##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547432_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2366533
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27743327475256	31.0	31.0	33.0	30.0	34.0
2	31.679743320714312	31.0	31.0	34.0	30.0	34.0
3	31.837138548247584	31.0	31.0	34.0	30.0	34.0
4	35.579961487965726	37.0	35.0	37.0	33.0	37.0
5	35.4545560953513	37.0	35.0	37.0	33.0	37.0
6	35.503831976989126	37.0	35.0	37.0	33.0	37.0
7	35.887372793871876	37.0	35.0	37.0	35.0	37.0
8	35.912161377001716	37.0	35.0	37.0	35.0	37.0
9	37.650163340211186	39.0	37.0	39.0	35.0	39.0
10	36.99237534401591	39.0	37.0	39.0	32.0	39.0
11	36.680419415237395	39.0	35.0	39.0	32.0	39.0
12	36.00130105939786	37.0	35.0	39.0	31.0	39.0
13	35.67711796116936	38.0	35.0	39.0	30.0	39.0
14	36.768106339527066	39.0	35.0	41.0	31.0	41.0
15	37.02280804873627	39.0	35.0	41.0	32.0	41.0
16	37.12233972651131	39.0	35.0	41.0	32.0	41.0
17	37.05814159363085	38.0	35.0	41.0	32.0	41.0
18	37.002022790301254	38.0	35.0	41.0	32.0	41.0
19	36.9217843148606	38.0	35.0	41.0	32.0	41.0
20	36.76936894604893	38.0	35.0	41.0	31.0	41.0
21	36.67405567553886	38.0	35.0	41.0	31.0	41.0
22	36.56895678192529	38.0	35.0	40.0	31.0	41.0
23	36.51049700130951	38.0	35.0	40.0	31.0	41.0
24	36.398885627202326	38.0	35.0	40.0	31.0	41.0
25	36.31117630728158	38.0	35.0	40.0	31.0	41.0
26	36.13822794780381	38.0	35.0	40.0	30.0	41.0
27	36.05306412376248	38.0	35.0	40.0	30.0	41.0
28	35.99059256727035	38.0	35.0	40.0	30.0	41.0
29	36.02255282305381	38.0	35.0	40.0	30.0	41.0
30	36.01768832296021	38.0	35.0	40.0	30.0	41.0
31	35.91612455858422	38.0	35.0	40.0	30.0	41.0
32	35.80603017156321	38.0	34.0	40.0	30.0	41.0
33	35.73897427164548	38.0	34.0	40.0	29.0	41.0
34	35.67058562039912	38.0	34.0	40.0	29.0	41.0
35	35.55986542338518	38.0	34.0	40.0	29.0	41.0
36	35.45161381649865	38.0	34.0	40.0	28.0	41.0
37	35.37013513016721	38.0	34.0	40.0	27.0	41.0
38	35.292003111725045	37.0	34.0	40.0	27.0	41.0
39	35.25269751150734	37.0	34.0	40.0	27.0	41.0
40	35.099757746881195	37.0	34.0	40.0	27.0	41.0
41	35.04878782590397	37.0	34.0	40.0	26.0	41.0
42	34.98873161709556	37.0	34.0	40.0	26.0	41.0
43	34.963741895845104	37.0	34.0	40.0	27.0	41.0
44	34.83110186927459	37.0	34.0	40.0	26.0	41.0
45	34.703118443731825	36.0	34.0	40.0	26.0	41.0
46	34.66860888903725	36.0	34.0	40.0	26.0	41.0
47	34.625054879860116	36.0	33.0	40.0	26.0	41.0
48	34.55785066170639	36.0	34.0	40.0	26.0	41.0
49	34.48884972235756	36.0	33.0	40.0	26.0	41.0
50	34.374912160531885	36.0	33.0	40.0	25.0	41.0
51	33.78798732153746	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	3.0
13	9.0
14	31.0
15	62.0
16	177.0
17	455.0
18	1006.0
19	1998.0
20	3539.0
21	5617.0
22	8399.0
23	12721.0
24	17921.0
25	25222.0
26	33378.0
27	40065.0
28	46156.0
29	54068.0
30	64031.0
31	77610.0
32	95692.0
33	123212.0
34	204434.0
35	301136.0
36	180736.0
37	218765.0
38	316266.0
39	533552.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.17591599187503	24.510877304478747	29.080008603302804	13.233198100343413
2	29.47658029699987	26.55796475265716	30.116588274915244	13.848866675427725
3	29.67209838189453	26.11330583600567	30.929084868032685	13.28551091406712
4	26.538231243764614	28.644899521789892	29.862376734235273	14.954492500210224
5	23.750059686469616	32.64991445291487	29.162365367396102	14.437660493219406
6	21.862361522108504	42.22751172284519	25.625841684861356	10.28428507018495
7	89.01899952377592	4.022720156448273	5.579639075390032	1.3786412443857745
8	89.61894890119851	3.3084262928089316	5.22621066344733	1.846414142545234
9	84.31359292264253	6.31818783004505	6.930475932513936	2.4377433147984835
10	44.714652193736576	32.33920676364961	12.285651626239735	10.660489416374078
11	39.23528638730159	21.67719613459859	23.465677427696974	15.621840050402847
12	36.29837403492789	21.72046618407603	25.611221140799646	16.369938640196438
13	22.10267086915754	34.8757021347262	25.78704797270944	17.234579023406816
14	17.234240976145273	37.69193161472922	27.730946494302	17.3428809148235
15	16.767228684324284	26.34207932025457	40.247315376544506	16.643376618876644
16	18.959465175427514	22.91947756485965	39.20558048419354	18.915476775519295
17	18.374643412958957	23.7277908231155	28.4031534738793	29.49441229004624
18	22.130686535957874	25.178731925563685	32.19612826020174	20.4944532782767
19	27.574768659469356	26.25587726856122	25.81810606486366	20.351248007105756
20	29.80562705020382	25.073218924054725	25.642448256584633	19.47870576915682
21	23.323655321941423	28.809908841330333	27.423703789467545	20.442732047260698
22	24.390870526631154	24.00427122714959	26.300795298438683	25.30406294778057
23	21.70707106133741	29.43166226712241	25.818866671202134	23.04240000033805
24	20.44243625590685	25.60475598692264	35.53633099559567	18.41647676157484
25	20.7661587647415	27.51421594374556	31.591953292009872	20.12767199950307
26	18.791751477794733	34.32422028342727	26.078740503512943	20.80528773526505
27	19.907476464515813	32.374110143403875	27.734411478732813	19.9840019133475
28	17.49711497790227	29.665759995740604	34.38988596398191	18.44723906237521
29	17.54735725214903	26.721326091797582	33.306951561630456	22.42436509442294
30	19.625418280666274	30.95566383397147	29.531977791985152	19.886940093377103
31	27.767455598548597	27.3263461781433	24.570753925679465	20.33544429762864
32	26.95990294663121	28.088135682029364	26.16511157883706	18.786849792502366
33	25.217058033841067	28.75637060628354	25.050062686639063	20.97650867323633
34	20.227395941658113	28.324895532832205	27.630546457623872	23.817162067885807
35	21.699042438875775	26.137983286098272	29.763455654326393	22.399518620699563
36	28.056190215813597	27.873348903226784	25.235270330056668	18.835190550902944
37	20.872559140312006	32.11537722060077	27.98008732605884	19.031976313028384
38	20.876277660188975	32.52234386759027	25.481664527813475	21.119713944407284
39	22.54225907688589	30.14561808350021	26.40622378813226	20.90589905148164
40	23.88025013807118	28.07482507110613	26.377405259085762	21.667519531736936
41	18.94695742675044	26.51968090028747	28.381476193232885	26.15188547972921
42	21.46811390333454	28.029273202613275	25.01684954319251	25.485763350859674
43	22.749313024580683	26.229763117607064	26.67961951090477	24.34130434690748
44	20.49458004599978	29.60968640623224	27.863545532642053	22.032188015125925
45	19.46547967004897	33.96766493431531	24.535808290017506	22.031047105618217
46	22.30845713962155	31.327896124837473	25.521385081044716	20.842261654496262
47	22.22791737955904	28.043851490767295	27.41816826555979	22.310062864113874
48	22.324767919990972	26.59882621539611	30.163872635623505	20.91253322898941
49	21.019609699083006	27.249355914326994	29.963452865436484	21.767581521153517
50	20.355220062428877	30.757737162338323	28.177549182707363	20.709493592525437
51	20.67606916954042	30.801429770892696	26.209522537822206	22.31297852174468
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3274.0
1	3822.0
2	4370.0
3	12373.0
4	20376.0
5	14225.5
6	8075.0
7	8433.5
8	8792.0
9	9441.0
10	10090.0
11	10561.0
12	11032.0
13	10770.0
14	10508.0
15	9966.5
16	9425.0
17	9038.0
18	8651.0
19	8328.0
20	8005.0
21	8416.5
22	8828.0
23	10414.0
24	12000.0
25	14217.5
26	18751.0
27	21067.0
28	29052.0
29	37037.0
30	44176.5
31	51316.0
32	52841.5
33	54367.0
34	60336.0
35	66305.0
36	73921.0
37	81537.0
38	90575.5
39	99614.0
40	122674.5
41	145735.0
42	171905.5
43	198076.0
44	207136.5
45	216197.0
46	201815.5
47	187434.0
48	187556.5
49	187679.0
50	182282.5
51	176886.0
52	159522.0
53	142158.0
54	130611.0
55	119064.0
56	112218.5
57	105373.0
58	97898.5
59	90424.0
60	80505.0
61	70586.0
62	63650.5
63	56715.0
64	47765.5
65	38816.0
66	32502.0
67	26188.0
68	22836.5
69	19485.0
70	16210.5
71	12936.0
72	11340.5
73	9745.0
74	7359.5
75	4172.5
76	3371.0
77	2685.0
78	1999.0
79	1479.5
80	960.0
81	688.0
82	416.0
83	272.0
84	128.0
85	79.5
86	31.0
87	27.5
88	24.0
89	17.5
90	11.0
91	11.0
92	11.0
93	7.0
94	3.0
95	1.5
96	0.0
97	1.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2366533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.888411911767161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96524976627869	11.905555771477
2	8.011302033741462	2.3855112925584168
3	2.6866847342253033	1.2000140700060895
4	1.2965131247987234	0.7721208580406311
5	0.7604394276933949	0.5660867716723872
6	0.5192236743272775	0.46382495626354503
7	0.35720199280737613	0.37227192832342143
8	0.2815608078363901	0.33535946282224766
9	0.22435430679134966	0.30062514003197344
>10	2.448579129615987	8.886717913513396
>50	1.3313130125546624	14.712743178154813
>100	2.0991992272848776	55.146993985109475
>500	0.015219912318124794	1.459092311724315
>1k	0.002871681569457508	0.9111898305584611
>5k	0.0	0.0
>10k+	2.871681569457508E-4	0.5818925297438371
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13610	0.5751029036992089	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3493	0.14759988557100195	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3236	0.13674011729394858	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2656	0.11223169083211602	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.267677230784443E-4	0.0	0.0	0.03536819473888596	0.0
2	1.267677230784443E-4	0.0	0.0	0.11776721473987474	0.0
3	1.267677230784443E-4	0.0	0.0	0.17274215064822676	0.0
4	1.267677230784443E-4	0.0	0.0	0.32503244197313114	0.0
5	1.267677230784443E-4	0.0	0.0	0.543622252468062	0.0
6	1.267677230784443E-4	0.0	0.0	0.8380614172715952	0.0
7	1.267677230784443E-4	0.0	0.0	0.9798722434886815	0.0
8	1.267677230784443E-4	0.0	0.0	1.3398925770314634	0.0
9	1.267677230784443E-4	0.0	0.0	1.4574062563251813	0.0
10	1.267677230784443E-4	0.0	0.0	1.6968282293126695	0.0
11	1.267677230784443E-4	0.0	0.0	1.9890700869161766	0.0
12	1.267677230784443E-4	0.0	0.0	2.22616798498056	0.0
13	1.267677230784443E-4	0.0	0.0	2.331342939227976	0.0
14	1.267677230784443E-4	0.0	0.0	2.3670491812284045	0.0
15	1.267677230784443E-4	0.0	0.0	2.424432703875247	0.0
16	1.267677230784443E-4	0.0	0.0	2.551496218307541	0.0
17	1.267677230784443E-4	0.0	0.0	2.7028568796632033	0.0
18	1.267677230784443E-4	0.0	0.0	2.896388936896295	0.0
19	1.267677230784443E-4	0.0	0.0	2.994464898651318	0.0
20	1.267677230784443E-4	0.0	0.0	3.0968087070833157	0.0
21	1.267677230784443E-4	0.0	0.0	3.234436198438813	0.0
22	1.267677230784443E-4	0.0	0.0	3.3768808632712917	0.0
23	1.267677230784443E-4	0.0	0.0	3.5345376548731835	0.0
24	1.267677230784443E-4	0.0	0.0	3.664009756043968	0.0
25	1.267677230784443E-4	0.0	0.0	3.7662690526605798	0.0
26	1.267677230784443E-4	0.0	0.0	3.864091478969446	0.0
27	1.267677230784443E-4	0.0	0.0	3.960096901247521	0.0
28	1.267677230784443E-4	0.0	0.0	4.064299969618002	0.0
29	1.267677230784443E-4	0.0	0.0	4.179193782634766	0.0
30	1.267677230784443E-4	0.0	0.0	4.322652589251872	0.0
31	1.267677230784443E-4	0.0	0.0	4.459477218361206	0.0
32	1.267677230784443E-4	0.0	0.0	4.577497968547237	0.0
33	1.267677230784443E-4	0.0	0.0	4.698054073194839	0.0
34	1.267677230784443E-4	0.0	0.0	4.827526174365623	0.0
35	1.267677230784443E-4	0.0	0.0	4.994394753844548	0.0
36	1.267677230784443E-4	0.0	0.0	5.1351491823693145	0.0
37	1.267677230784443E-4	0.0	0.0	5.2771712881248645	0.0
38	1.267677230784443E-4	0.0	0.0	5.418390531634252	0.0
39	1.267677230784443E-4	0.0	0.0	5.562652200497521	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTT	20	7.034803E-4	45.0	13
CGCATTA	25	3.892063E-5	45.0	16
CGCATCG	100	0.0	45.0	21
TCGATAG	90	0.0	45.0	1
CCGTCGA	20	7.034803E-4	45.0	43
ATATCGA	25	3.892063E-5	45.0	16
ACGATAC	35	1.2126657E-7	45.0	20
TACGATA	35	1.2126657E-7	45.0	23
CGCTAAT	20	7.034803E-4	45.0	22
GCGATCG	25	3.892063E-5	45.0	9
TTACGAC	20	7.034803E-4	45.0	18
ACCCGTA	20	7.034803E-4	45.0	33
ACGTAAC	20	7.034803E-4	45.0	44
CACGTAA	20	7.034803E-4	45.0	23
TTACCCG	20	7.034803E-4	45.0	24
TACCGTT	70	0.0	45.0	14
CGAAGTA	40	6.8212103E-9	45.0	21
CGTATCA	65	0.0	44.999996	38
ATTACGT	30	2.16648E-6	44.999996	33
GAACGTA	30	2.16648E-6	44.999996	36
>>END_MODULE