FastQCFastQC Report
Thu 26 May 2016
SRR1547438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547438_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2313827
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426621.843785209525172No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT34770.15027052584311618No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT25790.1114603641499559No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCT24360.10528012682019874No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTA207.0347707E-445.030
TATTCGA207.0347707E-445.020
ATCGCGA207.0347707E-445.024
CGGTCTA2950.043.47457531
CGTTTTT160450.043.4714851
CGACGGT3100.039.91935328
CGTTAGG2400.039.3750042
ATAACGC1200.039.37500411
TCACGAC3200.039.37525
TAGGGAT42200.039.3483435
TAGGGAC26000.039.1153875
GTTAGCG1500.039.01
AGGGCGA21150.038.7234046
GTCTACG1050.038.571431
CAATCGT356.2510353E-638.57142628
TTAGGGA33650.038.2466554
CACGACG3250.038.07692326
CGAGGGA13350.037.921354
TAAGGGA31950.037.8873254
AGGGATT39250.037.777076
Produced by FastQC (version 0.11.5)