##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547430_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2656310
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238681855656907	31.0	31.0	33.0	30.0	34.0
2	31.641654400277076	31.0	31.0	34.0	30.0	34.0
3	31.678832666368006	31.0	31.0	34.0	30.0	34.0
4	35.49308439150551	37.0	35.0	37.0	33.0	37.0
5	35.39189928886312	37.0	35.0	37.0	33.0	37.0
6	35.4479842337679	37.0	35.0	37.0	33.0	37.0
7	35.90647815955216	37.0	35.0	37.0	35.0	37.0
8	35.95621934186898	37.0	35.0	37.0	35.0	37.0
9	37.682794929808644	39.0	37.0	39.0	35.0	39.0
10	37.012496658898996	39.0	37.0	39.0	33.0	39.0
11	36.698028091600754	39.0	35.0	39.0	32.0	39.0
12	36.411689147727486	39.0	35.0	39.0	32.0	39.0
13	36.32399719912209	39.0	35.0	39.0	32.0	39.0
14	37.422919011711734	40.0	36.0	41.0	32.0	41.0
15	37.54136866555485	40.0	36.0	41.0	32.0	41.0
16	37.57305962030034	40.0	36.0	41.0	32.0	41.0
17	37.47942634707545	39.0	36.0	41.0	32.0	41.0
18	37.31980228211316	39.0	36.0	41.0	32.0	41.0
19	37.202826477331335	39.0	36.0	41.0	32.0	41.0
20	37.01856409831684	39.0	35.0	41.0	32.0	41.0
21	36.9508016760092	39.0	35.0	41.0	31.0	41.0
22	36.884846271707744	38.0	35.0	41.0	31.0	41.0
23	36.80818428571966	38.0	35.0	41.0	31.0	41.0
24	36.71415572730592	38.0	35.0	40.0	31.0	41.0
25	36.61717269445208	38.0	35.0	40.0	31.0	41.0
26	36.47298093972466	38.0	35.0	40.0	31.0	41.0
27	36.37991386547504	38.0	35.0	40.0	30.0	41.0
28	36.25704755845515	38.0	35.0	40.0	30.0	41.0
29	36.247308484326	38.0	35.0	40.0	30.0	41.0
30	36.13977397216439	38.0	35.0	40.0	30.0	41.0
31	35.97197352718621	38.0	35.0	40.0	30.0	41.0
32	35.78693864797407	38.0	35.0	40.0	29.0	41.0
33	35.55438408920646	38.0	34.0	40.0	27.0	41.0
34	35.33596191709552	38.0	34.0	40.0	25.0	41.0
35	35.137398496410434	38.0	34.0	40.0	25.0	41.0
36	35.06145630592815	38.0	34.0	40.0	24.0	41.0
37	34.99704853725657	38.0	34.0	40.0	24.0	41.0
38	34.894508924033715	38.0	34.0	40.0	24.0	41.0
39	34.82203921981998	38.0	34.0	40.0	23.0	41.0
40	34.74135435999563	38.0	34.0	40.0	23.0	41.0
41	34.63040157210567	38.0	34.0	40.0	23.0	41.0
42	34.58455677236467	38.0	33.0	40.0	23.0	41.0
43	34.54189157139039	38.0	33.0	40.0	23.0	41.0
44	34.41468729176941	38.0	33.0	40.0	23.0	41.0
45	34.31065500638103	37.0	33.0	40.0	23.0	41.0
46	34.28425296746238	37.0	33.0	40.0	23.0	41.0
47	34.22857535453317	37.0	33.0	40.0	23.0	41.0
48	34.132466843101895	37.0	33.0	40.0	22.0	41.0
49	34.04863212501553	37.0	33.0	40.0	22.0	41.0
50	33.94587755194236	37.0	33.0	40.0	22.0	41.0
51	33.38228218844939	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	9.0
14	14.0
15	58.0
16	220.0
17	495.0
18	1267.0
19	2520.0
20	4397.0
21	7172.0
22	11039.0
23	16760.0
24	25111.0
25	37749.0
26	50760.0
27	57062.0
28	58691.0
29	62672.0
30	71481.0
31	83398.0
32	100486.0
33	125536.0
34	183788.0
35	245268.0
36	216030.0
37	264404.0
38	380964.0
39	648646.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.903697234133066	23.34919493583204	27.318611156077417	18.428496673957483
2	34.620921503890735	24.209900199901366	28.687577880593757	12.48160041561414
3	27.171602712032854	24.74315874276722	35.375577398722285	12.709661146477632
4	24.096434527596553	27.475595845364438	34.80711965094436	13.620849976094657
5	21.944426667068225	31.276469990324923	33.2637756888315	13.51532765377535
6	20.231674766875855	39.72917317632355	29.89831759094383	10.140834465856772
7	85.47308107863917	2.8545614028483124	10.054587002270067	1.6177705162424567
8	86.11280309903589	2.272776897274791	9.654332513900862	1.960087489788466
9	80.59925234629995	5.317903407358328	11.55625661161536	2.5265876347263685
10	35.86064879475664	37.242979923277026	16.48967929195011	10.406691990016226
11	25.853496015148835	24.656196001219737	33.12888932391174	16.361418659719686
12	25.77044847928141	22.96877999932237	32.969307046240836	18.291464475155383
13	22.101938403273714	26.41909265108365	33.79793774070045	17.681031204942194
14	17.678923017268318	30.021797154699563	33.83302400698713	18.46625582104498
15	17.306865539037236	26.703735633265698	38.583448467987544	17.40595035970952
16	21.094902326912145	25.43822068960325	36.84641476333711	16.6204622201475
17	19.927116940417346	25.447067548591846	32.984139652374914	21.6416758586159
18	20.82494136603032	25.47247873930377	34.87303816196152	18.829541732704392
19	23.102085223486714	27.807823635042595	31.114478355312446	17.975612786158244
20	24.288881945254882	27.500479989157895	30.53314560424047	17.677492461346755
21	22.31027252090306	27.203790220267965	32.89936039091823	17.586576867910747
22	21.51202231667238	24.40927451991673	32.90899782028453	21.169705343126367
23	18.990027519378387	28.30091367347938	32.5359991868419	20.17305962030034
24	19.10688135044479	26.94534899917555	35.934811825427005	18.01295782495266
25	20.209727027342442	28.156803987486402	32.31140190715692	19.32206707801424
26	18.55201388392168	30.899254981534536	30.740500920449797	19.80823021409399
27	19.129883183815142	28.74562833404234	32.56750906332469	19.556979418817832
28	18.174610644088983	28.47005808809966	35.032131038922415	18.323200228888947
29	19.330612767335136	26.148454058449506	34.47007314658304	20.050860027632318
30	20.968825174772523	26.793898302532458	33.397570313705856	18.839706208989163
31	22.852716738633667	27.022900188607508	30.77690480403266	19.347478268726164
32	23.903798878895913	27.61729617401583	30.2395804706529	18.239324476435357
33	22.425771088464824	27.29429170541089	30.849223170488383	19.4307140356359
34	21.132096780872715	28.238797429516886	31.06794764165327	19.561158147957126
35	20.42303044448878	28.55013157349858	31.36561621196321	19.66122177004943
36	24.105206094168224	28.336150524599912	28.941840372546878	18.61680300868498
37	21.003083224473045	30.75330063132692	29.793021145875294	18.450594998324743
38	21.214203161528587	30.647477139339912	29.019617439229606	19.118702259901895
39	20.562810816508616	30.297405046850706	28.72624053668435	20.41354359995633
40	22.60907047746686	28.502960874295546	29.288034905564487	19.599933742673105
41	19.557167649860144	28.522800426155083	29.70929597825555	22.210735945729226
42	21.388956861209724	29.094156931984593	28.300876027270917	21.21601017953477
43	20.994010488233677	28.427819042205165	29.76162420801789	20.81654626154327
44	20.494407655732953	30.1196396504926	29.178559731356657	20.20739296241779
45	19.7604195293471	30.264201090987118	28.64210126077152	21.333278118894256
46	21.17079708317177	29.35873448505634	28.813052693397985	20.65741573837391
47	20.5020121898423	28.69872115829854	30.027481732177343	20.771784919681817
48	20.877194303375735	28.056627426768717	30.654516980322327	20.411661289533225
49	21.04106824881132	27.652871841012534	30.072845413374193	21.233214496801956
50	20.02469591275115	29.094834563736917	30.33945586170289	20.541013661809053
51	19.527276560341225	29.682416585413602	29.443061992011476	21.3472448622337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2939.0
1	3126.0
2	3313.0
3	29713.5
4	56114.0
5	37555.0
6	18996.0
7	19221.5
8	19447.0
9	21180.0
10	22913.0
11	23751.5
12	24590.0
13	24804.0
14	25018.0
15	24482.0
16	23946.0
17	23654.0
18	23362.0
19	21750.0
20	20138.0
21	20208.5
22	20279.0
23	20637.5
24	20996.0
25	24695.0
26	32840.5
27	37287.0
28	40199.5
29	43112.0
30	49233.0
31	55354.0
32	62794.0
33	70234.0
34	79009.5
35	87785.0
36	94868.5
37	101952.0
38	111089.0
39	120226.0
40	137318.0
41	154410.0
42	164095.0
43	173780.0
44	184641.0
45	195502.0
46	206846.5
47	218191.0
48	221524.0
49	224857.0
50	212752.0
51	200647.0
52	178868.5
53	157090.0
54	143789.5
55	130489.0
56	116845.5
57	103202.0
58	92641.0
59	82080.0
60	71489.5
61	60899.0
62	52971.0
63	45043.0
64	37539.5
65	30036.0
66	24559.0
67	19082.0
68	15643.0
69	12204.0
70	10566.5
71	8929.0
72	7497.5
73	6066.0
74	4698.0
75	2724.5
76	2119.0
77	1547.5
78	976.0
79	776.0
80	576.0
81	414.0
82	252.0
83	158.5
84	65.0
85	58.5
86	52.0
87	30.5
88	9.0
89	15.0
90	21.0
91	14.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2656310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.003210808919704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33923026091541	16.797081764215843
2	7.530513862071593	3.313909680133067
3	2.9928409488618275	1.975563309461222
4	1.8134869791311619	1.596101452042157
5	1.2358013591075845	1.3595798911196835
6	0.8751920994372954	1.1554221757331895
7	0.6769732043795145	1.042690888956664
8	0.5616184581536648	0.9885927463148431
9	0.4672280537625658	0.9252465626501106
>10	5.289348493613523	27.595448424011153
>50	1.7463076553627659	27.14108296793872
>100	0.46677886187976	13.384584317398987
>500	0.00242654394541821	0.33907580006769555
>1k	0.0020798948103584653	0.8977442732643744
>5k	0.0	0.0
>10k+	1.7332456752987212E-4	1.4878757466922816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39014	1.4687291769409443	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT	4182	0.15743644378856383	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	2949	0.11101866875477637	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	2796	0.1052587988600728	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7646208462114736E-5	0.0	0.0	0.04705776057764342	0.0
2	3.7646208462114736E-5	0.0	0.0	0.12995471161122008	0.0
3	3.7646208462114736E-5	0.0	0.0	0.2718056250964684	0.0
4	3.7646208462114736E-5	0.0	0.0	0.4458440468168248	0.0
5	3.7646208462114736E-5	0.0	0.0	0.771370811388731	0.0
6	3.7646208462114736E-5	0.0	0.0	1.5382617239704703	0.0
7	3.7646208462114736E-5	0.0	0.0	1.9914467814374075	0.0
8	3.7646208462114736E-5	0.0	0.0	2.875379756127862	0.0
9	3.7646208462114736E-5	0.0	0.0	3.3748696500032	0.0
10	3.7646208462114736E-5	0.0	0.0	3.917916207069205	0.0
11	3.7646208462114736E-5	0.0	0.0	4.333643287116338	0.0
12	3.7646208462114736E-5	0.0	0.0	4.666134600253735	0.0
13	3.7646208462114736E-5	0.0	0.0	4.881772082324729	0.0
14	3.7646208462114736E-5	0.0	0.0	5.021477161927637	0.0
15	3.7646208462114736E-5	0.0	0.0	5.128618271210815	0.0
16	3.7646208462114736E-5	0.0	0.0	5.275589069046911	0.0
17	3.7646208462114736E-5	0.0	0.0	5.450154537685737	0.0
18	3.7646208462114736E-5	0.0	0.0	5.662592092037451	0.0
19	3.7646208462114736E-5	0.0	0.0	5.814494543182083	0.0
20	3.7646208462114736E-5	0.0	0.0	5.9730227270160485	0.0
21	3.7646208462114736E-5	0.0	0.0	6.144275329310209	0.0
22	3.7646208462114736E-5	0.0	0.0	6.346021360458681	0.0
23	3.7646208462114736E-5	0.0	0.0	6.561847073571985	0.0
24	3.7646208462114736E-5	0.0	0.0	6.740290101682409	0.0
25	3.7646208462114736E-5	0.0	0.0	6.889293794775459	0.0
26	3.7646208462114736E-5	0.0	0.0	7.0377327947415775	0.0
27	3.7646208462114736E-5	0.0	0.0	7.196486855826316	0.0
28	3.7646208462114736E-5	0.0	0.0	7.366195963573529	0.0
29	3.7646208462114736E-5	0.0	0.0	7.557815164645692	0.0
30	3.7646208462114736E-5	0.0	0.0	7.8144117215234665	0.0
31	3.7646208462114736E-5	0.0	0.0	8.01472719675038	0.0
32	3.7646208462114736E-5	0.0	0.0	8.219484924575822	0.0
33	3.7646208462114736E-5	0.0	0.0	8.413024082279554	0.0
34	3.7646208462114736E-5	0.0	0.0	8.613979543050322	0.0
35	3.7646208462114736E-5	0.0	0.0	8.83379575426061	0.0
36	3.7646208462114736E-5	0.0	0.0	9.041640471179946	0.0
37	3.7646208462114736E-5	0.0	0.0	9.254906242117825	0.0
38	3.7646208462114736E-5	0.0	0.0	9.504651189055494	0.0
39	3.7646208462114736E-5	0.0	0.0	9.867146530337196	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGC	25	3.8921935E-5	44.999996	34
CGTTTTT	19525	0.0	43.236874	1
TAACGCG	45	1.9299478E-8	40.0	1
TATTGCG	215	0.0	38.720932	1
CGTTAGG	355	0.0	38.661972	2
TAGGGAC	3500	0.0	38.314285	5
CGACCCG	525	0.0	37.714283	32
TATGGGA	3620	0.0	37.665745	4
CGAGGGA	1915	0.0	37.59791	4
TAGGGAT	5285	0.0	37.251656	5
TAGGGCG	980	0.0	37.19388	5
ATAGGGA	4700	0.0	37.101063	4
CACGACC	655	0.0	37.099236	27
AGGGATT	6120	0.0	36.985294	6
GTTTTTT	23160	0.0	36.85881	2
ACGGGAT	1635	0.0	36.74312	5
TAAGGGA	3895	0.0	36.73941	4
GGGCGAT	4615	0.0	36.663055	7
GGCGATT	1235	0.0	36.072876	8
TACGGGA	1285	0.0	36.070038	4
>>END_MODULE