FastQCFastQC Report
Thu 26 May 2016
SRR1547441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547441_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2661159
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303311.1397665453285581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT154200.042.475681
CGACGGT3150.042.14285728
TCGTTAG403.4605E-739.3751
ACCGTTC7050.039.25531845
ATAGGGC12050.039.2116174
ACGGGAT12850.038.69655
TGATTCG950.037.89473715
CGGTCTA3450.037.82608831
GGGCGAT44700.037.8020137
TATGGGA28000.037.2053574
TAGGGAC25400.037.1161425
TAAGGGA27350.036.9378434
CACGACG3550.036.76056326
TACGGGA9750.036.692314
TAACGGG6450.036.6279073
GACCGAT12500.036.09
GTTTTTT189550.035.9074132
GTAGGGC13300.035.864664
CGGGTAT2700.035.8333366
AGGGATC26500.035.7452856
Produced by FastQC (version 0.11.5)