##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547442_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3124050
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28883884700949	31.0	31.0	33.0	30.0	34.0
2	31.698420319777213	31.0	31.0	34.0	30.0	34.0
3	31.81461564315552	31.0	31.0	34.0	30.0	34.0
4	35.55338134792977	37.0	35.0	37.0	33.0	37.0
5	35.432710423968885	37.0	35.0	37.0	33.0	37.0
6	35.48362990349066	37.0	35.0	37.0	33.0	37.0
7	35.93292969062595	37.0	35.0	37.0	35.0	37.0
8	35.982017893439604	37.0	35.0	37.0	35.0	37.0
9	37.770538883820684	39.0	38.0	39.0	35.0	39.0
10	36.97518605656119	39.0	37.0	39.0	32.0	39.0
11	36.691257822378006	39.0	35.0	39.0	32.0	39.0
12	36.025004081240695	37.0	35.0	39.0	32.0	39.0
13	35.697777244282264	38.0	35.0	39.0	30.0	39.0
14	36.769947664089884	39.0	35.0	41.0	31.0	41.0
15	37.05333429362526	39.0	35.0	41.0	32.0	41.0
16	37.181955794561546	39.0	35.0	41.0	32.0	41.0
17	37.13549399017301	39.0	35.0	41.0	32.0	41.0
18	37.04561482690738	38.0	35.0	41.0	32.0	41.0
19	36.9737398569165	38.0	35.0	41.0	32.0	41.0
20	36.817458107264606	38.0	35.0	41.0	32.0	41.0
21	36.73155071141627	38.0	35.0	41.0	31.0	41.0
22	36.63200813047166	38.0	35.0	41.0	31.0	41.0
23	36.57623661593124	38.0	35.0	40.0	31.0	41.0
24	36.48941886333446	38.0	35.0	40.0	31.0	41.0
25	36.399703589891324	38.0	35.0	40.0	31.0	41.0
26	36.24866695475424	38.0	35.0	40.0	31.0	41.0
27	36.16305596901458	38.0	35.0	40.0	30.0	41.0
28	36.12403002512764	38.0	35.0	40.0	30.0	41.0
29	36.15374913973848	38.0	35.0	40.0	30.0	41.0
30	36.09996735007442	38.0	35.0	40.0	30.0	41.0
31	35.97538131592004	38.0	35.0	40.0	30.0	41.0
32	35.8357174180951	38.0	34.0	40.0	30.0	41.0
33	35.74182071349691	38.0	35.0	40.0	30.0	41.0
34	35.66584657735952	38.0	35.0	40.0	29.0	41.0
35	35.555599942382486	38.0	34.0	40.0	29.0	41.0
36	35.46745058497783	38.0	34.0	40.0	28.0	41.0
37	35.41253501064323	38.0	34.0	40.0	28.0	41.0
38	35.34062771082409	37.0	34.0	40.0	27.0	41.0
39	35.28971591363774	37.0	34.0	40.0	27.0	41.0
40	35.20507706342728	37.0	34.0	40.0	27.0	41.0
41	35.147850386517504	37.0	34.0	40.0	27.0	41.0
42	35.11422896560554	37.0	34.0	40.0	27.0	41.0
43	35.089588514908534	37.0	34.0	40.0	27.0	41.0
44	34.96196827835662	37.0	34.0	40.0	26.0	41.0
45	34.83151870168531	37.0	34.0	40.0	26.0	41.0
46	34.819071077607596	37.0	34.0	40.0	26.0	41.0
47	34.785759190794	36.0	34.0	40.0	26.0	41.0
48	34.735293289159905	36.0	34.0	40.0	26.0	41.0
49	34.69176997807333	36.0	34.0	40.0	26.0	41.0
50	34.563090859621326	36.0	34.0	40.0	26.0	41.0
51	34.01579264096285	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	4.0
13	8.0
14	20.0
15	79.0
16	202.0
17	486.0
18	1208.0
19	2292.0
20	4170.0
21	6865.0
22	10600.0
23	16213.0
24	23642.0
25	33785.0
26	43709.0
27	51235.0
28	58475.0
29	67609.0
30	80802.0
31	99226.0
32	124003.0
33	161039.0
34	267802.0
35	413997.0
36	242428.0
37	273178.0
38	393281.0
39	747192.0
40	496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.214145100110436	25.153246586962435	29.224276179958707	13.408332132968424
2	30.97850546566156	25.69036987244122	30.958531393543637	12.37259326835358
3	28.601942990669166	24.9488324450633	33.00401722123525	13.445207343032282
4	24.682479473760022	28.38171604167667	31.922984587314545	15.012819897248765
5	22.06779661016949	32.43369984475281	31.254237288135595	14.24426625694211
6	19.60147884957027	41.61687553016117	28.21596965477505	10.56567596549351
7	88.82719546742209	3.529809061954834	6.272114722875754	1.370880747747315
8	89.68412797490437	2.645636273427122	5.8260591219730795	1.8441766296954276
9	86.46161233014837	4.231942510523199	7.301803748339496	2.0046414109889406
10	43.7019573950481	28.60392759398857	15.52526368015877	12.168851330804564
11	36.287671452121444	26.25255677726029	23.271906659624523	14.18786511099374
12	33.29194475120437	25.46719802820057	26.23360701653303	15.007250204062034
13	20.328483859093165	37.15401482050543	27.045053696323684	15.47244762407772
14	15.76107936812791	39.734191194123014	28.731486371857045	15.773243065892032
15	14.355019926057523	28.93017077191466	42.294329476160755	14.420479825867064
16	15.55119156223492	25.459963829004018	41.74501048318689	17.243834125574175
17	16.395544245450616	26.377938893423604	28.555848978089337	28.670667883036444
18	19.88175605384037	27.988220419007376	33.52535970935164	18.604663817800613
19	24.976872969382693	29.64283542196828	26.605592099998397	18.77469950865063
20	26.30649317392487	28.102783246106817	27.291208527392328	18.29951505257598
21	20.687825098830046	31.649845553048127	29.05443254749444	18.60789680062739
22	21.76965797602471	27.626830556489175	27.494342280053136	23.10916918743298
23	17.87884316832317	32.15041372577264	27.65045373793633	22.320289367967863
24	17.759734959427668	28.960708055248794	36.379283302123845	16.900273683199693
25	16.3783230101951	29.95137721867448	34.31100654598998	19.35929322514044
26	15.827371520942366	36.84659336438278	27.959795777916487	19.366239336758376
27	16.23911269025784	36.687312943134714	29.709799779132855	17.36377458747459
28	14.723643987772284	33.25750228069333	35.33486980041933	16.68398393111506
29	15.471967478113346	29.37148893263552	34.623421520142124	20.53312206910901
30	17.561626734527298	33.312494998479536	31.31243097901762	17.813447287975546
31	24.235431571197644	31.022518845729103	26.434948224260175	18.307101358813078
32	24.517949456634817	31.76572077911685	27.064835710055856	16.651494054192476
33	23.61008946719803	31.221395304172468	25.911269025783838	19.257246202845664
34	18.180854979913892	31.55762551815752	28.04433347737712	22.217186024551463
35	18.014212320545443	30.34343240345065	30.064851714921335	21.57750356108257
36	24.135945327379524	30.144780013123988	27.308877898881263	18.410396760615228
37	18.519838030761353	34.49746322882156	29.39008658632224	17.592612154094844
38	18.590611545909958	35.70435812486996	25.349754325314894	20.35527600390519
39	18.98977289095885	33.907075751028316	27.85112274131336	19.252028616699477
40	21.298442726588885	30.490613146396505	26.627742833821483	21.58320129319313
41	16.7143611657944	28.855268001472446	29.020054096445318	25.41031673628783
42	20.047342392087195	30.692498519549943	26.12800691410189	23.132152174260977
43	19.710568012675854	30.498615579136057	27.1815431891295	22.609273219058593
44	18.445991581440758	33.24741921544149	27.977977305100747	20.328611898016995
45	16.813175205262397	37.38554120452618	25.535282725948687	20.266000864262736
46	20.833469374689905	34.10217506121861	26.134440870024488	18.929914694066994
47	18.83414157904003	31.223667995070503	28.214145100110432	21.728045325779036
48	19.576351210768074	30.093788511707558	30.078167762999954	20.251692514524418
49	19.32264208319329	28.76487252124646	31.103951601286788	20.80853379427346
50	18.410236711960437	33.36521502536771	28.534402458347337	19.690145804324516
51	16.839807301419633	34.650213664954144	26.453609897408814	22.05636913621741
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4599.0
1	5079.5
2	5560.0
3	20952.0
4	36344.0
5	23901.0
6	11458.0
7	13259.5
8	15061.0
9	18789.5
10	22518.0
11	24832.5
12	27147.0
13	27678.0
14	28209.0
15	26494.5
16	24780.0
17	21975.5
18	19171.0
19	19640.0
20	20109.0
21	18357.0
22	16605.0
23	18312.0
24	20019.0
25	23715.5
26	32929.0
27	38446.0
28	49274.5
29	60103.0
30	69109.0
31	78115.0
32	90809.5
33	103504.0
34	113247.5
35	122991.0
36	125036.5
37	127082.0
38	144125.5
39	161169.0
40	193755.5
41	226342.0
42	259300.5
43	292259.0
44	292089.0
45	291919.0
46	290415.5
47	288912.0
48	277055.5
49	265199.0
50	247628.0
51	230057.0
52	199222.5
53	168388.0
54	143710.0
55	119032.0
56	105293.0
57	91554.0
58	78366.5
59	65179.0
60	55037.5
61	44896.0
62	37292.5
63	29689.0
64	23176.0
65	16663.0
66	13062.5
67	9462.0
68	7601.0
69	5740.0
70	4570.5
71	3401.0
72	2826.0
73	2251.0
74	1625.0
75	939.5
76	880.0
77	635.5
78	391.0
79	305.0
80	219.0
81	175.0
82	131.0
83	82.5
84	34.0
85	27.0
86	20.0
87	17.0
88	14.0
89	12.0
90	10.0
91	7.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3124050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.00600887462913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45722762535041	12.237750639008981
2	9.422939171230674	3.016472959996172
3	3.5784652359411764	1.718308389720789
4	1.9521250999055717	1.2498292669389943
5	1.2044041873098366	0.9638852055360864
6	0.843315873709474	0.8098872815225675
7	0.6033911963730406	0.6760519390813983
8	0.46730884084965807	0.5983799563065823
9	0.36861675706034897	0.5310074776360459
>10	2.757056405674846	9.39804901919362
>50	0.7169025605417917	8.335887479589308
>100	1.6031347804066813	56.29153345455964
>500	0.02085938194829993	2.176705875383744
>1k	0.003847847155511638	0.9262288526338416
>5k	2.0251827134271777E-4	0.17572221635500998
>10k+	2.0251827134271777E-4	0.8942999865372386
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27589	0.8831164674060914	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5421	0.17352475152446345	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3620	0.1158752260687249	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.602919287463389E-5	0.0	0.0	0.0629311310638434	0.0
2	9.602919287463389E-5	0.0	0.0	0.2190425889470399	0.0
3	9.602919287463389E-5	0.0	0.0	0.3375746226852963	0.0
4	9.602919287463389E-5	0.0	0.0	0.5467262047662489	0.0
5	9.602919287463389E-5	0.0	0.0	0.9521614570829532	0.0
6	9.602919287463389E-5	0.0	0.0	1.4122693298762823	0.0
7	9.602919287463389E-5	0.0	0.0	1.6928666314559626	0.0
8	9.602919287463389E-5	0.0	0.0	2.3077095437012853	0.0
9	9.602919287463389E-5	0.0	0.0	2.521534546502137	0.0
10	9.602919287463389E-5	0.0	0.0	2.941534226404827	0.0
11	9.602919287463389E-5	0.0	0.0	3.4875882268209537	0.0
12	9.602919287463389E-5	0.0	0.0	3.8825562971143226	0.0
13	9.602919287463389E-5	0.0	0.0	4.039756085850098	0.0
14	9.602919287463389E-5	0.0	0.0	4.099198156239497	0.0
15	9.602919287463389E-5	0.0	0.0	4.184632128166963	0.0
16	9.602919287463389E-5	0.0	0.0	4.406651622093117	0.0
17	9.602919287463389E-5	0.0	0.0	4.6643939757686335	0.0
18	9.602919287463389E-5	0.0	0.0	4.984395256157872	0.0
19	9.602919287463389E-5	0.0	0.0	5.146460523999296	0.0
20	9.602919287463389E-5	0.0	0.0	5.3010355147964985	0.0
21	1.2803892383284518E-4	0.0	0.0	5.522830940605944	0.0
22	1.2803892383284518E-4	0.0	0.0	5.757302219874842	0.0
23	1.2803892383284518E-4	0.0	0.0	6.02970503032922	0.0
24	1.2803892383284518E-4	0.0	0.0	6.2263087978745535	0.0
25	1.2803892383284518E-4	0.0	0.0	6.3859733358941115	0.0
26	1.2803892383284518E-4	0.0	0.0	6.531969718794514	0.0
27	1.2803892383284518E-4	0.0	0.0	6.6808469774811545	0.0
28	1.2803892383284518E-4	0.0	0.0	6.835646036395064	0.0
29	1.2803892383284518E-4	0.0	0.0	7.012499799939182	0.0
30	1.2803892383284518E-4	0.0	0.0	7.225204462156496	0.0
31	1.2803892383284518E-4	0.0	0.0	7.433523791232535	0.0
32	1.2803892383284518E-4	0.0	0.0	7.62026856164274	0.0
33	1.2803892383284518E-4	0.0	0.0	7.804708631423953	0.0
34	1.2803892383284518E-4	0.0	0.0	7.996638978249388	0.0
35	1.2803892383284518E-4	0.0	0.0	8.216801907779965	0.0
36	1.2803892383284518E-4	0.0	0.0	8.42179222483635	0.0
37	1.2803892383284518E-4	0.0	0.0	8.634656935708456	0.0
38	1.2803892383284518E-4	0.0	0.0	8.845697091915943	0.0
39	1.2803892383284518E-4	0.0	0.0	9.055616907539893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	30	2.1667038E-6	45.000004	34
TCCGATT	30	2.1667038E-6	45.000004	32
CTACGAT	30	2.1667038E-6	45.000004	33
CACTACG	30	2.1667038E-6	45.000004	1
AGCGTGA	45	3.8562575E-10	45.0	41
AACCGGT	40	6.8212103E-9	45.0	15
ACTATCG	35	1.2128294E-7	45.0	28
ACGCCGA	90	0.0	45.0	12
GACACGT	20	7.0351496E-4	45.0	9
ACCGCTC	90	0.0	45.0	18
TCGATTG	20	7.0351496E-4	45.0	1
CCGTCGC	40	6.8212103E-9	45.0	22
ATATCGT	20	7.0351496E-4	45.0	17
CCCGTAG	50	2.1827873E-11	45.0	45
CACTCGA	25	3.8923507E-5	45.0	23
CTACGCG	70	0.0	45.0	1
TATTACG	45	3.8562575E-10	45.0	1
GCGATCG	20	7.0351496E-4	45.0	9
ACTCGAT	20	7.0351496E-4	45.0	29
CCGACGG	20	7.0351496E-4	45.0	41
>>END_MODULE