##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547438_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2313827
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283965050109625	31.0	31.0	33.0	30.0	34.0
2	31.68837039242778	31.0	31.0	34.0	30.0	34.0
3	31.739092421343514	31.0	31.0	34.0	30.0	34.0
4	35.55283216938864	37.0	35.0	37.0	33.0	37.0
5	35.46021591069687	37.0	35.0	37.0	33.0	37.0
6	35.51538295646131	37.0	35.0	37.0	33.0	37.0
7	35.91619382088635	37.0	35.0	37.0	35.0	37.0
8	35.95640253139064	37.0	35.0	37.0	35.0	37.0
9	37.70556139244637	39.0	37.0	39.0	35.0	39.0
10	37.05700728706165	39.0	37.0	39.0	33.0	39.0
11	36.74585265017652	39.0	37.0	39.0	32.0	39.0
12	36.39940496847863	38.0	35.0	39.0	32.0	39.0
13	36.29176857215341	39.0	35.0	39.0	32.0	39.0
14	37.462290395954405	40.0	36.0	41.0	32.0	41.0
15	37.59201012003058	40.0	36.0	41.0	32.0	41.0
16	37.62429516121992	40.0	36.0	41.0	32.0	41.0
17	37.55465685204641	39.0	36.0	41.0	32.0	41.0
18	37.38703757886826	39.0	36.0	41.0	32.0	41.0
19	37.25698420841316	39.0	36.0	41.0	32.0	41.0
20	37.05540690812234	39.0	35.0	41.0	32.0	41.0
21	36.99566302925845	39.0	35.0	41.0	32.0	41.0
22	36.900082417570545	38.0	35.0	41.0	31.0	41.0
23	36.802298961849786	38.0	35.0	40.0	31.0	41.0
24	36.68747274536947	38.0	35.0	40.0	31.0	41.0
25	36.56120963235367	38.0	35.0	40.0	31.0	41.0
26	36.41841460057299	38.0	35.0	40.0	31.0	41.0
27	36.31235135556807	38.0	35.0	40.0	31.0	41.0
28	36.197896817696396	38.0	35.0	40.0	30.0	41.0
29	36.17177645519739	38.0	35.0	40.0	30.0	41.0
30	36.0919787866595	38.0	35.0	40.0	30.0	41.0
31	35.95508566543653	38.0	35.0	40.0	30.0	41.0
32	35.746535069389374	38.0	35.0	40.0	29.0	41.0
33	35.536743671847546	38.0	34.0	40.0	27.0	41.0
34	35.34686387530269	38.0	34.0	40.0	26.0	41.0
35	35.16650769482766	38.0	34.0	40.0	25.0	41.0
36	35.077915073166665	38.0	34.0	40.0	25.0	41.0
37	35.009277703129925	38.0	34.0	40.0	25.0	41.0
38	34.898952255289615	38.0	34.0	40.0	24.0	41.0
39	34.82855978428811	38.0	34.0	40.0	24.0	41.0
40	34.741838953387614	37.0	34.0	40.0	24.0	41.0
41	34.65479571290334	37.0	34.0	40.0	23.0	41.0
42	34.575948417924074	37.0	34.0	40.0	23.0	41.0
43	34.52811035570075	37.0	33.0	40.0	23.0	41.0
44	34.38629681475754	37.0	33.0	40.0	23.0	41.0
45	34.2717039778687	37.0	33.0	40.0	23.0	41.0
46	34.224168876929866	37.0	33.0	40.0	23.0	41.0
47	34.18887280682609	36.0	33.0	40.0	23.0	41.0
48	34.10307036783649	36.0	33.0	40.0	23.0	41.0
49	34.02522444417841	36.0	33.0	40.0	23.0	41.0
50	33.92629181006186	36.0	33.0	40.0	23.0	41.0
51	33.39674184802926	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	2.0
12	8.0
13	3.0
14	22.0
15	46.0
16	162.0
17	403.0
18	904.0
19	1849.0
20	3267.0
21	5631.0
22	8741.0
23	13227.0
24	20335.0
25	30151.0
26	40888.0
27	46194.0
28	48779.0
29	52781.0
30	60560.0
31	72268.0
32	88378.0
33	112529.0
34	170466.0
35	244178.0
36	210994.0
37	232920.0
38	322161.0
39	525733.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.268122897692866	23.101683920189366	28.449274729701052	18.180918452416712
2	33.77218780833658	24.713602183741482	28.865122586952264	12.649087420969673
3	26.889175379144593	24.46993660286616	35.55036742159202	13.090520596397223
4	23.83224847838667	27.32447153568525	34.79901479237644	14.044265193551636
5	21.725003641153812	31.03537991388293	33.3095343774621	13.930082067501157
6	19.88506487304366	40.04171444105371	29.92125167525489	10.151969010647727
7	85.43240268179082	3.197170747856257	9.77233820851775	1.598088361835176
8	86.18016818024856	2.6345098401911637	9.42974561192345	1.75557636763682
9	81.14132992656754	5.376374292460067	11.02078936757156	2.461506413400829
10	39.55369178421723	33.28662860274342	16.05132968022242	11.10834993281693
11	28.274023943881716	23.667759084840828	30.76379521891654	17.29442175236092
12	27.963110465907782	22.458334179694507	31.516098653875158	18.062456700522553
13	21.253965832363438	28.94693509929653	32.37104589063919	17.42805317770084
14	17.36586183841748	31.31655910316545	32.199598327792	19.117980730625064
15	16.104834112489826	27.325768088971213	38.75566323670698	17.81373456183198
16	18.864331689447827	25.298390934153677	37.88368793345397	17.953589442944523
17	19.229570750103615	25.92324318110213	31.26257926802652	23.584606800767734
18	19.98744936419188	27.40165967464292	33.07183294170221	19.539058019462992
19	23.440213983154315	28.95142981735454	28.240227121560945	19.3681290779302
20	24.59652342201902	27.175497563128097	28.985918134761157	19.242060880091728
21	22.757319367437585	29.034884630527692	29.904310045651638	18.303485956383085
22	22.962995937034187	26.08077440534664	29.2019239122026	21.754305745416577
23	19.70060855889399	29.824572018564915	29.86601850527287	20.60880091726823
24	19.308833374318823	27.056301097705234	35.15863545545972	18.476230072516227
25	19.244610768220788	29.3937273616394	31.701376118439278	19.66028575170054
26	18.98625091677122	32.55053208385934	28.512719403827514	19.95049759554193
27	18.44143922600955	31.785911392684067	30.317521577888062	19.45512780341832
28	17.982545799664365	29.75200825299385	33.76821171159296	18.497234235748827
29	18.140811737437588	27.66766919047967	33.14556360523064	21.045955466852103
30	19.143349956587073	30.40443386649045	31.673024819919554	18.779191357002922
31	23.478678397304552	29.330412342841534	27.8888179626221	19.302091297231815
32	24.042938387355665	28.933364508236785	28.4991055943249	18.524591510082647
33	22.71418736145788	29.7759512703413	27.35252030510492	20.157341063095902
34	19.983213956791065	29.96797081199243	28.91503988846184	21.133775342754664
35	20.799523905633393	28.9213497811202	29.944373542187897	20.334752771058508
36	24.169222677408467	29.571052632716277	27.10837932135808	19.15134536851718
37	20.51652954175053	32.02776179895904	28.610133774046204	18.84557488524423
38	20.296763759779793	31.68119310562112	28.21892042922829	19.8031227053708
39	20.419504137517627	31.971059201919587	27.238985455697424	20.370451204865358
40	22.329413564626915	30.200961437479982	27.777789782900797	19.691835214992302
41	19.55349297938005	29.016127826324094	28.927011397135566	22.50336779716029
42	20.808383686420807	29.80339498156085	27.36436215845005	22.02385917356829
43	21.036966030736092	30.3033459286282	27.5309692556963	21.12871878493941
44	20.53545921972559	31.290930566546244	28.024999276091084	20.148610937637084
45	19.41908362206855	32.934398293390124	26.372585331574054	21.27393275296727
46	20.949880868362243	32.311966279242135	26.684881799719683	20.05327105267593
47	20.364703151964257	30.163188518415595	28.495172716024147	20.976935613596005
48	20.701331603443126	29.080436869307864	30.258528403376744	19.95970312387227
49	19.882947169343257	29.034322790770446	29.566471477772538	21.516258562113762
50	18.98076217452731	31.21382886447431	29.963130346391498	19.842278614606883
51	18.67062662852495	31.0620456931309	28.716840109480962	21.550487568863186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2561.0
1	3009.0
2	3457.0
3	29996.0
4	56535.0
5	36563.0
6	16591.0
7	17498.5
8	18406.0
9	21403.5
10	24401.0
11	27356.0
12	30311.0
13	30217.5
14	30124.0
15	28011.5
16	25899.0
17	23278.0
18	20657.0
19	19158.0
20	17659.0
21	16985.0
22	16311.0
23	16483.0
24	16655.0
25	18558.5
26	23279.0
27	26096.0
28	32302.5
29	38509.0
30	40601.0
31	42693.0
32	48821.0
33	54949.0
34	63425.0
35	71901.0
36	76163.0
37	80425.0
38	89179.0
39	97933.0
40	110981.5
41	124030.0
42	136378.5
43	148727.0
44	153197.5
45	157668.0
46	159181.5
47	160695.0
48	169472.0
49	178249.0
50	173183.5
51	168118.0
52	158053.0
53	147988.0
54	133475.5
55	118963.0
56	107831.5
57	96700.0
58	90693.0
59	84686.0
60	76231.5
61	67777.0
62	58787.0
63	49797.0
64	42120.0
65	34443.0
66	29014.5
67	23586.0
68	18683.0
69	13780.0
70	12010.5
71	10241.0
72	8263.5
73	6286.0
74	5648.5
75	3580.0
76	2149.0
77	1816.0
78	1483.0
79	950.0
80	417.0
81	401.0
82	385.0
83	223.0
84	61.0
85	46.0
86	31.0
87	20.0
88	9.0
89	8.0
90	7.0
91	6.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2313827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.646369123990482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.26757681329678	15.74648543079623
2	7.65534646392338	3.161102177323948
3	3.1798337499319005	1.969560640620506
4	1.8818127846463912	1.5541040549621523
5	1.3077563041571931	1.350020968992749
6	0.9224233077397109	1.1426815260099805
7	0.677448041328231	0.9790789626510912
8	0.5073964545543888	0.8380715594347179
9	0.4175632997127523	0.7759049416650858
>10	4.6322437971334125	22.783716745768068
>50	1.8512127333597892	27.538498377644526
>100	0.694505827638312	19.069409399448983
>500	0.002758499717815488	0.407827314364103
>1k	0.0019097305738722608	0.8145155686601572
>5k	0.0	0.0
>10k+	2.1219228598580678E-4	1.8690223316576822
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42662	1.843785209525172	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT	3477	0.15027052584311618	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2579	0.1114603641499559	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCT	2436	0.10528012682019874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04092786539356659	0.0
2	0.0	0.0	0.0	0.12701900358151236	0.0
3	0.0	0.0	0.0	0.24604259523291933	0.0
4	0.0	0.0	0.0	0.3778588459724949	0.0
5	0.0	0.0	0.0	0.6844072612170227	0.0
6	0.0	0.0	0.0	1.3269358512974392	0.0
7	0.0	0.0	0.0	1.736084849904509	0.0
8	0.0	0.0	0.0	2.4287468337088294	0.0
9	0.0	0.0	0.0	2.811835111268042	0.0
10	0.0	0.0	0.0	3.249854029709222	0.0
11	0.0	0.0	0.0	3.6209707985947093	0.0
12	0.0	0.0	0.0	3.9066014874923667	0.0
13	0.0	0.0	0.0	4.086822394241229	0.0
14	0.0	0.0	0.0	4.190762749332599	0.0
15	4.3218442865434624E-5	0.0	0.0	4.2749090575916	0.0
16	4.3218442865434624E-5	0.0	0.0	4.407416803417023	0.0
17	4.3218442865434624E-5	0.0	0.0	4.551118125944593	0.0
18	4.3218442865434624E-5	0.0	0.0	4.737389614694616	0.0
19	4.3218442865434624E-5	0.0	0.0	4.848201702201591	0.0
20	4.3218442865434624E-5	0.0	0.0	4.966015177452766	0.0
21	4.3218442865434624E-5	0.0	0.0	5.1129146647523775	0.0
22	4.3218442865434624E-5	0.0	0.0	5.263530938138417	0.0
23	4.3218442865434624E-5	0.0	0.0	5.435108156314193	0.0
24	4.3218442865434624E-5	0.0	0.0	5.575265566526797	0.0
25	8.643688573086925E-5	0.0	0.0	5.6873309888768695	0.0
26	8.643688573086925E-5	0.0	0.0	5.807002857171258	4.3218442865434624E-5
27	8.643688573086925E-5	0.0	0.0	5.931860938609498	4.3218442865434624E-5
28	8.643688573086925E-5	0.0	0.0	6.0546445347901985	4.3218442865434624E-5
29	8.643688573086925E-5	0.0	0.0	6.1913444695735675	4.3218442865434624E-5
30	8.643688573086925E-5	0.0	0.0	6.375584691508916	4.3218442865434624E-5
31	8.643688573086925E-5	0.0	0.0	6.516692907464559	4.3218442865434624E-5
32	8.643688573086925E-5	0.0	0.0	6.661950093935285	4.3218442865434624E-5
33	8.643688573086925E-5	0.0	0.0	6.806299693105837	4.3218442865434624E-5
34	8.643688573086925E-5	0.0	0.0	6.955403320991587	4.3218442865434624E-5
35	8.643688573086925E-5	0.0	0.0	7.124646743252629	4.3218442865434624E-5
36	8.643688573086925E-5	0.0	0.0	7.276429914596035	4.3218442865434624E-5
37	8.643688573086925E-5	0.0	0.0	7.433917920397679	4.3218442865434624E-5
38	8.643688573086925E-5	0.0	0.0	7.598191221729196	4.3218442865434624E-5
39	8.643688573086925E-5	0.0	0.0	7.765749124718486	4.3218442865434624E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTA	20	7.0347707E-4	45.0	30
TATTCGA	20	7.0347707E-4	45.0	20
ATCGCGA	20	7.0347707E-4	45.0	24
CGGTCTA	295	0.0	43.474575	31
CGTTTTT	16045	0.0	43.471485	1
CGACGGT	310	0.0	39.919353	28
CGTTAGG	240	0.0	39.375004	2
ATAACGC	120	0.0	39.375004	11
TCACGAC	320	0.0	39.375	25
TAGGGAT	4220	0.0	39.348343	5
TAGGGAC	2600	0.0	39.115387	5
GTTAGCG	150	0.0	39.0	1
AGGGCGA	2115	0.0	38.723404	6
GTCTACG	105	0.0	38.57143	1
CAATCGT	35	6.2510353E-6	38.571426	28
TTAGGGA	3365	0.0	38.246655	4
CACGACG	325	0.0	38.076923	26
CGAGGGA	1335	0.0	37.92135	4
TAAGGGA	3195	0.0	37.887325	4
AGGGATT	3925	0.0	37.77707	6
>>END_MODULE