##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547440_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1777154
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238736204065603	31.0	31.0	33.0	30.0	34.0
2	31.636410800639673	31.0	31.0	34.0	30.0	34.0
3	31.788914185264755	31.0	31.0	34.0	30.0	34.0
4	35.53223806152984	37.0	35.0	37.0	33.0	37.0
5	35.396425408265124	37.0	35.0	37.0	33.0	37.0
6	35.44185759928515	37.0	35.0	37.0	33.0	37.0
7	35.874009230488745	37.0	35.0	37.0	35.0	37.0
8	35.92086223253584	37.0	35.0	37.0	35.0	37.0
9	37.69736049886504	39.0	37.0	39.0	35.0	39.0
10	36.935046709514204	39.0	37.0	39.0	32.0	39.0
11	36.65629258916222	39.0	35.0	39.0	32.0	39.0
12	35.90387833581108	37.0	35.0	39.0	31.0	39.0
13	35.533879449952	37.0	35.0	39.0	30.0	39.0
14	36.594514037612946	38.0	35.0	41.0	31.0	41.0
15	36.871724116199275	38.0	35.0	41.0	31.0	41.0
16	36.9993641518968	38.0	35.0	41.0	32.0	41.0
17	36.93579397170982	38.0	35.0	41.0	32.0	41.0
18	36.861121208404	38.0	35.0	41.0	32.0	41.0
19	36.82225400837519	38.0	35.0	41.0	31.0	41.0
20	36.6927379394245	38.0	35.0	40.0	31.0	41.0
21	36.6031244337857	38.0	35.0	40.0	31.0	41.0
22	36.4991250054863	38.0	35.0	40.0	31.0	41.0
23	36.47827650276791	38.0	35.0	40.0	31.0	41.0
24	36.37582674320852	38.0	35.0	40.0	31.0	41.0
25	36.260976257544364	38.0	35.0	40.0	31.0	41.0
26	36.11499172272071	38.0	35.0	40.0	30.0	41.0
27	36.02807353780258	38.0	35.0	40.0	30.0	41.0
28	35.99122979775529	37.0	35.0	40.0	30.0	41.0
29	36.0731337858171	38.0	35.0	40.0	30.0	41.0
30	36.03883962785442	38.0	35.0	40.0	30.0	41.0
31	35.930205260770876	38.0	35.0	40.0	30.0	41.0
32	35.83298127230392	38.0	34.0	40.0	30.0	41.0
33	35.76414199332191	38.0	34.0	40.0	30.0	41.0
34	35.7080821358194	38.0	34.0	40.0	29.0	41.0
35	35.61307517525211	38.0	34.0	40.0	29.0	41.0
36	35.50373743637299	38.0	34.0	40.0	29.0	41.0
37	35.45441419257982	38.0	34.0	40.0	28.0	41.0
38	35.406872448870494	37.0	34.0	40.0	28.0	41.0
39	35.33800390962179	37.0	34.0	40.0	28.0	41.0
40	35.21696825373603	37.0	34.0	40.0	27.0	41.0
41	35.183861950061726	37.0	34.0	40.0	27.0	41.0
42	35.14003400943306	37.0	34.0	40.0	27.0	41.0
43	35.11037647834684	37.0	34.0	40.0	27.0	41.0
44	34.998144223854545	37.0	34.0	40.0	27.0	41.0
45	34.86313285173935	37.0	34.0	40.0	26.0	41.0
46	34.834674991587676	36.0	34.0	40.0	26.0	41.0
47	34.812992571268445	36.0	34.0	40.0	27.0	41.0
48	34.78822319281278	36.0	34.0	40.0	27.0	41.0
49	34.72023752584188	36.0	34.0	40.0	26.0	41.0
50	34.60840647462178	36.0	34.0	40.0	26.0	41.0
51	34.03972587631685	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	5.0
13	3.0
14	19.0
15	41.0
16	103.0
17	314.0
18	657.0
19	1258.0
20	2290.0
21	3796.0
22	6002.0
23	8771.0
24	13013.0
25	18533.0
26	24249.0
27	29162.0
28	33945.0
29	39569.0
30	47849.0
31	59004.0
32	73480.0
33	95218.0
34	157936.0
35	235781.0
36	130320.0
37	156700.0
38	233925.0
39	404979.0
40	231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.03017071114828	24.956475353289587	29.321882065369685	12.691471870192453
2	28.735157448369698	26.765772690492778	31.352657113564725	13.146412747572805
3	28.52099480405187	26.39253548088686	31.927002386962528	13.159467328098748
4	25.298201506453577	28.081246757456025	31.476675628561175	15.14387610752923
5	23.24677546234035	33.16538690513034	28.949657711149403	14.638179921379916
6	20.554887196044913	41.48779452990568	27.3238560079768	10.633462266072609
7	90.0009228237958	3.6387955123754048	5.019036054275544	1.3412456095532521
8	91.16143001675712	2.569389034377437	4.587447120508408	1.681733828357025
9	86.94339376328669	4.247746678115684	6.40737943926075	2.401480119336872
10	44.345003303033955	27.495253647123434	14.106430843922361	14.053312205920252
11	39.66746832294781	23.43589807073557	21.28487458036839	15.61175902594823
12	38.30951059953161	21.929782112298653	23.753034345926128	16.00767294224361
13	21.982619401582532	37.02881123414178	23.50646032926803	17.48210903500766
14	16.85700845284089	38.767433773325216	25.347606341375027	19.027951432458863
15	15.64507071418684	26.297946041817422	41.187539177809015	16.869444066186723
16	17.442607674967956	21.972322038495257	41.68321934958929	18.9018509369475
17	17.932435793409013	22.73871594695789	26.68795163502994	32.64089662460316
18	20.827176485549366	25.39386007065229	32.39404125922683	21.38492218457151
19	26.52426295076285	27.714480568369424	25.16911871452896	20.592137766338762
20	29.561647443046578	23.930734196361147	26.22569569097557	20.2819226696167
21	22.37836450864697	29.948501930614906	26.823505447473885	20.84962811326424
22	22.66157012841881	25.842667545975196	25.26016315974868	26.23559916585732
23	20.066071932989487	30.000663982975027	25.208001107388554	24.72526297664693
24	20.281585051154824	25.833664386991785	35.71502525948792	18.169725302365467
25	18.711265315217478	26.79734001667835	33.10996120763873	21.38143346046544
26	18.522761674002368	33.71418571491272	26.224514026358996	21.538538584725917
27	18.046044405830898	33.93296247820955	28.115740110311204	19.90525300564836
28	16.290484673809924	29.923236815717715	35.28934464880365	18.496933861668712
29	17.013044451972085	25.14407867860636	34.894950015586716	22.947926853834836
30	18.859648629212774	29.890487824915567	31.77293582885895	19.476927717012707
31	26.092167589302896	28.276727846883276	25.405395368099782	20.225709195714046
32	27.14036037394621	28.19631838321271	26.493933558937492	18.16938768390359
33	25.31879623262812	28.918878161374874	25.33089422751208	20.431431378484927
34	20.571149151958693	28.105105128762055	27.866071257752562	23.457674461526686
35	20.4858442205909	26.252423819207564	29.822063816641663	23.43966814355987
36	27.101365441599324	26.60292805238038	26.569785173372708	19.72592133264759
37	20.764266912152802	30.878021825908164	29.394244955698827	18.9634663062402
38	20.913438002559147	30.93260347724508	26.124128803693996	22.029829716501776
39	20.269430786527224	30.773810260675216	28.135659599561997	20.821099353235567
40	23.43404116919524	27.066984628231435	27.266066981252045	22.23290722132128
41	18.494007834999103	25.510619788718365	29.420354116750712	26.575018259531813
42	22.13246572891263	26.063582559530573	25.978277628162783	25.825674083394013
43	21.644888400217425	27.33465979875689	26.890578981900276	24.12987281912541
44	20.2697684049891	29.745368156051754	27.509489892265947	22.475373546693195
45	18.66630579004408	34.1731780138356	24.349662437807865	22.810853758312447
46	21.753995433147605	31.966672556233167	25.924933911186088	20.354398099433137
47	21.107793697113475	28.283311406889894	27.469369565046136	23.139525330950498
48	22.021951952391294	24.889683167581424	30.52785521119723	22.56050966883005
49	20.158241773082132	25.989475307148396	30.614904504618057	23.237378415151415
50	19.613156766380406	30.286964438647413	28.705784642186327	21.394094152785858
51	18.34106667176846	31.425695240817618	26.372278373174186	23.860959714239733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1367.0
1	1627.5
2	1888.0
3	6138.5
4	10389.0
5	7450.0
6	4511.0
7	4936.5
8	5362.0
9	6003.0
10	6644.0
11	7043.0
12	7442.0
13	7098.5
14	6755.0
15	6646.5
16	6538.0
17	6466.5
18	6395.0
19	6040.0
20	5685.0
21	5961.0
22	6237.0
23	6513.0
24	6789.0
25	8056.0
26	11014.0
27	12705.0
28	16311.0
29	19917.0
30	26353.0
31	32789.0
32	37652.0
33	42515.0
34	48080.0
35	53645.0
36	57289.5
37	60934.0
38	68873.5
39	76813.0
40	98092.5
41	119372.0
42	133655.0
43	147938.0
44	155718.5
45	163499.0
46	161783.0
47	160067.0
48	159326.5
49	158586.0
50	159522.0
51	160458.0
52	144538.0
53	128618.0
54	114011.5
55	99405.0
56	85826.5
57	72248.0
58	64184.5
59	56121.0
60	51510.5
61	46900.0
62	39924.5
63	32949.0
64	26139.5
65	19330.0
66	15270.5
67	11211.0
68	9022.5
69	6834.0
70	5373.0
71	3912.0
72	3268.5
73	2625.0
74	2001.0
75	923.0
76	469.0
77	367.0
78	265.0
79	264.0
80	263.0
81	146.0
82	29.0
83	27.5
84	26.0
85	15.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1777154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.249665014241536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32988595387302	12.728344073541798
2	8.80458427540984	2.861430901301369
3	3.154588803386486	1.5378303393812238
4	1.4318363693150042	0.9306744542630659
5	0.8044099190898759	0.6535695859671812
6	0.5425405183273114	0.5289661007683067
7	0.3826660475937021	0.4352736560005027
8	0.28875219715295986	0.3753701180689461
9	0.2179716614642305	0.3187769833252266
>10	2.6337616027680326	10.667045480756999
>50	1.4385619705892472	17.316867324535426
>100	1.9592210899640856	49.70875839507204
>500	0.009466529962245911	1.1161071699906235
>1k	0.0014024488832956902	0.44803812071497007
>5k	3.5061222082392255E-4	0.372947296312334
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6546	0.36834174190869223	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3444	0.1937929971178637	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2372	0.1334718319290281	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.62697436463019E-5	0.0	0.0	0.05857680313580027	0.0
2	5.62697436463019E-5	0.0	0.0	0.20881701867142632	0.0
3	5.62697436463019E-5	0.0	0.0	0.30498201056295626	0.0
4	5.62697436463019E-5	0.0	0.0	0.5421589800321188	0.0
5	5.62697436463019E-5	0.0	0.0	0.9080248532203737	0.0
6	5.62697436463019E-5	0.0	0.0	1.3247023049212392	0.0
7	5.62697436463019E-5	0.0	0.0	1.5356013041075787	0.0
8	5.62697436463019E-5	0.0	0.0	2.12215711187663	0.0
9	5.62697436463019E-5	0.0	0.0	2.3063279828309757	0.0
10	5.62697436463019E-5	0.0	0.0	2.676189007818118	0.0
11	5.62697436463019E-5	0.0	0.0	3.1355751949465267	0.0
12	5.62697436463019E-5	0.0	0.0	3.4908060865856307	0.0
13	5.62697436463019E-5	0.0	0.0	3.635306788269334	0.0
14	5.62697436463019E-5	0.0	0.0	3.689156932938845	0.0
15	5.62697436463019E-5	0.0	0.0	3.779807489953037	0.0
16	5.62697436463019E-5	0.0	0.0	3.992000693243242	0.0
17	5.62697436463019E-5	0.0	0.0	4.232835196049414	0.0
18	5.62697436463019E-5	0.0	0.0	4.546820365595779	0.0
19	5.62697436463019E-5	0.0	0.0	4.699536449851842	0.0
20	5.62697436463019E-5	0.0	0.0	4.857204271548779	0.0
21	5.62697436463019E-5	0.0	0.0	5.086109588701936	0.0
22	5.62697436463019E-5	0.0	0.0	5.309275392003169	0.0
23	5.62697436463019E-5	0.0	0.0	5.581452142020331	0.0
24	5.62697436463019E-5	0.0	0.0	5.768886658106163	0.0
25	5.62697436463019E-5	0.0	0.0	5.930380822371049	0.0
26	5.62697436463019E-5	0.0	0.0	6.086810709707769	0.0
27	5.62697436463019E-5	0.0	0.0	6.227653878054462	0.0
28	5.62697436463019E-5	0.0	0.0	6.386615903855265	0.0
29	5.62697436463019E-5	0.0	0.0	6.559476556336705	0.0
30	5.62697436463019E-5	0.0	0.0	6.769587779111996	0.0
31	1.125394872926038E-4	0.0	0.0	6.9683887834143805	0.0
32	1.125394872926038E-4	0.0	0.0	7.14276871897427	0.0
33	1.125394872926038E-4	0.0	0.0	7.3239010237717155	0.0
34	1.125394872926038E-4	0.0	0.0	7.504189282414467	0.0
35	1.125394872926038E-4	0.0	0.0	7.74136625188363	0.0
36	1.125394872926038E-4	0.0	0.0	7.940167256186014	0.0
37	1.125394872926038E-4	0.0	0.0	8.144370155878445	0.0
38	1.125394872926038E-4	0.0	0.0	8.337712995047138	0.0
39	1.125394872926038E-4	0.0	0.0	8.529649090624673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.034327E-4	45.000004	23
TCGTTGC	20	7.034327E-4	45.000004	25
CGAACCG	20	7.034327E-4	45.000004	41
GTCGATA	20	7.034327E-4	45.000004	43
CGTATCA	25	3.891668E-5	45.000004	10
GTTTCGA	40	6.8193913E-9	45.000004	21
ACGCATC	20	7.034327E-4	45.000004	33
CGTGCGT	35	1.2124292E-7	45.000004	17
TTAGCGA	20	7.034327E-4	45.000004	1
TGGCGTA	35	1.2124292E-7	45.000004	33
CAATTCG	20	7.034327E-4	45.000004	44
TGCGGAC	20	7.034327E-4	45.000004	17
CGTTCGA	25	3.891668E-5	45.000004	14
CGTTCCA	25	3.891668E-5	45.000004	41
TCTAGCG	45	3.8562575E-10	45.000004	1
ATCGTAC	25	3.891668E-5	45.000004	27
GAAACGC	25	3.891668E-5	45.000004	44
AACGCAT	20	7.034327E-4	45.000004	32
AGCGCGA	25	3.891668E-5	45.000004	1
TCGTCGT	20	7.034327E-4	45.000004	45
>>END_MODULE