FastQCFastQC Report
Thu 26 May 2016
SRR1547434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547434_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3377160
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165580.4902936194909332No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42030.12445368297622854No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC253.8924187E-545.031
CATTACG302.1667583E-644.99999642
TAGTACG1200.043.1249961
CGCATCG1200.041.24999621
GTTAGCG2250.041.01
CGTTTTT90500.040.8232041
CGACGAA451.9301297E-840.037
CTCACGA4700.039.25531824
CGGCATA750.039.034
TTTACGA1100.038.86363615
ACGGGAT11700.038.8461575
TTTCGCG1050.038.571431
TTACGAG1750.038.5714261
TACGTAG2400.038.4374961
CGGGATA7050.038.2978746
ATAGGGC16650.037.9729734
AGGGCGA28700.037.9442526
ATACGAG2200.037.8409081
GGGCGAT50050.037.672337
ACGGGTA3600.037.55
Produced by FastQC (version 0.11.5)