FastQCFastQC Report
Thu 26 May 2016
SRR1547439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547439_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences821799
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79770.9706753111162219No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12700.15453900527987988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9150.11134109435518905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG302.164743E-645.0000042
CGTAAGG1300.045.0000042
TGCAGCG302.164743E-645.0000041
CGGACGG302.164743E-645.0000042
CGGGTTC406.8102963E-945.06
AACGTAC253.8898324E-545.011
CGGGTCA502.1827873E-1145.06
TTGGCCG253.8898324E-545.036
TACCCGG207.032112E-445.016
CGAACGG253.8898324E-545.02
ACACGCG207.032112E-445.036
GTTTACG453.8380676E-1045.01
CCGTGAC207.032112E-445.021
TTGTACG253.8898324E-545.01
TCGATAG406.8102963E-945.01
CCGTATC207.032112E-445.040
AGACGAG253.8898324E-545.01
TCGTGCG253.8898324E-545.01
ATATACG207.032112E-445.01
CGGATCG207.032112E-445.020