FastQCFastQC Report
Thu 26 May 2016
SRR1547431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547431_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1019675
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80530.7897614435972247No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21360.20947851030965747No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19800.19417951798367125No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14130.13857356510652905No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13070.12817809596194868No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12810.1256282639076176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG253.8904946E-545.01
AATCCCG253.8904946E-545.023
ATTAGCG253.8904946E-545.01
AGTCACG207.0329127E-445.01
TCTTCGA253.8904946E-545.026
GACCGTT253.8904946E-545.037
CGCATCA207.0329127E-445.018
CGTTCGA207.0329127E-445.014
ATCGTAG207.0329127E-445.01
CGCCTAT253.8904946E-545.032
CCCGTGA207.0329127E-445.011
CCGTAGA253.8904946E-545.012
TACGATT207.0329127E-445.044
CGGATGT253.8904946E-545.06
TGTTACG253.8904946E-545.01
TCCGTTA207.0329127E-445.045
CATGTCG207.0329127E-445.013
TAGATCA253.8904946E-545.033
CGGTCCC207.0329127E-445.028
ATACGGA207.0329127E-445.04