FastQCFastQC Report
Thu 26 May 2016
SRR1547436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547436_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2289014
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT595562.6018189491195773No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT64720.2827418268302422No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT46180.2017462540639769No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC40830.17837374520208266No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG32020.13988555771174838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG406.8193913E-945.01
TACCGCG253.8920225E-545.01
CGTTTTT251600.044.195151
GGCGTAG1200.039.3750041
CAGCGCG403.4602272E-739.3751
CTATACG403.4602272E-739.3751
CGCGCCT403.4602272E-739.37517
GTTTTTT283650.039.32842
CTTAACG750.039.01
CGCCGAC750.039.013
GGCGATA9550.038.6387448
ACGGGTA2100.038.571435
GCGATAT2100.038.571439
GACCGAT10800.038.5416689
GGGCGAT39600.038.2386367
TGGGCGA11450.038.1222726
AGGGCGA20300.037.9064036
TCGCAAG950.037.8947371
CTAAGCG950.037.8947371
GCGATAA2750.037.6363649
Produced by FastQC (version 0.11.5)