##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547434_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3377160
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270813938338723	31.0	31.0	33.0	30.0	34.0
2	31.666348944083193	31.0	31.0	34.0	30.0	34.0
3	31.819278032429615	31.0	31.0	34.0	30.0	34.0
4	35.56906098615405	37.0	35.0	37.0	33.0	37.0
5	35.43280093332859	37.0	35.0	37.0	33.0	37.0
6	35.47249819374859	37.0	35.0	37.0	33.0	37.0
7	35.88262771085764	37.0	35.0	37.0	35.0	37.0
8	35.914952208364426	37.0	35.0	37.0	35.0	37.0
9	37.65758625590733	39.0	37.0	39.0	35.0	39.0
10	36.95203454974002	39.0	37.0	39.0	32.0	39.0
11	36.67451556929491	39.0	35.0	39.0	32.0	39.0
12	36.11403635006929	38.0	35.0	39.0	32.0	39.0
13	35.87341109097585	38.0	35.0	39.0	31.0	39.0
14	36.90145151547454	39.0	35.0	41.0	31.0	41.0
15	37.110554134242975	39.0	35.0	41.0	32.0	41.0
16	37.20290836087126	39.0	35.0	41.0	32.0	41.0
17	37.14524008338367	39.0	35.0	41.0	32.0	41.0
18	37.049497506780845	39.0	35.0	41.0	32.0	41.0
19	37.01226207819588	39.0	35.0	41.0	31.0	41.0
20	36.87145293678712	38.0	35.0	41.0	31.0	41.0
21	36.78852941524831	38.0	35.0	41.0	31.0	41.0
22	36.70443893685819	38.0	35.0	41.0	31.0	41.0
23	36.66155231022516	38.0	35.0	40.0	31.0	41.0
24	36.56359633538239	38.0	35.0	40.0	31.0	41.0
25	36.455987871465965	38.0	35.0	40.0	31.0	41.0
26	36.303797273448694	38.0	35.0	40.0	30.0	41.0
27	36.21554975186251	38.0	35.0	40.0	30.0	41.0
28	36.1385936704213	38.0	35.0	40.0	30.0	41.0
29	36.19601055324592	38.0	35.0	40.0	30.0	41.0
30	36.143199315401105	38.0	35.0	40.0	30.0	41.0
31	36.01639780170321	38.0	35.0	40.0	30.0	41.0
32	35.96521041348352	38.0	35.0	40.0	30.0	41.0
33	35.879050444752394	38.0	35.0	40.0	29.0	41.0
34	35.76363749422592	38.0	35.0	40.0	29.0	41.0
35	35.6671229672269	38.0	34.0	40.0	29.0	41.0
36	35.60650783498561	38.0	34.0	40.0	28.0	41.0
37	35.55881894846557	38.0	34.0	40.0	28.0	41.0
38	35.44798499330798	38.0	34.0	40.0	27.0	41.0
39	35.40187672482204	38.0	34.0	40.0	27.0	41.0
40	35.28540756138294	38.0	34.0	40.0	27.0	41.0
41	35.20939250731384	38.0	34.0	40.0	27.0	41.0
42	35.16937693209679	37.0	34.0	40.0	27.0	41.0
43	35.140048739177296	37.0	34.0	40.0	27.0	41.0
44	35.01829406957325	37.0	34.0	40.0	26.0	41.0
45	34.890578178114154	37.0	34.0	40.0	26.0	41.0
46	34.87180708050551	37.0	34.0	40.0	26.0	41.0
47	34.80785097537576	37.0	34.0	40.0	26.0	41.0
48	34.73564563124045	37.0	34.0	40.0	26.0	41.0
49	34.67730104584918	36.0	34.0	40.0	26.0	41.0
50	34.566788070449725	36.0	33.0	40.0	26.0	41.0
51	34.003848203816226	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	5.0
12	2.0
13	11.0
14	23.0
15	94.0
16	226.0
17	580.0
18	1443.0
19	2729.0
20	4576.0
21	7682.0
22	11938.0
23	17454.0
24	25022.0
25	35426.0
26	46175.0
27	55347.0
28	63507.0
29	74702.0
30	89767.0
31	109500.0
32	136019.0
33	173784.0
34	276043.0
35	383139.0
36	264596.0
37	327285.0
38	471129.0
39	798525.0
40	428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.45377180826493	23.871270535005745	28.870234161247915	12.80472349548141
2	31.053873668999987	25.374219758613748	29.766194080233095	13.80571249215317
3	29.318599059564843	26.15688922052849	30.72649800424025	13.798013715666418
4	26.475055964182925	28.383434601854812	29.859674993189543	15.281834440772721
5	23.383789930000358	32.10351301093226	29.521136102524014	14.991560956543367
6	21.731691717300926	40.54984069454808	26.990400217934596	10.728067370216394
7	88.34354901751767	3.950538322140497	5.968654135427401	1.737258524914425
8	88.67119117838658	3.1269765128095797	5.931848061684966	2.269984247118881
9	83.63157209015858	5.340285920714447	7.7978242073221296	3.2303177818048296
10	42.64381906690829	28.335613355600564	15.19670373923652	13.823863838254628
11	35.92835400158713	25.07225005626029	23.204911819398546	15.79448412275403
12	33.343963567020815	22.586522403439577	26.332243660353672	17.737270369185943
13	23.658103258359095	31.20488813085551	27.41270771891175	17.724300891873646
14	19.094505442442763	33.217525968565305	29.341280839521964	18.34668774946997
15	18.120935934335357	26.328216608037526	38.760615428348075	16.79023202927904
16	21.05591680583686	23.780010422958934	36.84735102867498	18.316721742529225
17	21.948886046263723	23.592397162112544	28.026270594227103	26.43244619739663
18	23.028669059209513	25.030528609837855	31.84068862594606	20.10011370500657
19	26.83476649018702	26.363986308022124	26.813979793672786	19.987267408118065
20	28.27775408923474	26.666429781236307	26.11839533809473	18.937420791434224
21	24.408052920205144	27.438054459960444	29.040436342962728	19.11345627687169
22	23.476412133271744	24.800808963744686	28.723809354605645	22.998969548377925
23	21.872668159044878	28.147170995747906	28.06088547773869	21.919275367468526
24	21.723311895201885	26.0210946475737	33.257974155799545	18.997619301424866
25	21.579996209833112	26.538037877980315	30.7730756019851	21.108890310201474
26	19.649379952386028	31.486219190088715	27.2630849589596	21.60131589856566
27	19.878004003363774	30.862262966516248	29.235837212332257	20.023895817787725
28	18.33336294401213	28.79899086806666	33.23943787087375	19.62820831704746
29	20.13043504009286	26.961825912897226	32.14795863980386	20.759780407206055
30	21.65520733397292	28.540993023724077	30.145951035781543	19.657848606521455
31	25.571456490068577	27.49766075637518	26.740574920939487	20.190307832616757
32	26.95314998401023	27.421502090513922	27.190686849305333	18.43466107617051
33	25.666447547643585	28.14936218597875	26.475352070970875	19.70883819540679
34	21.03270203366142	27.85147875729903	29.671232633336885	21.44458657570266
35	21.670989825770768	28.313642231934526	29.01141787774343	21.00395006455128
36	26.026128462968884	28.047264565492902	26.663350270641605	19.263256700896612
37	22.31380805173578	30.573884565729788	27.792109346314653	19.320198036219782
38	21.81489772471544	30.7934477489962	26.287353871300144	21.104300654988215
39	21.785020549811087	28.81595778701631	27.695785808193868	21.70323585497874
40	23.94026341659856	26.72162408651056	27.250263535041274	22.087848961849602
41	19.858934726219662	25.773075601985102	29.5261403072404	24.841849364554832
42	22.628184628504425	26.551836454298876	27.016131897807625	23.80384701938907
43	21.888361818806334	26.81892477703159	27.731673950893647	23.561039453268425
44	21.60839285079771	28.744773715192647	28.285808193867034	21.361025240142606
45	20.34999822335927	31.191652157434056	26.305357164007624	22.152992455199044
46	21.966622842862048	29.74001824017814	27.365093747409063	20.92826516955075
47	21.965349583673856	28.070420116310746	28.03014959314927	21.934080706866123
48	21.790054365206267	26.73382368617418	29.74368996434874	21.732431984270807
49	21.41811462885975	26.68286370796764	29.939416551185023	21.95960511198759
50	20.59914247474209	29.568335524523565	28.90437527389878	20.928146726835568
51	20.01302869866989	30.007076952232055	27.129481576235655	22.850412772862406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3600.0
1	4377.5
2	5155.0
3	15622.0
4	26089.0
5	18151.0
6	10213.0
7	10789.5
8	11366.0
9	12904.0
10	14442.0
11	14911.0
12	15380.0
13	15121.5
14	14863.0
15	14971.0
16	15079.0
17	13924.0
18	12769.0
19	13250.5
20	13732.0
21	14475.0
22	15218.0
23	16885.0
24	18552.0
25	18027.5
26	26677.0
27	35851.0
28	40904.0
29	45957.0
30	53693.5
31	61430.0
32	69660.0
33	77890.0
34	85897.0
35	93904.0
36	105246.0
37	116588.0
38	132509.5
39	148431.0
40	175655.0
41	202879.0
42	233328.5
43	263778.0
44	281126.5
45	298475.0
46	303611.0
47	308747.0
48	293561.5
49	278376.0
50	261264.5
51	244153.0
52	224351.5
53	204550.0
54	191961.5
55	179373.0
56	167431.5
57	155490.0
58	142212.0
59	128934.0
60	115668.5
61	102403.0
62	90966.5
63	79530.0
64	67204.0
65	54878.0
66	45601.5
67	36325.0
68	30605.0
69	24885.0
70	20321.0
71	15757.0
72	12879.5
73	10002.0
74	8498.5
75	5652.0
76	4309.0
77	3004.5
78	1700.0
79	1334.5
80	969.0
81	707.0
82	445.0
83	275.0
84	105.0
85	74.5
86	44.0
87	34.0
88	24.0
89	20.0
90	16.0
91	8.5
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3377160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.39787944679978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60188574928823	12.725063673611583
2	8.902622270131317	2.919682534920167
3	3.2998898088297257	1.6233358581873902
4	1.6708252914946506	1.0959196682637353
5	0.9577275882357408	0.7852350767381987
6	0.6630110151994304	0.6523184819484359
7	0.5078405562477495	0.5829255753682437
8	0.36248627848751447	0.47552050366058846
9	0.2950372624734686	0.4354186916118387
>10	2.72041579924906	10.29988772747096
>50	1.1884072303576783	14.414305032677019
>100	1.8183196415634921	51.85490496719793
>500	0.008602871377402158	0.9215997808399444
>1k	0.0027455972481070716	0.7168998783703655
>5k	0.0	0.0
>10k+	1.8303981654047143E-4	0.49698254913362
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16558	0.4902936194909332	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4203	0.12445368297622854	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.96106787952007E-5	0.0	0.0	0.05128569567328761	0.0
2	2.96106787952007E-5	0.0	0.0	0.17559132525554016	0.0
3	2.96106787952007E-5	0.0	0.0	0.2789029835719954	0.0
4	2.96106787952007E-5	0.0	0.0	0.47850856933044333	0.0
5	2.96106787952007E-5	0.0	0.0	0.8097040116547631	0.0
6	2.96106787952007E-5	0.0	0.0	1.2728742493692926	0.0
7	2.96106787952007E-5	0.0	0.0	1.4975600800672755	0.0
8	2.96106787952007E-5	0.0	0.0	2.0702602186452523	0.0
9	2.96106787952007E-5	0.0	0.0	2.283990098189011	0.0
10	2.96106787952007E-5	0.0	0.0	2.664043160525412	0.0
11	2.96106787952007E-5	0.0	0.0	3.102221985336792	0.0
12	2.96106787952007E-5	0.0	0.0	3.432173779151713	0.0
13	5.92213575904014E-5	0.0	0.0	3.589880254414952	0.0
14	5.92213575904014E-5	0.0	0.0	3.657718319534757	0.0
15	5.92213575904014E-5	0.0	0.0	3.757861635220126	0.0
16	5.92213575904014E-5	0.0	0.0	3.972983216667259	0.0
17	5.92213575904014E-5	0.0	0.0	4.2055750986035605	0.0
18	5.92213575904014E-5	0.0	0.0	4.49987563514906	0.0
19	5.92213575904014E-5	0.0	0.0	4.649794501889161	0.0
20	5.92213575904014E-5	0.0	0.0	4.820529675822288	0.0
21	5.92213575904014E-5	0.0	0.0	5.046962536569188	0.0
22	5.92213575904014E-5	0.0	0.0	5.277925831171753	0.0
23	5.92213575904014E-5	0.0	0.0	5.529202051427827	0.0
24	5.92213575904014E-5	0.0	0.0	5.720042876262895	0.0
25	5.92213575904014E-5	0.0	0.0	5.882042899951439	0.0
26	5.92213575904014E-5	0.0	0.0	6.043213824633716	0.0
27	5.92213575904014E-5	0.0	0.0	6.211106373402504	0.0
28	5.92213575904014E-5	0.0	0.0	6.376393182437314	0.0
29	5.92213575904014E-5	0.0	0.0	6.572415876061543	0.0
30	5.92213575904014E-5	0.0	0.0	6.8208790818320715	0.0
31	8.88320363856021E-5	0.0	0.0	7.032032832320648	0.0
32	8.88320363856021E-5	0.0	0.0	7.235547027680062	0.0
33	8.88320363856021E-5	0.0	0.0	7.431036729085978	0.0
34	8.88320363856021E-5	0.0	0.0	7.6479645619396175	0.0
35	8.88320363856021E-5	0.0	0.0	7.896131660922195	0.0
36	8.88320363856021E-5	0.0	0.0	8.11154934915728	0.0
37	8.88320363856021E-5	0.0	0.0	8.333303722654538	0.0
38	8.88320363856021E-5	0.0	0.0	8.582299920643381	0.0
39	8.88320363856021E-5	0.0	0.0	8.938960546731574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	25	3.8924187E-5	45.0	31
CATTACG	30	2.1667583E-6	44.999996	42
TAGTACG	120	0.0	43.124996	1
CGCATCG	120	0.0	41.249996	21
GTTAGCG	225	0.0	41.0	1
CGTTTTT	9050	0.0	40.823204	1
CGACGAA	45	1.9301297E-8	40.0	37
CTCACGA	470	0.0	39.255318	24
CGGCATA	75	0.0	39.0	34
TTTACGA	110	0.0	38.863636	15
ACGGGAT	1170	0.0	38.846157	5
TTTCGCG	105	0.0	38.57143	1
TTACGAG	175	0.0	38.571426	1
TACGTAG	240	0.0	38.437496	1
CGGGATA	705	0.0	38.297874	6
ATAGGGC	1665	0.0	37.972973	4
AGGGCGA	2870	0.0	37.944252	6
ATACGAG	220	0.0	37.840908	1
GGGCGAT	5005	0.0	37.67233	7
ACGGGTA	360	0.0	37.5	5
>>END_MODULE