##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547437_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1524708
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226808674185484	31.0	31.0	33.0	30.0	34.0
2	31.629836663807104	31.0	31.0	34.0	30.0	34.0
3	31.792427140147492	31.0	31.0	34.0	30.0	34.0
4	35.53762425329965	37.0	35.0	37.0	33.0	37.0
5	35.410968526432605	37.0	35.0	37.0	33.0	37.0
6	35.45163664124541	37.0	35.0	37.0	33.0	37.0
7	35.85616918124651	37.0	35.0	37.0	35.0	37.0
8	35.88028002738885	37.0	35.0	37.0	35.0	37.0
9	37.58251612767822	39.0	37.0	39.0	35.0	39.0
10	36.92812197483059	39.0	37.0	39.0	32.0	39.0
11	36.65241541331193	39.0	35.0	39.0	32.0	39.0
12	36.135511848826134	38.0	35.0	39.0	32.0	39.0
13	35.93243624352991	38.0	35.0	39.0	31.0	39.0
14	37.021138473727426	39.0	35.0	41.0	31.0	41.0
15	37.17851024589626	39.0	35.0	41.0	32.0	41.0
16	37.2487217224544	39.0	35.0	41.0	32.0	41.0
17	37.16513981693544	39.0	35.0	41.0	32.0	41.0
18	37.09399045587745	39.0	35.0	41.0	32.0	41.0
19	37.03779608948074	39.0	35.0	41.0	31.0	41.0
20	36.93161969373808	39.0	35.0	41.0	31.0	41.0
21	36.84020743644029	38.0	35.0	41.0	31.0	41.0
22	36.74079561463572	38.0	35.0	41.0	31.0	41.0
23	36.65189662545222	38.0	35.0	40.0	31.0	41.0
24	36.57741613476154	38.0	35.0	40.0	31.0	41.0
25	36.45754006668818	38.0	35.0	40.0	30.0	41.0
26	36.29793442416515	38.0	35.0	40.0	30.0	41.0
27	36.197146601185274	38.0	35.0	40.0	30.0	41.0
28	36.095805229591505	38.0	35.0	40.0	30.0	41.0
29	36.17597336670366	38.0	35.0	40.0	30.0	41.0
30	36.142871290765186	38.0	35.0	40.0	30.0	41.0
31	36.07327370224331	38.0	35.0	40.0	30.0	41.0
32	36.00252769710659	38.0	35.0	40.0	30.0	41.0
33	35.94762406965793	38.0	35.0	40.0	30.0	41.0
34	35.84337984715762	38.0	35.0	40.0	29.0	41.0
35	35.761962290484476	38.0	35.0	40.0	29.0	41.0
36	35.71732423519782	38.0	35.0	40.0	29.0	41.0
37	35.66947244980678	38.0	34.0	40.0	28.0	41.0
38	35.56537841999911	38.0	34.0	40.0	28.0	41.0
39	35.491336045983886	38.0	34.0	40.0	27.0	41.0
40	35.3806302583839	38.0	34.0	40.0	27.0	41.0
41	35.30900801989627	38.0	34.0	40.0	27.0	41.0
42	35.27358484378648	38.0	34.0	40.0	27.0	41.0
43	35.23886868829966	38.0	34.0	40.0	27.0	41.0
44	35.131251360916316	37.0	34.0	40.0	27.0	41.0
45	34.9738297431377	37.0	34.0	40.0	26.0	41.0
46	34.912459303683065	37.0	34.0	40.0	26.0	41.0
47	34.877767415137846	37.0	34.0	40.0	26.0	41.0
48	34.82969198036608	37.0	34.0	40.0	26.0	41.0
49	34.758623290492345	37.0	34.0	40.0	26.0	41.0
50	34.63457265259971	36.0	33.0	40.0	26.0	41.0
51	34.08669200922406	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	4.0
14	16.0
15	42.0
16	98.0
17	268.0
18	606.0
19	1244.0
20	2060.0
21	3371.0
22	5244.0
23	7504.0
24	10920.0
25	15172.0
26	20236.0
27	24867.0
28	29021.0
29	33900.0
30	40641.0
31	49712.0
32	61465.0
33	77502.0
34	120496.0
35	166659.0
36	121652.0
37	152681.0
38	219049.0
39	360072.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.26944175540497	23.41235174210406	28.01802049966289	13.300186002828083
2	30.713487434971153	26.196163462118648	28.828798694569713	14.261550408340481
3	29.623901756926575	26.11536110520834	30.421431513443885	13.8393056244212
4	26.684125747356212	28.234061866272096	29.65984306503278	15.421969321338905
5	24.040472011690106	32.58341925142388	28.108791978529656	15.267316758356353
6	21.89317561133017	41.61491905335317	25.898467116326536	10.593438218990128
7	88.49261629111935	4.0094234437020075	5.656296156378795	1.8416641087998489
8	88.73036673251534	3.5858669332095063	5.508267812591001	2.175498521684152
9	82.80024765397702	6.474551192752973	7.589453193660687	3.1357479596093154
10	40.31853968104057	35.60452230853383	12.969302974733523	11.10763503569208
11	35.5193912539319	23.071171660409732	24.731686329448	16.67775075621037
12	34.31516067338795	21.342643968550043	26.90784071441876	17.43435464364324
13	24.80396246363238	29.440784727305164	27.043538828418296	18.711713980644163
14	19.670717278324766	32.1435317450948	28.314667464196425	19.87108351238401
15	18.39506318586903	26.449064345435325	37.122124367419865	18.033748101275783
16	21.567014798899198	23.892115736259008	36.14403544809892	18.396834016742876
17	20.802999656327636	24.109140897798138	28.702413839240037	26.38544560663419
18	22.9561332399384	25.6960677060788	30.053033105355254	21.29476594862754
19	26.362555977931514	26.67966587700727	26.582532524260383	20.375245620800836
20	29.910317254189	24.82927878649551	25.859508837101924	19.400895122213566
21	26.005307245715247	27.319919617395595	26.806641009294896	19.86813212759427
22	25.01042822625709	24.80665150310748	26.497598228644435	23.685322041990993
23	22.396288338488418	29.005947368282975	26.811494397615803	21.7862698956128
24	22.6931976483366	25.06532398334632	32.022393796058005	20.21908457225908
25	21.581902895505237	26.689831757949715	29.83056427853727	21.897701068007777
26	21.39819558892588	30.135475120482084	26.991200938146847	21.47512835244519
27	21.211274552242134	30.242512008856774	27.442172534019626	21.10404090488146
28	18.631108382719837	29.352767874242154	31.69387187579523	20.322251867242777
29	20.39321627485394	26.178061635408223	31.06870299099893	22.360019098738906
30	21.007038724791894	27.72714513205151	29.940224620058398	21.325591523098193
31	26.037575719416438	26.124608777549536	26.129003061569822	21.7088124414642
32	26.061711488363677	27.65480341153847	26.414631522888314	19.86885357720954
33	25.09896976994939	27.483098403104066	25.940835884641515	21.47709594230502
34	21.196583214622077	27.885470529439075	28.87798844106544	22.039957814873407
35	21.919803660766522	26.634608069217187	28.634466402747282	22.81112186726901
36	24.954876605881257	27.771153558583023	26.370032819398865	20.90393701613686
37	22.04494237585164	30.487214601090834	27.048392216739202	20.419450806318324
38	21.75439493988357	30.51076009308012	25.801661695222954	21.933183271813355
39	21.796960467184537	28.816599637438777	26.879573006765884	22.5068668886108
40	23.65856281989732	26.43043782809561	27.776793982847863	22.13420536915921
41	19.120447980859286	26.705900408471656	28.639582136382835	25.534069474286227
42	22.198020866946326	26.81851213478253	25.929686208769155	25.05378078950199
43	21.698318628878447	26.968048964129526	26.888230402149134	24.445402004842894
44	21.73819511670431	28.06314389378163	27.314672711102716	22.88398827841134
45	21.02599317377491	29.763928568617732	25.45831726468281	23.75176099292455
46	21.83670578235308	29.893264808737147	26.118640421641388	22.15138898726838
47	22.07852257612605	27.873599403951445	27.138704591305352	22.90917342861715
48	22.013657697080358	25.671669591816926	29.24054966590324	23.074123045199475
49	21.430660821613056	25.717973539851563	29.40182644808055	23.44953919045483
50	19.91686276978936	28.953937409654834	28.523297575666945	22.605902244888856
51	19.05256613069519	29.551560036413534	27.081054208412365	24.31481962447892
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1474.0
1	1837.5
2	2201.0
3	6664.0
4	11127.0
5	7891.0
6	4655.0
7	4752.5
8	4850.0
9	5216.5
10	5583.0
11	5722.0
12	5861.0
13	5884.5
14	5908.0
15	5794.5
16	5681.0
17	5503.5
18	5326.0
19	5364.0
20	5402.0
21	5445.0
22	5488.0
23	6409.5
24	7331.0
25	8624.5
26	12050.0
27	14182.0
28	18768.0
29	23354.0
30	24967.5
31	26581.0
32	30475.5
33	34370.0
34	39541.0
35	44712.0
36	45596.5
37	46481.0
38	53336.5
39	60192.0
40	73372.5
41	86553.0
42	96622.0
43	106691.0
44	113379.5
45	120068.0
46	122709.5
47	125351.0
48	123771.0
49	122191.0
50	119005.5
51	115820.0
52	105999.0
53	96178.0
54	92527.5
55	88877.0
56	82211.5
57	75546.0
58	72284.0
59	69022.0
60	64337.0
61	59652.0
62	52397.5
63	45143.0
64	37193.5
65	29244.0
66	24471.0
67	19698.0
68	16075.0
69	12452.0
70	10525.0
71	8598.0
72	7021.5
73	5445.0
74	4497.5
75	2834.5
76	2119.0
77	1451.5
78	784.0
79	702.0
80	620.0
81	390.0
82	160.0
83	172.5
84	185.0
85	127.5
86	70.0
87	37.5
88	5.0
89	5.5
90	6.0
91	3.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1524708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.436038576864647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29707632696247	15.023489573696935
2	7.3504061179944085	2.857255537299225
3	2.5729374964507365	1.5002313731063417
4	1.2999457420629015	1.0106318236226197
5	0.8132632720556423	0.7903308164410316
6	0.6082174392558489	0.7092802567499106
7	0.48071702529336086	0.6540264253710165
8	0.3679656858135903	0.5721436211548316
9	0.3437939686495466	0.6013793553449384
>10	5.996708985443424	30.11124255272094
>50	2.357480991674833	32.18335703006061
>100	0.5056862291158726	12.7017593203964
>500	0.004435844115049645	0.5615264727436766
>1k	0.0010236563342422257	0.29008111394600705
>5k	3.4121877808074194E-4	0.4332647273455108
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6533	0.42847548514207306	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2263	0.1484218617597599	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05063264572626365	0.0
2	0.0	0.0	0.0	0.1614735411632916	0.0
3	0.0	0.0	0.0	0.24227589807359837	0.0
4	0.0	0.0	0.0	0.425064996051703	0.0
5	0.0	0.0	0.0	0.7401417189389706	0.0
6	0.0	0.0	0.0	1.162714434501557	0.0
7	0.0	0.0	0.0	1.373574481146554	0.0
8	0.0	0.0	0.0	1.9367642853582456	0.0
9	0.0	0.0	0.0	2.14132804445179	0.0
10	0.0	0.0	0.0	2.516678603378483	0.0
11	0.0	0.0	0.0	2.9106556796448895	0.0
12	0.0	0.0	0.0	3.253541005884405	0.0
13	0.0	0.0	0.0	3.3993394144977267	0.0
14	0.0	0.0	0.0	3.463810775571454	0.0
15	0.0	0.0	0.0	3.5553692903821585	0.0
16	0.0	0.0	0.0	3.7335017590253345	0.0
17	0.0	0.0	0.0	3.9391804857061157	0.0
18	0.0	0.0	0.0	4.1906384697922485	0.0
19	0.0	0.0	0.0	4.326270997463121	0.0
20	0.0	0.0	0.0	4.481317078417638	0.0
21	0.0	0.0	0.0	4.675387024925429	0.0
22	0.0	0.0	0.0	4.887755557129627	0.0
23	0.0	0.0	0.0	5.106092445241974	0.0
24	0.0	0.0	0.0	5.271304407138941	0.0
25	0.0	0.0	0.0	5.42444848456229	0.0
26	0.0	0.0	0.0	5.570312479504272	0.0
27	0.0	0.0	0.0	5.710601636510073	0.0
28	0.0	0.0	0.0	5.8590890845984935	0.0
29	0.0	0.0	0.0	6.026858913313237	0.0
30	0.0	0.0	0.0	6.22447052156872	0.0
31	0.0	0.0	0.0	6.406406997274233	0.0
32	0.0	0.0	0.0	6.587425264378491	0.0
33	0.0	0.0	0.0	6.759130272812893	0.0
34	0.0	0.0	0.0	6.936541291840799	0.0
35	0.0	0.0	0.0	7.129693029747335	0.0
36	6.558632866096328E-5	0.0	0.0	7.330715127093188	0.0
37	6.558632866096328E-5	0.0	0.0	7.537574407689866	0.0
38	6.558632866096328E-5	0.0	0.0	7.7395147136369715	0.0
39	6.558632866096328E-5	0.0	0.0	7.931682656613594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	45	3.8562575E-10	45.0	1
CGAACGT	20	7.034013E-4	45.0	37
ACGTTAG	45	3.8562575E-10	45.0	1
GCGCGAA	25	3.8914077E-5	45.0	34
CGACAGT	25	3.8914077E-5	45.0	12
TTAACGC	35	1.2122837E-7	45.0	36
CACCGTA	20	7.034013E-4	45.0	17
CCGTTAG	45	3.8562575E-10	45.0	1
TCACGAC	215	0.0	41.860466	25
TACGGGA	510	0.0	41.47059	4
CGAGGGA	935	0.0	40.18717	4
TTGGGAC	1165	0.0	40.171673	5
TCGTACA	45	1.9290383E-8	40.0	34
ATACCGG	90	0.0	40.0	2
CACGACG	220	0.0	38.863636	26
GCTACGA	180	0.0	38.75	10
CGTTTTT	3360	0.0	38.70536	1
CGGTTAT	35	6.249633E-6	38.571426	22
CGGTCTA	235	0.0	38.29787	31
ATAGGGA	2110	0.0	38.28199	4
>>END_MODULE