##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547439_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	821799
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167901153445065	31.0	31.0	33.0	30.0	34.0
2	31.571398845703147	31.0	31.0	34.0	30.0	34.0
3	31.639447115413866	31.0	31.0	34.0	30.0	34.0
4	35.4309837320318	37.0	35.0	37.0	33.0	37.0
5	35.31151291252484	37.0	35.0	37.0	33.0	37.0
6	35.37887975040125	37.0	35.0	37.0	33.0	37.0
7	35.863528673069695	37.0	35.0	37.0	35.0	37.0
8	35.90689329142528	37.0	35.0	37.0	35.0	37.0
9	37.66322665274599	39.0	37.0	39.0	35.0	39.0
10	36.795550980227524	39.0	37.0	39.0	32.0	39.0
11	36.53267648171877	39.0	35.0	39.0	32.0	39.0
12	35.94974196853489	37.0	35.0	39.0	31.0	39.0
13	35.720805209059634	37.0	35.0	39.0	30.0	39.0
14	36.78611801669265	39.0	35.0	41.0	31.0	41.0
15	37.01721832224181	39.0	35.0	41.0	32.0	41.0
16	37.1118339155925	39.0	35.0	41.0	32.0	41.0
17	37.0743308278545	38.0	35.0	41.0	32.0	41.0
18	36.95288628971318	38.0	35.0	40.0	32.0	41.0
19	36.83980876102307	38.0	35.0	40.0	31.0	41.0
20	36.65141232831873	38.0	35.0	40.0	31.0	41.0
21	36.56261689293854	38.0	35.0	40.0	31.0	41.0
22	36.45155810605756	38.0	35.0	40.0	31.0	41.0
23	36.38838329080468	38.0	35.0	40.0	31.0	41.0
24	36.290400694087	38.0	35.0	40.0	31.0	41.0
25	36.183120203358726	38.0	35.0	40.0	30.0	41.0
26	36.01865541330666	37.0	35.0	40.0	30.0	41.0
27	35.90650755233336	37.0	34.0	40.0	30.0	41.0
28	35.8239837235139	37.0	34.0	40.0	30.0	41.0
29	35.829719919347674	37.0	35.0	40.0	30.0	41.0
30	35.74789334131582	37.0	35.0	40.0	30.0	41.0
31	35.60751473292131	37.0	34.0	40.0	29.0	41.0
32	35.39332732213108	37.0	34.0	40.0	29.0	41.0
33	35.2384427335638	37.0	34.0	40.0	27.0	41.0
34	35.07275745042279	37.0	34.0	40.0	26.0	41.0
35	34.91730946374965	37.0	34.0	40.0	25.0	41.0
36	34.814833067453236	37.0	34.0	40.0	24.0	41.0
37	34.75414547839557	37.0	34.0	40.0	24.0	41.0
38	34.67439483377322	37.0	34.0	40.0	24.0	41.0
39	34.618839886638945	37.0	34.0	40.0	24.0	41.0
40	34.52388114368598	37.0	33.0	40.0	23.0	41.0
41	34.47638047746469	37.0	34.0	40.0	23.0	41.0
42	34.43226871777649	37.0	33.0	40.0	23.0	41.0
43	34.387770002153815	37.0	33.0	40.0	23.0	41.0
44	34.28084969682367	36.0	33.0	40.0	23.0	41.0
45	34.144885793241414	36.0	33.0	40.0	23.0	41.0
46	34.112632164312686	36.0	33.0	40.0	23.0	41.0
47	34.07367008234374	36.0	33.0	40.0	23.0	41.0
48	34.006202246534734	36.0	33.0	40.0	23.0	41.0
49	33.9503540403432	36.0	33.0	40.0	23.0	41.0
50	33.83418086417725	36.0	33.0	40.0	23.0	41.0
51	33.29328461095718	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	2.0
13	3.0
14	6.0
15	27.0
16	79.0
17	194.0
18	454.0
19	854.0
20	1598.0
21	2443.0
22	3807.0
23	5653.0
24	8380.0
25	12032.0
26	15217.0
27	16548.0
28	17481.0
29	19445.0
30	22906.0
31	27612.0
32	33918.0
33	43102.0
34	68105.0
35	101323.0
36	67089.0
37	75577.0
38	103550.0
39	174289.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.910478109610747	25.030938222120007	29.155791136275415	14.902792531993834
2	33.251561513216735	25.717845847950656	27.784774622505015	13.245818016327593
3	27.293048543500298	24.821032880302848	34.55127105289736	13.334647523299491
4	24.057099120344514	27.725271021259456	34.006125585453375	14.211504272942655
5	20.918740470601694	32.380180555099244	33.281495840223705	13.419583134075364
6	19.46668224225145	40.90002543200953	30.166500567657057	9.466791758081964
7	86.80054368525637	3.4529124518282455	8.335979965904071	1.410563897011313
8	87.87039166511519	2.629231722112098	7.691905198229737	1.8084714145429721
9	83.603654908317	4.503412634963051	9.5059740885545	2.386958368165452
10	42.90927586916022	22.50939706667932	20.040666878397275	14.540660185763185
11	35.51111646521838	26.644471458349305	22.103945125267856	15.740466951164459
12	30.668934861200853	24.202876859183327	28.159197078604382	16.96899120101144
13	19.916913989917244	35.35876777654877	29.875675195516177	14.848643038017812
14	17.3499846069416	37.42861697325015	29.977403233637425	15.243995186170828
15	15.788167179565807	28.03313218925796	41.31557716667945	14.863123464496795
16	16.495152707657226	25.14045405263331	41.05772822794868	17.30666501176078
17	17.294374901892066	26.127800106838777	29.22819326867032	27.349631722598833
18	20.27284043908547	27.45281997179359	33.61929133522917	18.655048253891767
19	24.43967442160431	30.246812176700143	27.162481336677217	18.15103206501833
20	25.37104571799187	28.223933102863352	27.402807742525848	19.00221343661893
21	21.122804968124807	31.420821879802723	29.26153475484881	18.194838397223652
22	21.576443874962127	27.968639533511237	27.39149110670614	23.063425484820495
23	18.065122980193454	32.4838555413185	27.40597153318512	22.045049945302928
24	18.322971918924214	29.024372139659455	35.28928606630088	17.36336987511545
25	17.340128182195404	29.904392679961887	33.70045473406515	19.055024403777566
26	17.295956797221702	35.30352312426761	28.738414137763613	18.662105940747068
27	18.210170613495514	34.850127585942545	29.201544416578752	17.73815738398319
28	16.864829477767678	31.533501500975298	34.725522907669635	16.87614611358739
29	16.44550553115786	28.860098393889505	34.48604829161388	20.20834778333875
30	18.079238353904056	32.64691244452719	30.71979888026147	18.554050321307276
31	23.901708325271752	30.09945254253169	26.786963722272723	19.211875409923838
32	23.941012340000416	29.55880939256436	27.96730100669385	18.532877260741373
33	22.881142469143914	31.04858974031363	26.591052069910038	19.479215720632418
34	17.93917977510316	31.773097801287175	28.19850109333304	22.089221330276626
35	18.655169938147893	30.526442597277438	29.79244316432607	21.0259443002486
36	24.587885845565644	29.900863836534235	26.92288503636534	18.58836528153478
37	18.31749612739855	34.46828239021951	28.9724129622937	18.241808520088245
38	18.594084441572694	35.717127910839515	25.572189793367965	20.11659785421983
39	18.74558134044943	33.62379365270583	28.34659083303825	19.284034173806493
40	20.543344540453322	31.8141053956016	26.52923646779809	21.11331359614699
41	17.562323633881277	29.097747746103366	28.477279724117455	24.8626488958979
42	20.137405862017353	30.563921348164207	26.542256683203558	22.75641610661488
43	19.49211425178176	30.484096476145627	28.180005086401906	21.843784185670707
44	18.541030105901807	33.0173193201744	28.330406826973505	20.11124374695029
45	17.48590592103422	36.482156829102976	25.51694514108681	20.51499210877599
46	20.957071011281347	34.09422498688852	25.834784418087636	19.113919583742497
47	19.751910138610537	31.180008736929587	27.136197537354022	21.93188358710585
48	20.41204722809349	29.7455947257176	29.147881659627235	20.69447638656168
49	19.31250828973995	29.0781565808671	30.04773673367819	21.561598395714768
50	18.549061266806117	33.20641665419403	28.366060314018394	19.878461764981463
51	17.570719847553963	34.17855217638376	26.30034838202529	21.950379594036985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1069.0
1	1050.0
2	1031.0
3	6008.0
4	10985.0
5	7583.5
6	4182.0
7	4610.5
8	5039.0
9	6182.5
10	7326.0
11	8075.0
12	8824.0
13	9278.5
14	9733.0
15	9292.0
16	8851.0
17	8164.0
18	7477.0
19	7014.5
20	6552.0
21	6024.0
22	5496.0
23	5860.0
24	6224.0
25	6396.0
26	7916.5
27	9265.0
28	10355.0
29	11445.0
30	13057.5
31	14670.0
32	16771.5
33	18873.0
34	21838.5
35	24804.0
36	27287.5
37	29771.0
38	33228.0
39	36685.0
40	46364.0
41	56043.0
42	64631.5
43	73220.0
44	77384.5
45	81549.0
46	80976.5
47	80404.0
48	75254.0
49	70104.0
50	65799.0
51	61494.0
52	54616.0
53	47738.0
54	41738.0
55	35738.0
56	32419.0
57	29100.0
58	24420.5
59	19741.0
60	16183.5
61	12626.0
62	10665.5
63	8705.0
64	6826.5
65	4948.0
66	3647.5
67	2347.0
68	1884.0
69	1421.0
70	1047.0
71	673.0
72	512.5
73	352.0
74	321.0
75	228.0
76	166.0
77	144.0
78	122.0
79	105.0
80	88.0
81	60.5
82	33.0
83	20.0
84	7.0
85	8.0
86	9.0
87	7.5
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.668872010204772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80977410585533	21.733807109633023
2	8.38841915249878	4.809730301018758
3	3.5315541359548988	3.0373702056240086
4	1.9106877451620985	2.191090496700758
5	1.2396314449455716	1.77694176174849
6	0.9009021302460242	1.5496708719446457
7	0.7192838143167849	1.443473892816186
8	0.5924496366190438	1.3587890243778953
9	0.4907804837266451	1.2663110585759019
>10	5.2283403842820135	34.020824432085654
>50	1.0658393813746718	20.761131961438643
>100	0.12062056628104942	4.730152391256519
>500	8.585093685483943E-4	0.18274777690808158
>1k	4.2925468427419715E-4	0.15628934456112029
>5k	4.2925468427419715E-4	0.9816693713102808
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7977	0.9706753111162219	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1270	0.15453900527987988	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	915	0.11134109435518905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03674864535001868	0.0
2	0.0	0.0	0.0	0.12314446719940034	0.0
3	0.0	0.0	0.0	0.21720639718471305	0.0
4	0.0	0.0	0.0	0.41689026148729796	0.0
5	0.0	0.0	0.0	0.7776840809005608	0.0
6	0.0	0.0	0.0	1.4385512759202677	0.0
7	0.0	0.0	0.0	1.831713107463017	0.0
8	0.0	0.0	0.0	2.6488228873483664	0.0
9	0.0	0.0	0.0	3.0243405017528615	0.0
10	0.0	0.0	0.0	3.585304922493213	0.0
11	0.0	0.0	0.0	4.137508076792501	0.0
12	0.0	0.0	0.0	4.587618140202166	0.0
13	0.0	0.0	0.0	4.811882224242181	0.0
14	0.0	0.0	0.0	4.905944154227494	0.0
15	0.0	0.0	0.0	5.003048190615954	0.0
16	0.0	0.0	0.0	5.19676952636837	0.0
17	0.0	0.0	0.0	5.431985193459715	0.0
18	0.0	0.0	0.0	5.744835415959377	0.0
19	0.0	0.0	0.0	5.921399271598043	0.0
20	0.0	0.0	0.0	6.094190915296807	0.0
21	0.0	0.0	0.0	6.349484484648922	0.0
22	0.0	0.0	0.0	6.614391110234984	0.0
23	0.0	0.0	0.0	6.922252278233485	0.0
24	0.0	0.0	0.0	7.142014044796842	0.0
25	0.0	0.0	0.0	7.345226752527077	0.0
26	0.0	0.0	0.0	7.529700084813927	0.0
27	0.0	0.0	0.0	7.722569630773462	0.0
28	0.0	0.0	0.0	7.9159259137575	0.0
29	0.0	0.0	0.0	8.138973155236256	0.0
30	0.0	0.0	0.0	8.409233888091856	0.0
31	0.0	0.0	0.0	8.674748934958549	0.0
32	0.0	0.0	0.0	8.913980182502048	0.0
33	0.0	0.0	0.0	9.151994587484287	0.0
34	0.0	0.0	0.0	9.387210254575633	0.0
35	0.0	0.0	0.0	9.65248193293007	0.0
36	0.0	0.0	0.0	9.897432340511488	0.0
37	0.0	0.0	0.0	10.174142338941762	0.0
38	0.0	0.0	0.0	10.440387491345207	0.0
39	0.0	0.0	0.0	10.720261280434753	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	30	2.164743E-6	45.000004	2
CGTAAGG	130	0.0	45.000004	2
TGCAGCG	30	2.164743E-6	45.000004	1
CGGACGG	30	2.164743E-6	45.000004	2
CGGGTTC	40	6.8102963E-9	45.0	6
AACGTAC	25	3.8898324E-5	45.0	11
CGGGTCA	50	2.1827873E-11	45.0	6
TTGGCCG	25	3.8898324E-5	45.0	36
TACCCGG	20	7.032112E-4	45.0	16
CGAACGG	25	3.8898324E-5	45.0	2
ACACGCG	20	7.032112E-4	45.0	36
GTTTACG	45	3.8380676E-10	45.0	1
CCGTGAC	20	7.032112E-4	45.0	21
TTGTACG	25	3.8898324E-5	45.0	1
TCGATAG	40	6.8102963E-9	45.0	1
CCGTATC	20	7.032112E-4	45.0	40
AGACGAG	25	3.8898324E-5	45.0	1
TCGTGCG	25	3.8898324E-5	45.0	1
ATATACG	20	7.032112E-4	45.0	1
CGGATCG	20	7.032112E-4	45.0	20
>>END_MODULE