##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547441_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2661159
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30983755574169	31.0	31.0	33.0	30.0	34.0
2	31.721116626251945	31.0	31.0	34.0	30.0	34.0
3	31.810739230538275	31.0	31.0	34.0	30.0	34.0
4	35.61689925329527	37.0	35.0	37.0	33.0	37.0
5	35.51642836824106	37.0	35.0	37.0	33.0	37.0
6	35.56251543030687	37.0	35.0	37.0	33.0	37.0
7	35.919007470053465	37.0	35.0	37.0	35.0	37.0
8	35.95083382841837	37.0	35.0	37.0	35.0	37.0
9	37.70523745480823	39.0	37.0	39.0	35.0	39.0
10	37.05486519219633	39.0	37.0	39.0	33.0	39.0
11	36.80315155915148	39.0	37.0	39.0	32.0	39.0
12	36.41634678724571	39.0	35.0	39.0	32.0	39.0
13	36.27892809110617	39.0	35.0	39.0	32.0	39.0
14	37.379708239905995	39.0	36.0	41.0	32.0	41.0
15	37.51266497041327	40.0	36.0	41.0	32.0	41.0
16	37.56948758041139	40.0	36.0	41.0	32.0	41.0
17	37.50778739639383	39.0	35.0	41.0	32.0	41.0
18	37.36930525383865	39.0	36.0	41.0	32.0	41.0
19	37.248832933319655	39.0	36.0	41.0	32.0	41.0
20	37.07494554064601	39.0	35.0	41.0	32.0	41.0
21	36.98666408132697	39.0	35.0	41.0	31.0	41.0
22	36.926990082140904	39.0	35.0	41.0	31.0	41.0
23	36.84560824813549	38.0	35.0	41.0	31.0	41.0
24	36.7373621794113	38.0	35.0	40.0	31.0	41.0
25	36.63293737803716	38.0	35.0	40.0	31.0	41.0
26	36.50519679583219	38.0	35.0	40.0	31.0	41.0
27	36.41054630707898	38.0	35.0	40.0	31.0	41.0
28	36.2797307488955	38.0	35.0	40.0	30.0	41.0
29	36.29252479840551	38.0	35.0	40.0	30.0	41.0
30	36.22557877977227	38.0	35.0	40.0	30.0	41.0
31	36.09003182447948	38.0	35.0	40.0	30.0	41.0
32	35.937367515432186	38.0	35.0	40.0	29.0	41.0
33	35.76522973636675	38.0	35.0	40.0	29.0	41.0
34	35.551288742987545	38.0	35.0	40.0	27.0	41.0
35	35.383962401344675	38.0	34.0	40.0	26.0	41.0
36	35.30354029954618	38.0	34.0	40.0	26.0	41.0
37	35.25006397588419	38.0	34.0	40.0	25.0	41.0
38	35.13308073662641	38.0	34.0	40.0	25.0	41.0
39	35.05702703220664	38.0	34.0	40.0	25.0	41.0
40	34.95433906805268	38.0	34.0	40.0	24.0	41.0
41	34.842473147978005	38.0	34.0	40.0	24.0	41.0
42	34.797937289729774	38.0	34.0	40.0	24.0	41.0
43	34.75646550995262	38.0	34.0	40.0	24.0	41.0
44	34.632559347261854	37.0	34.0	40.0	23.0	41.0
45	34.496565969940164	37.0	33.0	40.0	23.0	41.0
46	34.47297512099052	37.0	33.0	40.0	23.0	41.0
47	34.42487051694393	37.0	33.0	40.0	23.0	41.0
48	34.33657177192344	37.0	33.0	40.0	23.0	41.0
49	34.24588985475877	36.0	33.0	40.0	23.0	41.0
50	34.1410967927884	36.0	33.0	40.0	23.0	41.0
51	33.583912874052245	35.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	4.0
12	4.0
13	7.0
14	30.0
15	69.0
16	201.0
17	487.0
18	1211.0
19	2199.0
20	3886.0
21	6339.0
22	9893.0
23	14848.0
24	22782.0
25	34244.0
26	45276.0
27	51162.0
28	54223.0
29	58223.0
30	67167.0
31	80950.0
32	99357.0
33	128451.0
34	195943.0
35	261903.0
36	221448.0
37	276897.0
38	388790.0
39	634843.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.830093955302935	23.292933642822543	27.363190249060654	16.513782152813867
2	33.75656997571359	24.85980732455295	28.482627306372898	12.900995393360562
3	27.540819620323326	25.022368073459724	33.95505492155861	13.481757384658339
4	25.356771241402715	27.44672528022565	32.43455201286356	14.761951465508075
5	22.534880478768837	31.226582102008937	31.201893611016857	15.03664380820537
6	21.23803951586508	40.03537556380509	28.733307555091596	9.993277365238228
7	85.88592414057183	3.3108882257692986	9.048726513522867	1.754461120136001
8	86.18925062350652	2.8254982133724442	8.815858052825854	2.169393110295176
9	80.9823839913361	5.243241760450991	10.719915645776897	3.0544586024360063
10	39.32527143248487	30.950386654837235	17.46599883734869	12.258343075329208
11	31.085252703803118	23.784411228340733	28.052476383410387	17.077859684445762
12	29.129037385590262	20.95834935079039	31.533703923741495	18.37890933987785
13	23.414685105249255	26.141579665100807	32.276049646037684	18.167685583612254
14	19.373025061636678	28.921608968122538	32.58595221104789	19.119413759192895
15	18.051307719681535	25.053632646527323	38.94476053478954	17.9502990990016
16	22.21858971974241	23.553797424355327	35.633383800066056	18.5942290558362
17	21.684085768644415	24.07766691129692	30.5130959856213	23.725151334437363
18	22.71641792166496	24.810280032121344	32.65265247209956	19.820649574114135
19	25.11894253594017	26.3057186737057	28.503708346626418	20.071630443727713
20	26.4532859554803	25.482806551581472	28.752058783409783	19.31184870952844
21	24.16052554544843	25.83964355380494	30.638492476398444	19.361338424348187
22	23.738115610529096	23.619858866005377	30.05077862690655	22.59124689655898
23	21.5686473450102	26.63275662972412	30.15990401174826	21.638692013517417
24	20.700980287160593	25.182636588043028	34.06451850490707	20.051864619889304
25	21.369147803644953	26.329768345296166	31.681045739844933	20.620038111213947
26	20.807137040665364	28.627338689646127	29.252442262938814	21.31308200674969
27	19.785176308518206	28.075323571421322	31.242702897496915	20.896797222563553
28	19.200581400810698	26.63737867598291	33.66506849083425	20.496971432372135
29	20.091922354132166	24.797315756029608	33.17509400979047	21.935667880047756
30	20.80702430782978	26.298804393123447	32.754112024121824	20.140059274924948
31	23.750929576173387	26.108962298006244	29.56538861450969	20.574719511310676
32	24.47527562238859	25.786583965858483	29.44735733565713	20.290783076095792
33	23.293459729388584	26.35411863778151	28.937504297939356	21.41491733489055
34	20.501518323407208	26.671649457999315	30.234382838454977	22.592449380138504
35	21.50213497201783	27.174813680805993	30.067726129855448	21.255325217320724
36	24.69811086071896	26.840936599429043	27.93410690605109	20.52684563380091
37	21.18404048762212	29.031748948484477	28.955842172527085	20.82836839136632
38	20.896571756892392	29.52788615787332	27.95980999256339	21.6157320926709
39	20.83347894657929	29.04542719920155	27.77436447803382	22.34672937618534
40	22.781464767794784	27.10608422871388	28.564321034556748	21.54812996893459
41	20.206120716574997	26.817450592016485	29.752562699184832	23.22386599222369
42	21.614191410584635	27.080418719813437	28.377785769283232	22.927604100318696
43	20.77767619296705	26.703740738527838	29.31918010160235	23.199402966902767
44	21.10050545645713	27.572009038167206	29.066433084231345	22.26105242114432
45	19.77769836375805	29.39204309099907	27.99111214324285	22.83914640200003
46	21.239617775563204	28.880649371195034	28.005729834256428	21.874003018985334
47	20.735664422907462	27.191573295695598	29.70611677092575	22.36664551047119
48	20.987509577593823	26.9572768857479	30.14573725207701	21.909476284581267
49	21.132934935492393	26.213089860470568	30.385632726191858	22.26834247784518
50	20.044424252741006	28.21184303530905	30.329642084520316	21.414090627429626
51	19.94379140817967	28.46383850044285	28.894853708478145	22.69751638289933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2607.0
1	3199.5
2	3792.0
3	24395.5
4	44999.0
5	30523.0
6	16047.0
7	16155.5
8	16264.0
9	17154.5
10	18045.0
11	18318.0
12	18591.0
13	18133.5
14	17676.0
15	17045.0
16	16414.0
17	15791.0
18	15168.0
19	14747.0
20	14326.0
21	15072.0
22	15818.0
23	16522.0
24	17226.0
25	19118.0
26	24129.5
27	27249.0
28	32206.0
29	37163.0
30	44307.0
31	51451.0
32	58642.5
33	65834.0
34	73556.0
35	81278.0
36	88777.0
37	96276.0
38	104298.5
39	112321.0
40	126992.0
41	141663.0
42	158364.5
43	175066.0
44	186047.5
45	197029.0
46	202344.5
47	207660.0
48	204672.0
49	201684.0
50	185381.0
51	169078.0
52	161598.5
53	154119.0
54	143977.0
55	133835.0
56	128649.0
57	123463.0
58	116846.5
59	110230.0
60	100491.0
61	90752.0
62	82168.0
63	73584.0
64	64937.0
65	56290.0
66	48531.5
67	40773.0
68	34730.5
69	28688.0
70	24020.5
71	19353.0
72	15798.5
73	12244.0
74	10018.5
75	5959.5
76	4126.0
77	3396.0
78	2666.0
79	1774.0
80	882.0
81	622.0
82	362.0
83	267.5
84	173.0
85	101.5
86	30.0
87	38.5
88	47.0
89	27.5
90	8.0
91	7.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2661159.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.879200879445946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58363669085627	16.19884335426341
2	7.504742763618164	3.1338606342030397
3	2.8225331709538533	1.7679671119573515
4	1.593124224574807	1.3305264284323581
5	1.0104897733290945	1.0549109481981982
6	0.7597353539862464	0.9517600242657489
7	0.5730305921279707	0.8375094590175429
8	0.43701868655608556	0.7299680755740907
9	0.3830183015518981	0.7197404452745655
>10	4.651356898749436	23.96868928431134
>50	2.047982018846961	30.496536715112022
>100	0.6280386108054796	16.722614721274137
>500	0.0036502855474776757	0.49299701996109724
>1k	0.0014601142189910703	0.4382365099754671
>5k	0.0	0.0
>10k+	1.8251427737388378E-4	1.155839268179643
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30331	1.1397665453285581	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.515522372019109E-5	0.0	0.0	0.03750245663637535	0.0
2	7.515522372019109E-5	0.0	0.0	0.10747196991987326	0.0
3	7.515522372019109E-5	0.0	0.0	0.19104457869672575	0.0
4	7.515522372019109E-5	0.0	0.0	0.31749324260594725	0.0
5	7.515522372019109E-5	0.0	0.0	0.5816638539824189	0.0
6	7.515522372019109E-5	0.0	0.0	1.0852414305195592	0.0
7	7.515522372019109E-5	0.0	0.0	1.3556123478529467	0.0
8	7.515522372019109E-5	0.0	0.0	1.9399066346655724	0.0
9	7.515522372019109E-5	0.0	0.0	2.2310203937457325	0.0
10	7.515522372019109E-5	0.0	0.0	2.654444924185289	0.0
11	7.515522372019109E-5	0.0	0.0	3.037097745756642	0.0
12	7.515522372019109E-5	0.0	0.0	3.3404618063031934	0.0
13	7.515522372019109E-5	0.0	0.0	3.510087146239665	0.0
14	7.515522372019109E-5	0.0	0.0	3.6003109923157544	0.0
15	7.515522372019109E-5	0.0	0.0	3.6892571995886003	0.0
16	1.1273283558028663E-4	0.0	0.0	3.8443775813470746	0.0
17	1.1273283558028663E-4	0.0	0.0	4.023960988426471	0.0
18	1.1273283558028663E-4	0.0	0.0	4.2547626804711784	0.0
19	1.1273283558028663E-4	0.0	0.0	4.389779039884502	0.0
20	1.1273283558028663E-4	0.0	0.0	4.533475827637507	0.0
21	1.1273283558028663E-4	0.0	0.0	4.71952258395684	0.0
22	1.1273283558028663E-4	0.0	0.0	4.915264364136077	0.0
23	1.1273283558028663E-4	0.0	0.0	5.141669475593154	0.0
24	1.1273283558028663E-4	0.0	0.0	5.312384566273567	0.0
25	1.1273283558028663E-4	0.0	0.0	5.466978861466	0.0
26	1.1273283558028663E-4	0.0	0.0	5.607105776092297	0.0
27	1.1273283558028663E-4	0.0	0.0	5.763654107101455	0.0
28	1.1273283558028663E-4	0.0	0.0	5.92681609779799	0.0
29	1.1273283558028663E-4	0.0	0.0	6.1041824257776405	0.0
30	1.1273283558028663E-4	0.0	0.0	6.338215792442315	0.0
31	1.1273283558028663E-4	0.0	0.0	6.531552605462507	0.0
32	1.1273283558028663E-4	0.0	0.0	6.720192217000187	0.0
33	1.1273283558028663E-4	0.0	0.0	6.899211959901682	0.0
34	1.1273283558028663E-4	0.0	0.0	7.08713759681402	0.0
35	1.1273283558028663E-4	0.0	0.0	7.312603267974593	0.0
36	1.1273283558028663E-4	0.0	0.0	7.503009027269697	0.0
37	1.1273283558028663E-4	0.0	0.0	7.7162995521876	0.0
38	1.5031044744038218E-4	0.0	0.0	7.9487170815422905	0.0
39	1.5031044744038218E-4	0.0	0.0	8.29048546140986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15420	0.0	42.47568	1
CGACGGT	315	0.0	42.142857	28
TCGTTAG	40	3.4605E-7	39.375	1
ACCGTTC	705	0.0	39.255318	45
ATAGGGC	1205	0.0	39.211617	4
ACGGGAT	1285	0.0	38.6965	5
TGATTCG	95	0.0	37.894737	15
CGGTCTA	345	0.0	37.826088	31
GGGCGAT	4470	0.0	37.802013	7
TATGGGA	2800	0.0	37.205357	4
TAGGGAC	2540	0.0	37.116142	5
TAAGGGA	2735	0.0	36.937843	4
CACGACG	355	0.0	36.760563	26
TACGGGA	975	0.0	36.69231	4
TAACGGG	645	0.0	36.627907	3
GACCGAT	1250	0.0	36.0	9
GTTTTTT	18955	0.0	35.907413	2
GTAGGGC	1330	0.0	35.86466	4
CGGGTAT	270	0.0	35.833336	6
AGGGATC	2650	0.0	35.745285	6
>>END_MODULE