##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547436_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2289014
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226365587978055	31.0	31.0	33.0	30.0	34.0
2	31.62496428593272	31.0	31.0	34.0	30.0	34.0
3	31.570137185705285	31.0	31.0	34.0	30.0	34.0
4	35.44072775439556	37.0	35.0	37.0	33.0	37.0
5	35.35462823731091	37.0	35.0	37.0	33.0	37.0
6	35.41940722075094	37.0	35.0	37.0	33.0	37.0
7	35.907819698787335	37.0	35.0	37.0	35.0	37.0
8	35.959608372862725	37.0	35.0	37.0	35.0	37.0
9	37.68560262191494	39.0	37.0	39.0	35.0	39.0
10	36.98128014944426	39.0	37.0	39.0	32.0	39.0
11	36.6664480863813	39.0	35.0	39.0	32.0	39.0
12	36.42722368670528	39.0	35.0	39.0	32.0	39.0
13	36.372469106785715	39.0	35.0	39.0	32.0	39.0
14	37.53477348762393	40.0	36.0	41.0	32.0	41.0
15	37.643222802481766	40.0	36.0	41.0	32.0	41.0
16	37.64624550133813	40.0	36.0	41.0	32.0	41.0
17	37.57359981197144	40.0	36.0	41.0	32.0	41.0
18	37.352656209180026	39.0	36.0	41.0	32.0	41.0
19	37.172907199344344	38.0	36.0	41.0	32.0	41.0
20	36.9281896047818	38.0	35.0	41.0	32.0	41.0
21	36.87626069565324	38.0	35.0	41.0	31.0	41.0
22	36.78914370991178	38.0	35.0	41.0	31.0	41.0
23	36.70459770014513	38.0	35.0	40.0	31.0	41.0
24	36.59027729843505	38.0	35.0	40.0	31.0	41.0
25	36.47644531662978	38.0	35.0	40.0	31.0	41.0
26	36.31876694506892	38.0	35.0	40.0	31.0	41.0
27	36.21783309320083	38.0	35.0	40.0	30.0	41.0
28	36.092055793455174	38.0	35.0	40.0	30.0	41.0
29	36.038281985169164	38.0	35.0	40.0	30.0	41.0
30	35.89398928971164	38.0	35.0	40.0	30.0	41.0
31	35.6564730490945	38.0	35.0	40.0	29.0	41.0
32	35.349672828562866	38.0	34.0	40.0	27.0	41.0
33	35.009964989292335	38.0	34.0	40.0	24.0	41.0
34	34.692881738600114	38.0	34.0	40.0	23.0	41.0
35	34.44047000149409	38.0	33.0	40.0	21.0	41.0
36	34.35181829381559	38.0	33.0	40.0	21.0	41.0
37	34.28700348709095	38.0	33.0	40.0	21.0	41.0
38	34.1831522218737	38.0	33.0	40.0	20.0	41.0
39	34.093417515139706	38.0	33.0	40.0	19.0	41.0
40	34.01780417245155	37.0	33.0	40.0	18.0	41.0
41	33.91132382763932	37.0	33.0	40.0	18.0	41.0
42	33.85152297015222	37.0	33.0	40.0	18.0	41.0
43	33.80657610656816	37.0	33.0	40.0	18.0	41.0
44	33.67350177849502	37.0	33.0	40.0	18.0	41.0
45	33.559351318952174	37.0	33.0	40.0	18.0	41.0
46	33.526827708349536	37.0	33.0	40.0	18.0	41.0
47	33.471714895583865	37.0	33.0	40.0	18.0	41.0
48	33.374088581371716	36.0	33.0	40.0	18.0	41.0
49	33.27842686851194	36.0	32.0	40.0	18.0	41.0
50	33.18713603324401	36.0	32.0	40.0	18.0	41.0
51	32.70196294124894	35.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	2.0
12	1.0
13	8.0
14	26.0
15	48.0
16	206.0
17	537.0
18	1311.0
19	2544.0
20	4513.0
21	7255.0
22	11167.0
23	17350.0
24	26551.0
25	41149.0
26	55191.0
27	58667.0
28	57438.0
29	57788.0
30	62846.0
31	73178.0
32	86805.0
33	107343.0
34	153865.0
35	215917.0
36	199780.0
37	221134.0
38	302961.0
39	523132.0
40	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.82786474875208	23.09396098057941	25.81395308198202	21.264221188686484
2	36.986449187291996	23.65258578584491	27.05662787558311	12.304337151279983
3	25.842568022738178	23.814052688188013	37.982467560268304	12.360911728805503
4	22.96892897990139	26.03981452275958	37.79011399668154	13.20114250065749
5	20.86994662330593	30.313881872282128	36.045825844664996	12.770345659746948
6	18.984593366401427	38.56852775911375	32.8818434487513	9.565035425733527
7	81.53938770142952	2.8218700278810003	13.933379175487786	1.7053630952016896
8	82.09622134246447	2.275958119959074	13.64644340314214	1.9813771344343023
9	76.76527972305979	5.319888825494296	15.287411959909376	2.6274194915365303
10	34.660556903540126	32.910633137237255	21.698600358058098	10.730209601164518
11	24.255946009941397	25.58210653145852	33.95671673480372	16.20523072379636
12	23.469537538870448	23.673948695814005	35.215293571817384	17.64122019349816
13	19.73360582329335	27.655706780299287	36.28772038965249	16.322967006754872
14	16.519296081194785	30.94843456614944	35.59855903022	16.933710322435775
15	15.739484336924109	28.184711845362237	40.279744903264024	15.79605891444963
16	18.045368005612897	27.116173164515377	39.28010051489419	15.558358314977541
17	18.273894349270034	27.242209964639798	34.19380571722147	20.290089968868692
18	19.096082418019286	27.899217741787513	35.894450623718335	17.110249216474866
19	20.66671501353858	29.942499259506494	32.343795188670754	17.04699053828417
20	21.967362366503657	28.72808991120194	32.29342415555344	17.011123566740963
21	19.827095858740925	29.641452607978806	33.709230262462356	16.822221270817916
22	19.845007501046304	27.082577913459684	33.637277884713676	19.435136700780337
23	17.16730435025736	30.574736545953847	33.30617462365892	18.95178448012987
24	17.15489726144095	29.405892668196877	36.69475153930907	16.74445853105311
25	18.33614822801433	29.809734671784444	34.347015789331124	17.507101310870095
26	17.591853959827244	32.4447993765001	32.51963509179062	17.44371157188204
27	17.520600572997804	31.297187347914868	33.516614577280876	17.665597501806456
28	16.691029412664143	30.56106253609633	35.95478227743474	16.79312577380479
29	17.52890109016371	28.570074276522554	35.11590580048877	18.785118832824963
30	18.50726994242936	30.070589345456167	33.961565984305906	17.460574727808567
31	20.165407463650286	29.361725179487763	32.61146502380501	17.86140233305694
32	20.58790378739492	29.89986081343321	31.763632725706355	17.74860267346552
33	19.49857012670084	31.1477343519961	31.25865547349208	18.09504004781098
34	17.698886944334983	31.475997962441472	31.5398682576865	19.28524683553705
35	17.79683304689268	31.75581276479742	31.45677571216253	18.99057847614737
36	19.734916431266914	31.73213444740836	29.785794232800672	18.747154888524054
37	17.952970143476623	32.99757013281701	30.56193627474537	18.487523448960992
38	18.059828380254555	32.66026332735405	29.701653200897855	19.578255091493542
39	17.875775333833694	32.11998703371845	29.599775274419464	20.40446235802839
40	19.87742320492579	30.87530264122456	30.072424196619156	19.174849957230496
41	17.44165828605679	30.715714276976897	30.561018849163872	21.281608587802435
42	19.211896475949906	31.58499685890956	28.93748137844504	20.265625286695492
43	19.080267748471613	31.53322784395377	29.67447992891262	19.71202447866199
44	19.350558799552996	32.603208193571554	29.413013638186573	18.633219368688877
45	18.05764403363195	33.71805502281768	28.212627795199158	20.01167314835121
46	19.28673219124042	32.67808759579452	28.36937650883743	19.66580370412763
47	19.21687678624945	31.45266914051203	29.47203468392941	19.85841938930911
48	19.14086152378273	30.58037216024017	30.19706301490511	20.08170330107199
49	18.802768353535626	30.515191257021584	30.280461368956242	20.401579020486547
50	17.672936906458414	31.667652535109003	31.07766051234287	19.581750046089713
51	17.348124563676762	32.31854414171342	29.808904620067857	20.524426674541964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2172.0
1	2495.5
2	2819.0
3	40346.5
4	77874.0
5	50618.5
6	23363.0
7	23753.0
8	24143.0
9	27330.0
10	30517.0
11	33925.5
12	37334.0
13	38300.0
14	39266.0
15	37481.5
16	35697.0
17	32959.0
18	30221.0
19	28164.0
20	26107.0
21	24598.5
22	23090.0
23	23028.5
24	22967.0
25	24527.0
26	28902.5
27	31718.0
28	36596.5
29	41475.0
30	46121.5
31	50768.0
32	56866.5
33	62965.0
34	70332.0
35	77699.0
36	85804.5
37	93910.0
38	99568.0
39	105226.0
40	115412.5
41	125599.0
42	136866.5
43	148134.0
44	155161.0
45	162188.0
46	175066.5
47	187945.0
48	186276.5
49	184608.0
50	172249.0
51	159890.0
52	141185.0
53	122480.0
54	109557.0
55	96634.0
56	86013.5
57	75393.0
58	65585.0
59	55777.0
60	48829.5
61	41882.0
62	34510.5
63	27139.0
64	21856.5
65	16574.0
66	12509.5
67	8445.0
68	6558.5
69	4672.0
70	3646.5
71	2621.0
72	2085.5
73	1550.0
74	1197.0
75	698.0
76	552.0
77	476.5
78	401.0
79	265.0
80	129.0
81	93.0
82	57.0
83	46.0
84	35.0
85	28.5
86	22.0
87	17.0
88	12.0
89	10.5
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2289014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.829362450634147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25350659865492	19.92174241765867
2	8.051956715362914	4.320577303065785
3	3.642228631866288	2.931560162772539
4	2.336506755514366	2.5074794644820066
5	1.5965893604721755	2.1417737318467087
6	1.1439249453583442	1.8414466185204525
7	0.8875004150248167	1.6667749216852328
8	0.6950346343712241	1.4917868897031643
9	0.5540859181066675	1.3379194733108555
>10	5.6849547790005905	34.19477597804186
>50	1.0114299176635522	18.42043719900924
>100	0.1389726485864465	5.045598819694027
>500	0.0011580380062331517	0.2072774256313113
>1k	0.0018197740097949527	1.0402042285000883
>5k	1.6543400089045026E-4	0.2872589932946139
>10k+	1.6543400089045026E-4	2.643386372783378
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59556	2.6018189491195773	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	6472	0.2827418268302422	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	4618	0.2017462540639769	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	4083	0.17837374520208266	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	3202	0.13988555771174838	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06810792769288436	0.0
2	0.0	0.0	0.0	0.1998240290360828	0.0
3	0.0	0.0	0.0	0.4280445641660558	0.0
4	0.0	0.0	0.0	0.6280433409319471	0.0
5	0.0	0.0	0.0	1.1135799082050175	0.0
6	0.0	0.0	0.0	2.1038752930300992	0.0
7	0.0	0.0	0.0	2.755946446810723	0.0
8	0.0	0.0	0.0	3.6859538648518533	0.0
9	0.0	0.0	0.0	4.236933456938227	0.0
10	0.0	0.0	0.0	4.7912332558909645	0.0
11	0.0	0.0	0.0	5.215083874541615	0.0
12	0.0	0.0	0.0	5.563093978455353	0.0
13	0.0	0.0	0.0	5.80979408601258	0.0
14	0.0	0.0	0.0	5.964576887690508	0.0
15	0.0	0.0	0.0	6.08738085481347	0.0
16	0.0	0.0	0.0	6.256886152727768	0.0
17	0.0	0.0	0.0	6.45076875895036	0.0
18	0.0	0.0	0.0	6.710924441702847	0.0
19	0.0	0.0	0.0	6.8643529484747585	0.0
20	0.0	0.0	0.0	7.027523641183496	0.0
21	0.0	0.0	0.0	7.206203194912744	0.0
22	0.0	0.0	0.0	7.395760794822574	0.0
23	0.0	0.0	0.0	7.608603529729394	0.0
24	0.0	0.0	0.0	7.7852297976333915	0.0
25	0.0	0.0	0.0	7.9417163896769525	0.0
26	0.0	0.0	0.0	8.09916409423446	0.0
27	0.0	0.0	0.0	8.262946403997528	0.0
28	0.0	0.0	0.0	8.43004892062696	0.0
29	0.0	0.0	0.0	8.61161181189805	0.0
30	0.0	0.0	0.0	8.85140938412784	0.0
31	0.0	0.0	0.0	9.046602598323995	0.0
32	0.0	0.0	0.0	9.24052889147904	0.0
33	0.0	0.0	0.0	9.431003916970363	0.0
34	0.0	0.0	0.0	9.623007985097514	0.0
35	0.0	0.0	0.0	9.835195416017552	0.0
36	0.0	0.0	0.0	10.040349250812795	0.0
37	0.0	0.0	0.0	10.247512684500837	0.0
38	0.0	0.0	0.0	10.473286751413491	0.0
39	4.368693245213878E-5	0.0	0.0	10.742835124643188	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAG	40	6.8193913E-9	45.0	1
TACCGCG	25	3.8920225E-5	45.0	1
CGTTTTT	25160	0.0	44.19515	1
GGCGTAG	120	0.0	39.375004	1
CAGCGCG	40	3.4602272E-7	39.375	1
CTATACG	40	3.4602272E-7	39.375	1
CGCGCCT	40	3.4602272E-7	39.375	17
GTTTTTT	28365	0.0	39.3284	2
CTTAACG	75	0.0	39.0	1
CGCCGAC	75	0.0	39.0	13
GGCGATA	955	0.0	38.638744	8
ACGGGTA	210	0.0	38.57143	5
GCGATAT	210	0.0	38.57143	9
GACCGAT	1080	0.0	38.541668	9
GGGCGAT	3960	0.0	38.238636	7
TGGGCGA	1145	0.0	38.122272	6
AGGGCGA	2030	0.0	37.906403	6
TCGCAAG	95	0.0	37.894737	1
CTAAGCG	95	0.0	37.894737	1
GCGATAA	275	0.0	37.636364	9
>>END_MODULE