##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547435_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2368005
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292907743015746	31.0	31.0	33.0	30.0	34.0
2	31.693583417264744	31.0	31.0	34.0	30.0	34.0
3	31.661379093371846	31.0	31.0	34.0	30.0	34.0
4	35.53101408147364	37.0	35.0	37.0	33.0	37.0
5	35.45260335176657	37.0	35.0	37.0	33.0	37.0
6	35.50839926435966	37.0	35.0	37.0	33.0	37.0
7	35.927375575642785	37.0	35.0	37.0	35.0	37.0
8	35.96702287368481	37.0	35.0	37.0	35.0	37.0
9	37.69739886528956	39.0	37.0	39.0	35.0	39.0
10	37.05917850680214	39.0	37.0	39.0	33.0	39.0
11	36.80482135806301	39.0	37.0	39.0	32.0	39.0
12	36.55270449175572	39.0	35.0	39.0	32.0	39.0
13	36.49983889392126	39.0	35.0	39.0	32.0	39.0
14	37.611057409084864	40.0	36.0	41.0	32.0	41.0
15	37.679446200493665	40.0	36.0	41.0	32.0	41.0
16	37.68233597479735	40.0	36.0	41.0	32.0	41.0
17	37.58261574616608	40.0	36.0	41.0	32.0	41.0
18	37.34922392478057	39.0	36.0	41.0	32.0	41.0
19	37.17740165244584	38.0	36.0	41.0	32.0	41.0
20	36.934122605315444	38.0	35.0	41.0	32.0	41.0
21	36.87825828070464	38.0	35.0	41.0	31.0	41.0
22	36.81582724698639	38.0	35.0	40.0	31.0	41.0
23	36.7328050405299	38.0	35.0	40.0	31.0	41.0
24	36.630000358951946	38.0	35.0	40.0	31.0	41.0
25	36.528650488491365	38.0	35.0	40.0	31.0	41.0
26	36.40457178088729	38.0	35.0	40.0	31.0	41.0
27	36.31373117877707	38.0	35.0	40.0	30.0	41.0
28	36.181944294881134	38.0	35.0	40.0	30.0	41.0
29	36.15859045905731	38.0	35.0	40.0	30.0	41.0
30	36.014273618510096	38.0	35.0	40.0	30.0	41.0
31	35.81591381774954	38.0	35.0	40.0	29.0	41.0
32	35.555488691958	38.0	34.0	40.0	27.0	41.0
33	35.2552410151161	38.0	34.0	40.0	25.0	41.0
34	34.87807120339695	38.0	34.0	40.0	23.0	41.0
35	34.64631029072996	38.0	34.0	40.0	22.0	41.0
36	34.566738668203826	38.0	34.0	40.0	22.0	41.0
37	34.51657576736535	38.0	34.0	40.0	21.0	41.0
38	34.39434038357182	38.0	33.0	40.0	21.0	41.0
39	34.30382494969394	38.0	33.0	40.0	20.0	41.0
40	34.224670978312965	38.0	33.0	40.0	20.0	41.0
41	34.09674557275006	38.0	33.0	40.0	18.0	41.0
42	34.04042516802118	38.0	33.0	40.0	18.0	41.0
43	33.99843412492795	38.0	33.0	40.0	18.0	41.0
44	33.860806037149416	37.0	33.0	40.0	18.0	41.0
45	33.75078937755621	37.0	33.0	40.0	18.0	41.0
46	33.731538573609434	37.0	33.0	40.0	18.0	41.0
47	33.69021011357662	37.0	33.0	40.0	18.0	41.0
48	33.60859584333648	37.0	33.0	40.0	18.0	41.0
49	33.54385907124352	37.0	33.0	40.0	18.0	41.0
50	33.46173720072382	36.0	32.0	40.0	18.0	41.0
51	32.932001410469994	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	2.0
12	4.0
13	11.0
14	26.0
15	56.0
16	171.0
17	477.0
18	1115.0
19	2275.0
20	4058.0
21	6529.0
22	10152.0
23	15480.0
24	24238.0
25	38260.0
26	54255.0
27	62241.0
28	61632.0
29	60999.0
30	64703.0
31	74778.0
32	88554.0
33	110915.0
34	159613.0
35	205743.0
36	194665.0
37	232878.0
38	335166.0
39	558761.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.67713328308006	21.662961015707317	26.702857468628654	21.95704823258397
2	37.881803459029854	22.85856659931039	27.041539185939218	12.218090755720533
3	26.28330598964107	23.754130586717512	37.490545839219095	12.47201758442233
4	23.58791472146385	25.805730984520725	36.98813980544804	13.618214488567382
5	21.03373937132734	29.444067896816094	35.84692599888936	13.675266732967204
6	19.78695146336262	37.52310489209271	32.572186291836374	10.117757352708292
7	81.38707477391306	2.6694622688718987	14.170873794607697	1.7725891626073427
8	81.98145696482905	1.9921410638913346	13.919522973980206	2.1068789972994146
9	76.37935730710028	4.742050798034633	15.937466348255178	2.94112554660991
10	32.725437657437375	32.83329215943379	21.87714975263988	12.564120430488956
11	25.2518470189041	21.7197176526232	35.1818513896719	17.846583938800805
12	25.174862384158818	20.390328567718395	35.350769951921556	19.084039096201234
13	22.60776476401021	21.944379340415242	36.499922930905974	18.94793296466857
14	18.160561316382356	25.189642758355664	36.8369576922346	19.81283823302738
15	18.184716670784056	24.773765258097004	39.498987544367516	17.54253052675142
16	22.479808953106094	23.43753497142109	37.33112894609597	16.75152712937684
17	21.573391948074434	23.824527397535057	34.98810179877154	19.61397885561897
18	21.385554506852813	24.40412921425419	36.35114790720459	17.859168371688405
19	22.91908167423633	26.31565389431188	32.82729555047392	17.93796888097787
20	23.869248586890652	25.7010437055665	32.651958082858776	17.77774962468407
21	22.91713911077046	25.724861222843703	34.56842363086226	16.789576035523574
22	21.46942257300977	22.926091794569693	35.32277170022867	20.281713932191867
23	20.278926775914748	25.397708197406676	34.966775830287524	19.356589196391056
24	20.486316540716764	24.97680536992109	36.41630824259239	18.120569846769747
25	21.651221175630965	25.507125196103893	33.507741748856105	19.333911879409037
26	19.807179461192014	26.830897738813896	33.28164425328494	20.08027854670915
27	18.9092506139134	26.118019176479777	34.730163154216314	20.24256705539051
28	18.757477285732083	26.657122767899562	35.24853199211995	19.336867954248408
29	20.122930483677187	25.343612027846223	34.63877821203925	19.894679276437337
30	20.697802580653335	24.635632103817347	36.035354655078855	18.631210660450463
31	22.23850878693246	25.424777397007187	33.3287725321526	19.007941283907762
32	22.980568030895203	25.688036976273278	33.02053838568753	18.31085660714399
33	22.692646341540666	25.874776446840276	32.41293831727551	19.01963889434355
34	19.670228736848106	27.276716054231304	34.10833169693476	18.944723511985828
35	19.645144330354032	28.19225466162445	32.58785348848503	19.574747519536487
36	20.74214370324387	29.122953709979498	30.567038498651815	19.567864088124814
37	20.758486574141525	29.214887637483873	30.242714859132473	19.783910929242126
38	20.16389323502273	28.99440668410751	30.571557070191997	20.270143010677767
39	19.955109892082152	28.334821928163155	29.651373202336988	22.0586949774177
40	21.307007375406727	26.59082223221657	31.123794079826688	20.978376312550015
41	19.036150683803456	27.594325180901226	31.0253567876757	22.34416734761962
42	20.16874964368741	28.530767460372765	29.863492686881997	21.436990209057836
43	20.186232714880248	27.22730737477328	30.603060382051556	21.983399528294914
44	20.845395174418975	27.851081395520698	30.03747880599914	21.26604462406118
45	20.341891169993307	27.717973568467976	30.11019824704762	21.8299370144911
46	20.670311084647203	28.39656166266541	29.825697158578635	21.107430094108754
47	20.3032510488787	27.885034026532885	30.827975447686978	20.98373947690144
48	19.74670661590664	27.232206013078518	31.728480303039902	21.29260706797494
49	20.796957776693883	26.697916600682852	31.10191912601536	21.403206496607904
50	19.273016737718038	26.938329944404675	32.473284473639204	21.315368844238083
51	18.704901383231874	27.26147115398827	32.266232545961685	21.767394916818166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1821.0
1	2580.5
2	3340.0
3	42691.5
4	82043.0
5	54548.0
6	27053.0
7	26451.5
8	25850.0
9	26830.0
10	27810.0
11	27948.0
12	28086.0
13	27511.0
14	26936.0
15	25745.5
16	24555.0
17	22580.0
18	20605.0
19	19277.0
20	17949.0
21	17400.5
22	16852.0
23	17150.5
24	17449.0
25	18598.5
26	22617.5
27	25487.0
28	28763.0
29	32039.0
30	37029.0
31	42019.0
32	46317.5
33	50616.0
34	57375.5
35	64135.0
36	72612.0
37	81089.0
38	86476.5
39	91864.0
40	101321.0
41	110778.0
42	124146.0
43	137514.0
44	156076.0
45	174638.0
46	180901.0
47	187164.0
48	183650.0
49	180136.0
50	166644.5
51	153153.0
52	144099.0
53	135045.0
54	127659.5
55	120274.0
56	114414.5
57	108555.0
58	103228.0
59	97901.0
60	88402.0
61	78903.0
62	69046.0
63	59189.0
64	48849.0
65	38509.0
66	30522.0
67	22535.0
68	18295.5
69	14056.0
70	11337.0
71	8618.0
72	7223.0
73	5828.0
74	4655.5
75	2878.5
76	2274.0
77	1687.5
78	1101.0
79	831.5
80	562.0
81	423.0
82	284.0
83	197.5
84	111.0
85	67.5
86	24.0
87	18.0
88	12.0
89	8.0
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2368005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.735018943677392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34515586322354	19.341491124865748
2	7.426128688374499	3.9707538232375676
3	3.4688053613187413	2.782157311403587
4	2.2100437130983157	2.363422421441544
5	1.592735239814448	2.129090340435091
6	1.2503617757193057	2.005706745618345
7	1.0175429029904026	1.9042820151217028
8	0.854561175405588	1.8277367370399693
9	0.7380541005932747	1.7758701324737982
>10	8.617938995994958	49.2596459711924
>50	0.438146545226099	7.313209274402073
>100	0.03652176905770667	1.6693852510139278
>500	0.0022421667426046027	0.41117556311415937
>1k	0.001601547673289002	0.8963824235962642
>5k	0.0	0.0
>10k+	1.601547673289002E-4	2.3496908650438115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54877	2.3174359851436126	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	4785	0.20206883009115267	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	3213	0.13568383512703733	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	3165	0.13365681238004143	No Hit
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	2427	0.10249133764497964	TruSeq Adapter, Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.445928112482871E-5	0.0	0.0	0.05337826567089174	0.0
2	8.445928112482871E-5	0.0	0.0	0.1567564257676821	0.0
3	8.445928112482871E-5	0.0	0.0	0.32647735118802534	0.0
4	8.445928112482871E-5	0.0	0.0	0.4907928826163796	0.0
5	8.445928112482871E-5	0.0	0.0	0.8847531994231431	0.0
6	8.445928112482871E-5	0.0	0.0	1.7926482418744893	0.0
7	8.445928112482871E-5	0.0	0.0	2.3645642640112667	0.0
8	8.445928112482871E-5	0.0	0.0	3.324021697589321	0.0
9	8.445928112482871E-5	0.0	0.0	3.9468244366038077	0.0
10	8.445928112482871E-5	0.0	0.0	4.605100073690723	0.0
11	8.445928112482871E-5	0.0	0.0	5.047666706784825	0.0
12	8.445928112482871E-5	0.0	0.0	5.385377142362453	0.0
13	8.445928112482871E-5	0.0	0.0	5.634447562399573	0.0
14	8.445928112482871E-5	0.0	0.0	5.817766432081013	0.0
15	8.445928112482871E-5	0.0	0.0	5.942723938505197	0.0
16	8.445928112482871E-5	0.0	0.0	6.083855397264786	0.0
17	8.445928112482871E-5	0.0	0.0	6.230687857500301	0.0
18	8.445928112482871E-5	0.0	0.0	6.433812428605514	0.0
19	8.445928112482871E-5	0.0	0.0	6.565611136800809	0.0
20	8.445928112482871E-5	0.0	0.0	6.706531447357586	0.0
21	8.445928112482871E-5	0.0	0.0	6.85720680488428	0.0
22	8.445928112482871E-5	0.0	0.0	7.032079746453238	0.0
23	8.445928112482871E-5	0.0	0.0	7.214596252963993	0.0
24	8.445928112482871E-5	0.0	0.0	7.375153346382293	0.0
25	8.445928112482871E-5	0.0	0.0	7.519916554230249	0.0
26	8.445928112482871E-5	0.0	0.0	7.663666250704707	0.0
27	8.445928112482871E-5	0.0	0.0	7.82620813722944	0.0
28	8.445928112482871E-5	0.0	0.0	7.988032119864612	0.0
29	8.445928112482871E-5	0.0	0.0	8.164383098853254	0.0
30	8.445928112482871E-5	0.0	0.0	8.379036361832005	0.0
31	8.445928112482871E-5	0.0	0.0	8.553402547714215	0.0
32	8.445928112482871E-5	0.0	0.0	8.737481550925779	0.0
33	8.445928112482871E-5	0.0	0.0	8.916112930504791	0.0
34	8.445928112482871E-5	0.0	0.0	9.101627741495479	0.0
35	8.445928112482871E-5	0.0	0.0	9.296221925207083	0.0
36	8.445928112482871E-5	0.0	0.0	9.501457978340417	0.0
37	8.445928112482871E-5	0.0	0.0	9.700528503951638	0.0
38	8.445928112482871E-5	0.0	0.0	9.946389471306015	0.0
39	8.445928112482871E-5	0.0	0.0	10.289716449078444	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	25830	0.0	43.81533	1
TATCGCG	95	0.0	40.263157	1
TAGGGAC	2680	0.0	39.794773	5
CGGTCTA	230	0.0	39.130432	31
GTTTTTT	29705	0.0	38.46322	2
TAAGGGA	3305	0.0	38.39637	4
TAGGGAT	3510	0.0	38.141026	5
GGGCGAT	3980	0.0	38.103016	7
CACGACC	325	0.0	38.07692	27
ATAGGGA	3370	0.0	37.922848	4
GGCGATA	870	0.0	37.75862	8
AGGGCGA	2060	0.0	37.682037	6
AGGGATT	4120	0.0	37.627426	6
TTAGGGA	3040	0.0	37.598682	4
TAGCGGG	1235	0.0	37.348175	3
AAGGGAT	3385	0.0	37.090103	5
TAGGGCG	910	0.0	37.08791	5
CGTAAGG	390	0.0	36.923077	2
TACGGCG	470	0.0	36.861702	15
ACGGGAT	1115	0.0	36.72646	5
>>END_MODULE