.0	41.0	32.0	41.0
17	37.09865882646053	38.0	35.0	41.0	32.0	41.0
18	36.966894881083846	38.0	35.0	40.0	32.0	41.0
19	36.83254269130283	38.0	35.0	40.0	32.0	41.0
20	36.62313983085086	38.0	35.0	40.0	31.0	41.0
21	36.534245629865055	38.0	35.0	40.0	31.0	41.0
22	36.39738768281843	38.0	35.0	40.0	31.0	41.0
23	36.35349358283729	38.0	35.0	40.0	31.0	41.0
24	36.213957328898104	37.0	35.0	40.0	31.0	41.0
25	36.07108034583995	37.0	35.0	40.0	31.0	41.0
26	35.8813767608479	37.0	35.0	40.0	30.0	41.0
27	35.75448629030086	37.0	34.0	40.0	30.0	41.0
28	35.67310599428987	36.0	34.0	40.0	30.0	41.0
29	35.63421330837396	36.0	34.0	40.0	30.0	41.0
30	35.54245478358068	36.0	34.0	40.0	30.0	41.0
31	35.34171138928543	36.0	34.0	40.0	29.0	41.0
32	35.10647406200339	36.0	34.0	40.0	27.0	41.0
33	34.93682074366364	36.0	34.0	40.0	27.0	41.0
34	34.77626121151722	36.0	34.0	40.0	25.0	41.0
35	34.61242744498613	36.0	34.0	40.0	25.0	41.0
36	34.488072191397094	36.0	33.0	40.0	24.0	41.0
37	34.409017527675275	36.0	33.0	40.0	24.0	41.0
38	34.33103688016807	35.0	33.0	40.0	24.0	41.0
39	34.27851698225012	36.0	33.0	40.0	23.0	41.0
40	34.1382930549196	35.0	33.0	40.0	23.0	41.0
41	34.05379339833544	35.0	33.0	40.0	23.0	41.0
42	33.99675270019124	35.0	33.0	40.0	23.0	41.0
43	33.93454308858782	35.0	33.0	40.0	23.0	41.0
44	33.780282880383545	35.0	33.0	40.0	22.0	41.0
45	33.63344903978237	35.0	33.0	40.0	23.0	41.0
46	33.57356758558461	35.0	33.0	40.0	23.0	41.0
47	33.510914395184095	35.0	33.0	40.0	22.0	41.0
48	33.43935848573814	35.0	33.0	39.0	22.0	41.0
49	33.38543462305061	35.0	33.0	39.0	22.0	41.0
50	33.24755484553021	35.0	32.0	39.0	21.0	41.0
51	32.717604438818114	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	3.0
13	9.0
14	16.0
15	40.0
16	82.0
17	243.0
18	588.0
19	1189.0
20	2130.0
21	3465.0
22	5557.0
23	8236.0
24	12177.0
25	17940.0
26	22545.0
27	24938.0
28	26191.0
29	29417.0
30	34392.0
31	41467.0
32	50885.0
33	65167.0
34	105210.0
35	162571.0
36	112628.0
37	105345.0
38	134989.0
39	220489.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.08973927330514	24.981482479058368	28.256811080884532	15.67196716675196
2	33.498111212863954	25.316817949201393	28.708722762410105	12.476348075524552
3	26.439568912112478	25.8988573006168	34.933050744741024	12.728523042529696
4	24.53310596062165	27.348695019796914	33.654500094271015	14.46369892531042
5	21.62324588574353	31.47322029789641	32.80521924744795	14.098314568912112
6	19.62444784119374	41.09593422576562	29.548744848762354	9.730873084278288
7	84.84071565168206	4.030001750747434	9.335103159425755	1.7941794381447465
8	85.20820427182375	3.645426509009616	8.99345489805263	2.152914321114014
9	80.59010289007999	5.9533829827349365	10.909765803862419	2.546748323322649
10	44.51384773884235	27.449699679478545	16.958008996148354	11.078443585530746
11	37.53358404934414	24.909685000134672	23.809744256201686	13.7469866943195
12	33.87031338379077	25.239633555094677	26.456318851509682	14.43373420960487
13	20.16591698763703	38.51231920165917	26.68231677754734	14.639447033156463
14	15.307929539149406	40.9736344170011	27.92021305249549	15.798222991354002
15	14.384494438010073	29.51280402402564	41.3115791741859	14.791122363778383
16	15.00802987044469	26.78045963853799	41.62174764995825	16.589762841059066
17	15.70353112290247	27.988138686131386	28.124158294502656	28.18417189646349
18	19.229940474587227	30.52167223314569	31.83077679316939	18.41761049909769
19	24.353570178037547	33.09072575214803	24.607596897136855	17.948107172677567
20	26.579292024672075	29.80378161445848	25.002272604842833	18.614653756026613
21	20.390652355428664	33.87527944622512	27.620313383790773	18.113754814555445
22	20.97597435828373	30.902375629595713	25.290388396584696	22.831261615535865
23	18.184373905782852	35.27899170415062	25.022052684030488	21.514581706036036
24	17.97672515958736	31.70738276725833	34.4201154146578	15.895776658496514
25	16.90338230937054	33.05587914455787	31.752666523015595	18.288072023055996
26	15.695871602876613	39.2603428771514	26.53333490451693	18.51045061545506
27	16.187595954426698	38.87029654968083	28.006572036523288	16.93553545936919
28	14.477923748215584	35.3827739919735	34.067104129070486	16.072198130740432
29	15.377959436528672	31.847863414765538	33.67613192555283	19.098045223152962
30	17.168603711584563	36.810222344924185	29.10870121474937	16.912472728741886
31	24.400116492040834	34.986751555471756	23.806714116411236	16.80641783607617
32	24.992340479974143	34.82893177471921	24.51930199046516	15.659425754841491
33	22.799360977186414	35.336732701268616	23.62860923317262	18.23529708837234
34	18.904873811511838	34.96579308858782	25.44559889029547	20.68373420960487
35	18.55219920812347	33.750117838769626	28.202521076305654	19.49516187680125
36	25.295270288469307	32.789563525197295	25.085601449080187	16.82956473725321
37	19.060252646322084	37.23536779163412	26.839715705551214	16.86466385649258
38	18.60329073181243	38.0458460150295	24.492619926199264	18.858243326958817
39	18.418368034045304	36.37657567269103	26.29967745845342	18.905378834810247
40	22.18609435181943	33.10907493199019	25.221284375252512	19.483546340937863
41	17.434750989845664	31.1985717941121	27.025648449915156	24.341028766127078
42	19.925525897594742	32.32519460231099	24.155601045061545	23.593678455032727
43	19.906166671155763	33.33843968001724	24.738482101974306	22.016911546852693
44	18.411381878417323	36.304020658819724	25.95567242168772	19.32892504107523
45	17.05404759339564	40.14379696716675	22.3922280281197	20.40992741131791
46	20.775985132114094	37.65941902119751	24.001737280146525	17.56285856654187
47	19.54642174158968	33.884285695046735	25.769487165674576	20.799805397689013
48	19.75280792953915	32.25794233307296	28.928407897217657	19.060841840170227
49	18.762793923559673	31.887002720392164	28.262029654968085	21.088173701080077
50	17.622956339052443	36.22641541196434	27.6982553128451	18.45237293613812
51	16.57873649904382	37.19067322972499	25.256636006141083	20.973954265090097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2204.0
1	2136.5
2	2069.0
3	13835.5
4	25602.0
5	16415.5
6	7229.0
7	8425.5
8	9622.0
9	12746.5
10	15871.0
11	18417.5
12	20964.0
13	21375.5
14	21787.0
15	19963.0
16	18139.0
17	15769.0
18	13399.0
19	12042.0
20	10685.0
21	10494.5
22	10304.0
23	9755.0
24	9206.0
25	9242.5
26	9987.5
27	10696.0
28	13092.0
29	15488.0
30	18076.0
31	20664.0
32	24522.0
33	28380.0
34	31309.5
35	34239.0
36	35851.0
37	37463.0
38	43882.5
39	50302.0
40	60979.5
41	71657.0
42	86059.5
43	100462.0
44	102876.0
45	105290.0
46	107777.5
47	110265.0
48	107755.5
49	105246.0
50	95826.0
51	86406.0
52	73355.0
53	60304.0
54	52877.0
55	45450.0
56	41078.0
57	36706.0
58	31840.5
59	26975.0
60	24712.0
61	22449.0
62	19300.0
63	16151.0
64	13364.0
65	10577.0
66	8118.0
67	5659.0
68	4856.0
69	4053.0
70	3336.0
71	2619.0
72	1988.5
73	1358.0
74	1212.5
75	867.5
76	668.0
77	622.0
78	576.0
79	451.5
80	327.0
81	217.0
82	107.0
83	71.5
84	36.0
85	36.5
86	37.0
87	25.5
88	14.0
89	8.5
90	3.0
91	4.5
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1188064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.095885380870968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9719839623493	19.065842016764854
2	8.705677813980248	4.369533851648792
3	3.698759947334708	2.784709670690045
4	2.193057843789642	2.2014691312545955
5	1.43229648512267	1.797237421103144
6	0.9643987598891235	1.4521464443778926
7	0.7209987503485246	1.2665871398948443
8	0.5289105551331101	1.0618782934682693
9	0.4197671320548514	0.9480985049434929
>10	3.660029687405501	19.979926526710432
>50	1.077304970468421	19.846634217423667
>100	0.6203590960532089	21.872152702693352
>500	0.004416576258866894	0.7399708160939531
>1k	0.0016986831764872672	0.7671687294865065
>5k	0.0	0.0
>10k+	3.397366352974534E-4	1.8466445334461266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21659	1.8230499367037467	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2533	0.21320400247798096	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1847	0.1554630053599806	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1726	0.1452783688420826	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	1596	0.1343361973765723	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	1296	0.10908503245616397	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.417054973469443E-5	0.0	0.0	0.07474344816440866	0.0
2	8.417054973469443E-5	0.0	0.0	0.22145271635198105	0.0
3	8.417054973469443E-5	0.0	0.0	0.3731280469739004	0.0
4	8.417054973469443E-5	0.0	0.0	0.593318205079861	0.0
5	8.417054973469443E-5	0.0	0.0	1.080076494195599	0.0
6	8.417054973469443E-5	0.0	0.0	1.8966991677216043	0.0
7	8.417054973469443E-5	0.0	0.0	2.4540765480647506	0.0
8	8.417054973469443E-5	0.0	0.0	3.4017527675276753	0.0
9	8.417054973469443E-5	0.0	0.0	3.8694043418536377	0.0
10	8.417054973469443E-5	0.0	0.0	4.47905163358203	0.0
11	8.417054973469443E-5	0.0	0.0	5.09854687962938	0.0
12	8.417054973469443E-5	0.0	0.0	5.570743663641016	0.0
13	8.417054973469443E-5	0.0	0.0	5.803643574756915	0.0
14	8.417054973469443E-5	0.0	0.0	5.912812777762814	0.0
15	8.417054973469443E-5	0.0	0.0	6.032924152234223	0.0
16	8.417054973469443E-5	0.0	0.0	6.268601691491368	0.0
17	8.417054973469443E-5	0.0	0.0	6.528772860721308	0.0
18	8.417054973469443E-5	0.0	0.0	6.85316615939882	0.0
19	8.417054973469443E-5	0.0	0.0	7.026978344600964	0.0
20	8.417054973469443E-5	0.0	0.0	7.215352034907211	0.0
21	8.417054973469443E-5	0.0	0.0	7.460877528483314	0.0
22	8.417054973469443E-5	0.0	0.0	7.704719611064724	0.0
23	8.417054973469443E-5	0.0	0.0	7.983155789587093	0.0
24	8.417054973469443E-5	0.0	0.0	8.199642443504727	0.0
25	8.417054973469443E-5	0.0	0.0	8.380861637083525	0.0
26	8.417054973469443E-5	0.0	0.0	8.548866054353974	0.0
27	8.417054973469443E-5	0.0	0.0	8.723351603953995	0.0
28	8.417054973469443E-5	0.0	0.0	8.897921324103752	0.0
29	8.417054973469443E-5	0.0	0.0	9.089072642551242	0.0
30	8.417054973469443E-5	0.0	0.0	9.33198884908557	0.0
31	8.417054973469443E-5	0.0	0.0	9.547633797505858	0.0
32	8.417054973469443E-5	0.0	0.0	9.746276294879737	0.0
33	8.417054973469443E-5	0.0	0.0	9.960658685054003	0.0
34	8.417054973469443E-5	0.0	0.0	10.166287338055863	0.0
35	8.417054973469443E-5	0.0	0.0	10.410634443935681	0.0
36	8.417054973469443E-5	0.0	0.0	10.638652463166967	0.0
37	1.6834109946938885E-4	0.0	0.0	10.861451908314704	0.0
38	1.6834109946938885E-4	0.0	0.0	11.089469927545991	0.0
39	1.6834109946938885E-4	0.0	0.0	11.323127373609502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.1655633E-6	45.000004	1
AGCGTAT	25	3.8908845E-5	45.000004	31
CGTTCCC	30	2.1655633E-6	45.000004	11
CCCACGT	25	3.8908845E-5	45.000004	14
TGTTGCG	30	2.1655633E-6	45.000004	1
TACGCTT	25	3.8908845E-5	45.000004	37
CTAAACG	25	3.8908845E-5	45.000004	1
ACTCACG	25	3.8908845E-5	45.000004	1
GCATACC	25	3.8908845E-5	45.000004	9
CGGGTAC	55	1.8189894E-12	45.0	6
CGAACTG	20	7.0333836E-4	45.0	25
ATTAGCG	40	6.8157533E-9	45.0	1
GATCGAC	20	7.0333836E-4	45.0	9
CGTGCAC	20	7.0333836E-4	45.0	18
ATTACGC	20	7.0333836E-4	45.0	27
TAGCGCA	20	7.0333836E-4	45.0	14
TGACCGT	20	7.0333836E-4	45.0	14
AGCGCAT	20	7.0333836E-4	45.0	20
TTACGCG	20	7.0333836E-4	45.0	34
TTACGCA	20	7.0333836E-4	45.0	28
>>END_MODULE