FastQCFastQC Report
Thu 26 May 2016
SRR1547437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547437_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1524708
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65330.42847548514207306No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22630.1484218617597599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG453.8562575E-1045.01
CGAACGT207.034013E-445.037
ACGTTAG453.8562575E-1045.01
GCGCGAA253.8914077E-545.034
CGACAGT253.8914077E-545.012
TTAACGC351.2122837E-745.036
CACCGTA207.034013E-445.017
CCGTTAG453.8562575E-1045.01
TCACGAC2150.041.86046625
TACGGGA5100.041.470594
CGAGGGA9350.040.187174
TTGGGAC11650.040.1716735
TCGTACA451.9290383E-840.034
ATACCGG900.040.02
CACGACG2200.038.86363626
GCTACGA1800.038.7510
CGTTTTT33600.038.705361
CGGTTAT356.249633E-638.57142622
CGGTCTA2350.038.2978731
ATAGGGA21100.038.281994