FastQCFastQC Report
Thu 26 May 2016
SRR1547442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547442_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3124050
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275890.8831164674060914No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54210.17352475152446345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36200.1158752260687249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA302.1667038E-645.00000434
TCCGATT302.1667038E-645.00000432
CTACGAT302.1667038E-645.00000433
CACTACG302.1667038E-645.0000041
AGCGTGA453.8562575E-1045.041
AACCGGT406.8212103E-945.015
ACTATCG351.2128294E-745.028
ACGCCGA900.045.012
GACACGT207.0351496E-445.09
ACCGCTC900.045.018
TCGATTG207.0351496E-445.01
CCGTCGC406.8212103E-945.022
ATATCGT207.0351496E-445.017
CCCGTAG502.1827873E-1145.045
CACTCGA253.8923507E-545.023
CTACGCG700.045.01
TATTACG453.8562575E-1045.01
GCGATCG207.0351496E-445.09
ACTCGAT207.0351496E-445.029
CCGACGG207.0351496E-445.041