FastQCFastQC Report
Thu 26 May 2016
SRR1547430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547430_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2656310
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390141.4687291769409443No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT41820.15743644378856383No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT29490.11101866875477637No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC27960.1052587988600728No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC253.8921935E-544.99999634
CGTTTTT195250.043.2368741
TAACGCG451.9299478E-840.01
TATTGCG2150.038.7209321
CGTTAGG3550.038.6619722
TAGGGAC35000.038.3142855
CGACCCG5250.037.71428332
TATGGGA36200.037.6657454
CGAGGGA19150.037.597914
TAGGGAT52850.037.2516565
TAGGGCG9800.037.193885
ATAGGGA47000.037.1010634
CACGACC6550.037.09923627
AGGGATT61200.036.9852946
GTTTTTT231600.036.858812
ACGGGAT16350.036.743125
TAAGGGA38950.036.739414
GGGCGAT46150.036.6630557
GGCGATT12350.036.0728768
TACGGGA12850.036.0700384