##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547431_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1019675
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19081275896732	31.0	31.0	33.0	30.0	34.0
2	31.583466300536937	31.0	31.0	34.0	30.0	34.0
3	31.709715350479318	31.0	31.0	34.0	30.0	34.0
4	35.47888788094246	37.0	35.0	37.0	33.0	37.0
5	35.347049795277904	37.0	35.0	37.0	33.0	37.0
6	35.395460318238655	37.0	35.0	37.0	33.0	37.0
7	35.8308716012455	37.0	35.0	37.0	35.0	37.0
8	35.87265942579744	37.0	35.0	37.0	35.0	37.0
9	37.62054576213009	39.0	37.0	39.0	35.0	39.0
10	36.881513227253784	39.0	37.0	39.0	32.0	39.0
11	36.59263883100007	39.0	35.0	39.0	32.0	39.0
12	35.95574472258318	37.0	35.0	39.0	31.0	39.0
13	35.644477897369256	37.0	35.0	39.0	30.0	39.0
14	36.693284625003066	38.0	35.0	41.0	31.0	41.0
15	36.92427979503273	38.0	35.0	41.0	31.0	41.0
16	37.0450006129404	38.0	35.0	41.0	32.0	41.0
17	36.97125162429205	38.0	35.0	41.0	32.0	41.0
18	36.90351239365484	38.0	35.0	40.0	32.0	41.0
19	36.818059675877116	38.0	35.0	40.0	31.0	41.0
20	36.64176919116385	38.0	35.0	40.0	31.0	41.0
21	36.53478118027803	38.0	35.0	40.0	31.0	41.0
22	36.439753843136295	38.0	35.0	40.0	31.0	41.0
23	36.387169441243536	38.0	35.0	40.0	31.0	41.0
24	36.259337534018194	38.0	35.0	40.0	30.0	41.0
25	36.13880010787751	38.0	35.0	40.0	30.0	41.0
26	35.99274866991934	38.0	35.0	40.0	30.0	41.0
27	35.89383480030402	37.0	34.0	40.0	30.0	41.0
28	35.81447814254542	37.0	34.0	40.0	30.0	41.0
29	35.80254688994042	37.0	34.0	40.0	30.0	41.0
30	35.76516144850075	37.0	34.0	40.0	30.0	41.0
31	35.64781131242798	37.0	34.0	40.0	29.0	41.0
32	35.50954961139579	37.0	34.0	40.0	29.0	41.0
33	35.42138034177557	37.0	34.0	40.0	28.0	41.0
34	35.29821560791429	37.0	34.0	40.0	27.0	41.0
35	35.20821143991958	37.0	34.0	40.0	27.0	41.0
36	35.14253953465565	37.0	34.0	40.0	27.0	41.0
37	35.084223894868465	37.0	34.0	40.0	26.0	41.0
38	35.00652168583127	37.0	34.0	40.0	26.0	41.0
39	34.976115919288006	37.0	34.0	40.0	26.0	41.0
40	34.86113908843504	37.0	34.0	40.0	26.0	41.0
41	34.820842915634884	37.0	34.0	40.0	26.0	41.0
42	34.77376811238875	36.0	34.0	40.0	26.0	41.0
43	34.720657072106306	36.0	33.0	40.0	26.0	41.0
44	34.55561428886655	36.0	33.0	40.0	25.0	41.0
45	34.41075931056464	36.0	33.0	40.0	24.0	41.0
46	34.370572976683746	36.0	33.0	40.0	24.0	41.0
47	34.326842376247335	35.0	33.0	40.0	24.0	41.0
48	34.25232451516415	35.0	33.0	40.0	24.0	41.0
49	34.20179272807512	36.0	33.0	40.0	24.0	41.0
50	34.05989163213769	35.0	33.0	40.0	24.0	41.0
51	33.50386054380072	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	4.0
12	2.0
13	7.0
14	14.0
15	34.0
16	80.0
17	203.0
18	484.0
19	885.0
20	1599.0
21	2628.0
22	4249.0
23	6105.0
24	8868.0
25	12252.0
26	15610.0
27	18227.0
28	20831.0
29	24058.0
30	28909.0
31	35008.0
32	43188.0
33	55642.0
34	88328.0
35	131487.0
36	83766.0
37	95533.0
38	131309.0
39	210193.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.43418246009758	24.858901120455045	28.402186971338907	13.304729448108466
2	30.44901561772133	26.336528795939884	29.71750802951921	13.496947556819574
3	29.32257827248878	25.79743545737612	31.697060337852744	13.182925932282345
4	26.154215804055212	27.68146713413588	30.719984308725817	15.44433275308309
5	22.100669330914265	32.8252629514306	30.21756932355898	14.856498394096157
6	19.240051977345722	42.49559908794469	27.896045308554196	10.368303626155392
7	87.78895236227228	3.7787530340549687	6.5676808787113545	1.8646137249613848
8	88.47093436634222	3.293598450486675	6.116850957412901	2.118616225758207
9	84.0802216392478	5.414960649226469	7.830926520705127	2.6738911908206044
10	43.175325471351165	29.15987937332974	14.868953342976928	12.795841812342168
11	36.9274523745311	24.072572143084805	22.284257238826097	16.715718243557998
12	34.53963272611371	22.968691004486725	26.32436805845	16.167308210949567
13	22.502365949935026	34.94956726407924	26.540809571677247	16.00725721430848
14	17.683379508176625	39.884178782455194	26.352121999656752	16.08031970971143
15	15.702356142888666	26.840709049452034	39.98793733297374	17.468997474685562
16	17.577659548385515	22.70429303454532	40.55753058572584	19.16051683134332
17	17.290313089955134	23.472773187535246	27.785519896045308	31.45139382646431
18	20.65972000882634	27.80464363645279	30.72675117071616	20.80888518400471
19	26.542084487704415	29.066418221492142	24.027263588888616	20.364233701914824
20	28.228602250717138	26.173437614926325	24.53428788584598	21.063672248510553
21	22.849927673033076	29.507343025964154	27.692156814671343	19.95057248633143
22	23.665285507637236	25.725844018927603	25.6850467060583	24.92382376737686
23	19.686468727780913	31.54897393777429	24.495942334567385	24.26861499987741
24	19.792482898962906	27.21671120700223	34.30946134797852	18.68134454605634
25	19.45051119229166	28.514968004511243	31.307818667712752	20.726702135484345
26	17.54617892956089	34.21354843455022	25.3065927869174	22.933679848971487
27	17.995930075759432	33.578247971167286	27.796013435653517	20.629808517419765
28	17.57844411209454	29.637188319807784	33.72309804594601	19.061269522151665
29	16.600093166940447	28.099541520582537	32.7181700051487	22.582195307328316
30	19.171794934660554	31.709024934415375	29.81655919778361	19.30262093314046
31	26.825017775271533	29.57285409566774	23.56142888665506	20.04069924240567
32	26.089097016206143	29.889131340868413	25.234510996150732	18.78726064677471
33	25.702601319047737	29.06377031897418	23.45639542010935	21.777232941868732
34	20.275676073258637	29.71270257680143	26.53727903498664	23.474342314953294
35	20.212126412827615	27.398828057959644	28.684629906587883	23.704415622624854
36	26.51903792875181	28.624218500992963	25.179885747909875	19.676857822345355
37	19.734523254958688	32.108465932772695	27.401377890013972	20.75563292225464
38	20.75190624463677	33.86824233211563	24.52585382597396	20.85399759727364
39	20.211636060509473	31.05391423737956	25.539608208497803	23.19484149361316
40	21.706327996665603	27.585652291171208	28.027852011670383	22.680167700492802
41	17.95542697428102	26.26366244146419	28.153872557432514	27.62703802682227
42	21.020325103586927	28.691789050432735	25.004928040797314	25.282957805183027
43	21.64101306788928	28.713462622894546	25.6731801799593	23.97234412925687
44	20.030990266506485	31.058327408242825	27.05960232426999	21.851080000980705
45	18.431068722877384	34.79579277711035	23.951357050040453	22.821781449971805
46	22.29720254002501	32.030892196042856	25.887562213450366	19.78434305048177
47	21.29560889499105	30.10282688111408	25.590310638193543	23.011253585701326
48	20.667173364062077	28.99718047417069	28.78250422928874	21.553141932478486
49	21.399367445509597	27.11971951847402	28.84879986270135	22.632113173315027
50	19.417167234658102	30.330546497658567	28.48799862701351	21.76428764066982
51	18.7391080491333	31.47032142594454	26.465099173756347	23.325471351165813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1579.0
1	1675.0
2	1771.0
3	6207.5
4	10644.0
5	7144.0
6	3644.0
7	4001.0
8	4358.0
9	5343.0
10	6328.0
11	6860.0
12	7392.0
13	7493.5
14	7595.0
15	7092.0
16	6589.0
17	6141.5
18	5694.0
19	5129.0
20	4564.0
21	4624.0
22	4684.0
23	4905.5
24	5127.0
25	5819.0
26	8343.5
27	10176.0
28	10580.0
29	10984.0
30	13775.0
31	16566.0
32	18951.0
33	21336.0
34	23791.5
35	26247.0
36	29614.5
37	32982.0
38	36372.0
39	39762.0
40	48266.5
41	56771.0
42	68724.0
43	80677.0
44	83127.5
45	85578.0
46	85572.5
47	85567.0
48	92162.0
49	98757.0
50	92466.5
51	86176.0
52	78714.0
53	71252.0
54	63517.0
55	55782.0
56	50115.0
57	44448.0
58	39806.0
59	35164.0
60	30874.0
61	26584.0
62	22423.0
63	18262.0
64	15322.5
65	12383.0
66	10450.0
67	8517.0
68	7105.0
69	5693.0
70	5073.5
71	4454.0
72	3926.0
73	3398.0
74	3083.5
75	2074.0
76	1379.0
77	1104.5
78	830.0
79	631.5
80	433.0
81	285.5
82	138.0
83	94.0
84	50.0
85	44.5
86	39.0
87	31.5
88	24.0
89	14.5
90	5.0
91	4.5
92	4.0
93	4.0
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1019675.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.397252649118336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73641532620951	16.633457187717
2	8.828205586966293	3.7779869076535166
3	3.202906836530804	2.0560022037851393
4	1.6332720770957476	1.3979014111347199
5	1.0157187956859337	1.0866795845875066
6	0.6459632838097411	0.8293103751438697
7	0.49011541802401537	0.7340986398681674
8	0.4006234246199307	0.6857792506998139
9	0.33382340938004995	0.642861344762549
>10	3.360874192045677	16.832423201248417
>50	1.310935535114873	20.427410045249168
>100	1.0355834460516369	32.89030636153839
>500	0.0023177785274208526	0.3016312720198985
>1k	0.0027813342329050233	0.9053897569870548
>5k	4.6355570548417053E-4	0.798762457604815
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8053	0.7897614435972247	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2136	0.20947851030965747	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1980	0.19417951798367125	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1413	0.13857356510652905	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1307	0.12817809596194868	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1281	0.1256282639076176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.80704636281168E-5	0.0	0.0	0.08169269620222129	0.0
2	9.80704636281168E-5	0.0	0.0	0.26224041974158435	0.0
3	9.80704636281168E-5	0.0	0.0	0.3875744722583176	0.0
4	9.80704636281168E-5	0.0	0.0	0.6421653958369088	0.0
5	9.80704636281168E-5	0.0	0.0	1.1499742565032975	0.0
6	9.80704636281168E-5	0.0	0.0	1.6687670090960356	0.0
7	9.80704636281168E-5	0.0	0.0	2.000049035231814	0.0
8	9.80704636281168E-5	0.0	0.0	2.7444038541692204	0.0
9	9.80704636281168E-5	0.0	0.0	2.9992889891386962	0.0
10	9.80704636281168E-5	0.0	0.0	3.4672812415720697	0.0
11	9.80704636281168E-5	0.0	0.0	4.083457964547527	0.0
12	1.961409272562336E-4	0.0	0.0	4.5749871282516485	0.0
13	1.961409272562336E-4	0.0	0.0	4.768284012062667	0.0
14	1.961409272562336E-4	0.0	0.0	4.843700198592689	0.0
15	1.961409272562336E-4	0.0	0.0	4.954814033883345	0.0
16	1.961409272562336E-4	0.0	0.0	5.212935494152549	0.0
17	1.961409272562336E-4	0.0	0.0	5.510677421727511	0.0
18	1.961409272562336E-4	0.0	0.0	5.887562213450364	0.0
19	2.942113908843504E-4	0.0	0.0	6.077916983352539	0.0
20	2.942113908843504E-4	0.0	0.0	6.262681736827911	0.0
21	2.942113908843504E-4	0.0	0.0	6.533258145977885	0.0
22	2.942113908843504E-4	0.0	0.0	6.80069630029176	0.0
23	2.942113908843504E-4	0.0	0.0	7.121484786819329	0.0
24	2.942113908843504E-4	0.0	0.0	7.354598278863364	0.0
25	2.942113908843504E-4	0.0	0.0	7.5457376124745625	0.0
26	2.942113908843504E-4	0.0	0.0	7.716870571505627	0.0
27	2.942113908843504E-4	0.0	0.0	7.883198077818913	0.0
28	2.942113908843504E-4	0.0	0.0	8.072768284012062	0.0
29	2.942113908843504E-4	0.0	0.0	8.274205016304215	0.0
30	2.942113908843504E-4	0.0	0.0	8.522617500674235	0.0
31	2.942113908843504E-4	0.0	0.0	8.74827763748253	0.0
32	2.942113908843504E-4	0.0	0.0	8.957363865937676	0.0
33	2.942113908843504E-4	0.0	0.0	9.171255547110599	0.0
34	3.922818545124672E-4	0.0	0.0	9.411724323926741	0.0
35	3.922818545124672E-4	0.0	0.0	9.667590163532498	0.0
36	3.922818545124672E-4	0.0	0.0	9.895211709613358	0.0
37	3.922818545124672E-4	0.0	0.0	10.133326795302425	0.0
38	3.922818545124672E-4	0.0	0.0	10.361046411846912	0.0
39	3.922818545124672E-4	0.0	0.0	10.57827248878319	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.8904946E-5	45.0	1
AATCCCG	25	3.8904946E-5	45.0	23
ATTAGCG	25	3.8904946E-5	45.0	1
AGTCACG	20	7.0329127E-4	45.0	1
TCTTCGA	25	3.8904946E-5	45.0	26
GACCGTT	25	3.8904946E-5	45.0	37
CGCATCA	20	7.0329127E-4	45.0	18
CGTTCGA	20	7.0329127E-4	45.0	14
ATCGTAG	20	7.0329127E-4	45.0	1
CGCCTAT	25	3.8904946E-5	45.0	32
CCCGTGA	20	7.0329127E-4	45.0	11
CCGTAGA	25	3.8904946E-5	45.0	12
TACGATT	20	7.0329127E-4	45.0	44
CGGATGT	25	3.8904946E-5	45.0	6
TGTTACG	25	3.8904946E-5	45.0	1
TCCGTTA	20	7.0329127E-4	45.0	45
CATGTCG	20	7.0329127E-4	45.0	13
TAGATCA	25	3.8904946E-5	45.0	33
CGGTCCC	20	7.0329127E-4	45.0	28
ATACGGA	20	7.0329127E-4	45.0	4
>>END_MODULE