FastQCFastQC Report
Thu 26 May 2016
SRR1547433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547433_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1033513
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99980.9673801877673527No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC207.032955E-445.00000424
CGTTAAT207.032955E-445.00000423
GTATACG207.032955E-445.0000041
GTACGTT207.032955E-445.0000049
TCGTTAG253.8905317E-545.01
TAGGACG453.8562575E-1045.01
CGCGTAA302.1652886E-644.99999631
AATCGCG302.1652886E-644.9999961
CGGTCTA2000.042.7531
CGTTTTT49650.041.7371641
GCGTAAG650.041.538461
CGACGGT2200.040.90909228
CGATCGT403.4577715E-739.37500410
AGGGCGA10150.039.014786
TCACGAC2250.039.025
TACGGGA3700.038.918924
TATTGCG700.038.5714261
TGCGCGA356.2476793E-638.57142638
ACGTAGG700.038.5714262
GCGTAAC356.2476793E-638.57142632
Produced by FastQC (version 0.11.5)